FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4288695778> ?p ?o ?g. }

• W4288695778 endingPage "4254" @default.
• W4288695778 startingPage "4241" @default.
• W4288695778 abstract "Abstract The Fanconi anemia (FA) and homologous recombination (HR) pathways, which partially overlap and include RAD51 and its paralogs, are key for the repair of different types of DNA damage, such as DNA interstrand crosslinks. First, to broadly assess the impact of microRNA-mediated regulation, we examined microRNA expression profiles in five isogenic fibroblast cell pairs, either deficient in DNA repair due to germline mutations in FANCA, FANCB, FANCC, FANCI or BRIP1/FANCJ or proficient due to correction with retroviral vectors. In each pair, we observed lower abundance of specific microRNAs in the FA-deficient cells. From the list of microRNAs, we experimentally confirmed the effects of miR-141-3p and miR-369-3p targeting RAD51B and miR-15a-5p, miR-494-3p as well as miR-544a targeting RAD51D. However, by western blotting, only RAD51D protein was reduced by a mixture of its regulating microRNAs. Gene ontology analyses and identification of additional FA/HR factors as targets of miR-15a-5p, miR-494-3p and miR-544a strongly suggested the widespread influence of these microRNAs on HR. Interestingly, only miR-494-3p directly reduced RAD51 foci formation, while a mixture of miR-15a-5p, miR-494-3p and miR-544a strongly reduced HR activity in green fluorescent protein (GFP) repair assays. In summary, by successfully employing this novel loss- and gain-of-function strategy, we have identified new microRNAs strongly inhibiting HR in mammalian cells. Understanding and modulating such miRNA regulation of DNA repair genes/pathways might help to overcome the reduced repair capacity of FA patients with biallelic hypomorphic mutations or help to engineer synthetic lethality strategies for patients with mutations in cancer-associated FA/HR genes." @default.
• W4288695778 created "2022-07-30" @default.
• W4288695778 creator A5010286459 @default.
• W4288695778 creator A5012966524 @default.
• W4288695778 creator A5017322730 @default.
• W4288695778 creator A5020192294 @default.
• W4288695778 creator A5041930158 @default.
• W4288695778 creator A5042253832 @default.
• W4288695778 creator A5042874282 @default.
• W4288695778 creator A5053144484 @default.
• W4288695778 creator A5053447705 @default.
• W4288695778 creator A5075811425 @default.
• W4288695778 creator A5080667810 @default.
• W4288695778 date "2022-07-29" @default.
• W4288695778 modified "2023-09-26" @default.
• W4288695778 title "Identification of new RAD51D-regulating microRNAs that also emerge as potent inhibitors of the Fanconi anemia/homologous recombination pathways" @default.
• W4288695778 cites W1261503999 @default.
• W4288695778 cites W1632221008 @default.
• W4288695778 cites W1924945038 @default.
• W4288695778 cites W1965306643 @default.
• W4288695778 cites W1978337436 @default.
• W4288695778 cites W1978631361 @default.
• W4288695778 cites W1985097719 @default.
• W4288695778 cites W1985886710 @default.
• W4288695778 cites W1985915478 @default.
• W4288695778 cites W2000067413 @default.
• W4288695778 cites W2002495174 @default.
• W4288695778 cites W2003058744 @default.
• W4288695778 cites W2004125597 @default.
• W4288695778 cites W2007138966 @default.
• W4288695778 cites W2011798292 @default.
• W4288695778 cites W2016294195 @default.
• W4288695778 cites W2040881136 @default.
• W4288695778 cites W2041205258 @default.
• W4288695778 cites W2044832480 @default.
• W4288695778 cites W2049689768 @default.
• W4288695778 cites W2050869737 @default.
• W4288695778 cites W2052215590 @default.
• W4288695778 cites W2055532978 @default.
• W4288695778 cites W2068655274 @default.
• W4288695778 cites W2078210863 @default.
• W4288695778 cites W2083381199 @default.
• W4288695778 cites W2103770333 @default.
• W4288695778 cites W2106671241 @default.
• W4288695778 cites W2106929712 @default.
• W4288695778 cites W2107374121 @default.
• W4288695778 cites W2120074470 @default.
• W4288695778 cites W2127686290 @default.
• W4288695778 cites W2128700722 @default.
• W4288695778 cites W2131978108 @default.
• W4288695778 cites W2133465414 @default.
• W4288695778 cites W2134629862 @default.
• W4288695778 cites W2135195813 @default.
• W4288695778 cites W2135785842 @default.
• W4288695778 cites W2137734450 @default.
• W4288695778 cites W2138255429 @default.
• W4288695778 cites W2138954682 @default.
• W4288695778 cites W2146642335 @default.
• W4288695778 cites W2151206132 @default.
• W4288695778 cites W2156010403 @default.
• W4288695778 cites W2158217645 @default.
• W4288695778 cites W2159793493 @default.
• W4288695778 cites W2161222316 @default.
• W4288695778 cites W2162634903 @default.
• W4288695778 cites W2171039798 @default.
• W4288695778 cites W2218955305 @default.
• W4288695778 cites W2226761276 @default.
• W4288695778 cites W2234731040 @default.
• W4288695778 cites W2266334531 @default.
• W4288695778 cites W2378444228 @default.
• W4288695778 cites W2401802453 @default.
• W4288695778 cites W2413931725 @default.
• W4288695778 cites W2482436688 @default.
• W4288695778 cites W2560940732 @default.
• W4288695778 cites W2617770423 @default.
• W4288695778 cites W2767959173 @default.
• W4288695778 cites W2770093340 @default.
• W4288695778 cites W2790652014 @default.
• W4288695778 cites W2800468880 @default.
• W4288695778 cites W2803643617 @default.
• W4288695778 cites W2897409189 @default.
• W4288695778 cites W2900464141 @default.
• W4288695778 cites W2900810167 @default.
• W4288695778 cites W2903449267 @default.
• W4288695778 cites W2915433652 @default.
• W4288695778 cites W2920406367 @default.
• W4288695778 cites W2928369260 @default.
• W4288695778 cites W2950595506 @default.
• W4288695778 cites W2971467935 @default.
• W4288695778 cites W2981672224 @default.
• W4288695778 cites W2989883225 @default.
• W4288695778 cites W3000696048 @default.
• W4288695778 cites W3003690518 @default.
• W4288695778 cites W3007516256 @default.
• W4288695778 cites W3007550231 @default.
• W4288695778 cites W3042430778 @default.
• W4288695778 cites W3131539356 @default.
• W4288695778 cites W3154159901 @default.

• next