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- W4288601919 abstract "In-cell distance determination by EPR reveals essential structural information about biomacromolecules under native conditions. We demonstrate that the pulsed EPR technique RIDME (relaxation induced dipolar modulation enhancement) can be utilized for such distance determination. The performance of in-cell RIDME has been assessed at Q band using stiff molecular rulers labelled with Gd(III)-PyMTA tags and microinjected into X. laevis oocytes. The overtone coefficients are determined to be the same for protonated aqueous solutions and inside cells. As compared to in-cell DEER (double electron-electron resonance, also abbreviated as PELDOR), in-cell RIDME features approximately 5 times larger modulation depth and does not show artificial broadening in the distance distributions due to the effect of pseudo-secular terms." @default.
- W4288601919 created "2022-07-29" @default.
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- W4288601919 date "2019-02-04" @default.
- W4288601919 modified "2023-09-26" @default.
- W4288601919 title "Gd(III)-Gd(III) RIDME for In-Cell EPR Distance Determination" @default.
- W4288601919 doi "https://doi.org/10.48550/arxiv.1902.01254" @default.
- W4288601919 hasPublicationYear "2019" @default.
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