FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4288439568> ?p ?o ?g. }

• W4288439568 endingPage "11046" @default.
• W4288439568 startingPage "11038" @default.
• W4288439568 abstract "Protein networks can be assembled in vitro for basic biochemistry research, drug screening, and the creation of artificial cells. Two standard methodologies are used: manual pipetting and pipetting robots. Manual pipetting has limited throughput in the number of input reagents and the combination of reagents in a single sample. While pipetting robots are evident in improving pipetting efficiency and saving hands-on time, their liquid handling volume usually ranges from a few to hundreds of microliters. Microfluidic methods have been developed to minimize the reagent consumption and speed up screening but are challenging in multifactorial protein studies due to their reliance on complex structures and labeling dyes. Here, we engineered a new impact-printing-based methodology to generate printed microdroplet arrays containing water-in-oil droplets. The printed droplet volume was linearly proportional (R2 = 0.9999) to the single droplet number, and each single droplet volume was around 59.2 nL (coefficient of variation = 93.8%). Our new methodology enables the study of protein networks in both membrane-unbound and -bound states, without and with anchor lipids DGS-NTA(Ni), respectively. The methodology is demonstrated using a subnetwork of mitogen-activated protein kinase (MAPK). It takes less than 10 min to prepare 100 different droplet-based reactions, using <1 μL reaction volume at each reaction site. We validate the kinase (ATPase) activity of MEK1 (R4F)* and ERK2 WT individually and together under different concentrations, without and with the selective membrane attachment. Our new methodology provides a reagent-saving, efficient, and flexible way for protein network research and related applications." @default.
• W4288439568 created "2022-07-29" @default.
• W4288439568 creator A5011963844 @default.
• W4288439568 creator A5012995172 @default.
• W4288439568 creator A5013292013 @default.
• W4288439568 creator A5027200477 @default.
• W4288439568 creator A5030089851 @default.
• W4288439568 creator A5075585957 @default.
• W4288439568 creator A5084360323 @default.
• W4288439568 creator A5091497474 @default.
• W4288439568 date "2022-07-28" @default.
• W4288439568 modified "2023-10-15" @default.
• W4288439568 title "Microfluidic Printing-Based Method for the Multifactorial Study of Cell-Free Protein Networks" @default.
• W4288439568 cites W1532900970 @default.
• W4288439568 cites W1963506890 @default.
• W4288439568 cites W1967179899 @default.
• W4288439568 cites W1973707938 @default.
• W4288439568 cites W2007783125 @default.
• W4288439568 cites W2025915013 @default.
• W4288439568 cites W2030427930 @default.
• W4288439568 cites W2031732057 @default.
• W4288439568 cites W2032977054 @default.
• W4288439568 cites W2040331257 @default.
• W4288439568 cites W2059307930 @default.
• W4288439568 cites W2070287747 @default.
• W4288439568 cites W2071222048 @default.
• W4288439568 cites W2090799728 @default.
• W4288439568 cites W2093621953 @default.
• W4288439568 cites W2118131251 @default.
• W4288439568 cites W2144523043 @default.
• W4288439568 cites W2261521410 @default.
• W4288439568 cites W2291475213 @default.
• W4288439568 cites W2321432786 @default.
• W4288439568 cites W2329014569 @default.
• W4288439568 cites W2335345046 @default.
• W4288439568 cites W2561554545 @default.
• W4288439568 cites W2595573636 @default.
• W4288439568 cites W2766036492 @default.
• W4288439568 cites W2804925552 @default.
• W4288439568 cites W2888328180 @default.
• W4288439568 cites W2897838791 @default.
• W4288439568 cites W2922564053 @default.
• W4288439568 cites W2952159850 @default.
• W4288439568 cites W2973189200 @default.
• W4288439568 cites W3020961372 @default.
• W4288439568 cites W3022153092 @default.
• W4288439568 cites W3094501548 @default.
• W4288439568 cites W3155035950 @default.
• W4288439568 doi "https://doi.org/10.1021/acs.analchem.2c01851" @default.
• W4288439568 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35901235" @default.
• W4288439568 hasPublicationYear "2022" @default.
• W4288439568 type Work @default.
• W4288439568 citedByCount "0" @default.
• W4288439568 crossrefType "journal-article" @default.
• W4288439568 hasAuthorship W4288439568A5011963844 @default.
• W4288439568 hasAuthorship W4288439568A5012995172 @default.
• W4288439568 hasAuthorship W4288439568A5013292013 @default.
• W4288439568 hasAuthorship W4288439568A5027200477 @default.
• W4288439568 hasAuthorship W4288439568A5030089851 @default.
• W4288439568 hasAuthorship W4288439568A5075585957 @default.
• W4288439568 hasAuthorship W4288439568A5084360323 @default.
• W4288439568 hasAuthorship W4288439568A5091497474 @default.
• W4288439568 hasConcept C121332964 @default.
• W4288439568 hasConcept C12554922 @default.
• W4288439568 hasConcept C147789679 @default.
• W4288439568 hasConcept C151552171 @default.
• W4288439568 hasConcept C157764524 @default.
• W4288439568 hasConcept C171250308 @default.
• W4288439568 hasConcept C178790620 @default.
• W4288439568 hasConcept C184235292 @default.
• W4288439568 hasConcept C185592680 @default.
• W4288439568 hasConcept C192562407 @default.
• W4288439568 hasConcept C20556612 @default.
• W4288439568 hasConcept C40875361 @default.
• W4288439568 hasConcept C41008148 @default.
• W4288439568 hasConcept C41625074 @default.
• W4288439568 hasConcept C43617362 @default.
• W4288439568 hasConcept C55493867 @default.
• W4288439568 hasConcept C555944384 @default.
• W4288439568 hasConcept C62520636 @default.
• W4288439568 hasConcept C76155785 @default.
• W4288439568 hasConcept C8673954 @default.
• W4288439568 hasConcept C86803240 @default.
• W4288439568 hasConcept C97029542 @default.
• W4288439568 hasConceptScore W4288439568C121332964 @default.
• W4288439568 hasConceptScore W4288439568C12554922 @default.
• W4288439568 hasConceptScore W4288439568C147789679 @default.
• W4288439568 hasConceptScore W4288439568C151552171 @default.
• W4288439568 hasConceptScore W4288439568C157764524 @default.
• W4288439568 hasConceptScore W4288439568C171250308 @default.
• W4288439568 hasConceptScore W4288439568C178790620 @default.
• W4288439568 hasConceptScore W4288439568C184235292 @default.
• W4288439568 hasConceptScore W4288439568C185592680 @default.
• W4288439568 hasConceptScore W4288439568C192562407 @default.
• W4288439568 hasConceptScore W4288439568C20556612 @default.
• W4288439568 hasConceptScore W4288439568C40875361 @default.
• W4288439568 hasConceptScore W4288439568C41008148 @default.
• W4288439568 hasConceptScore W4288439568C41625074 @default.

• next