Matches in SemOpenAlex for { <https://semopenalex.org/work/W4288698518> ?p ?o ?g. }
- W4288698518 abstract "This study aimed to investigate the impact of CC chemokine receptor 7 (CCR7) on the recruitment and polarization of tumor-associated macrophages (TAMs) in oral squamous cell carcinoma (OSCC).We analyzed CCR7 expression pattern, clinicopathological significance, and its association with M2 macrophage infiltration in OSCC by bioinformatic methods. Small interfering RNA (siRNA) was utilized to silence CCR7 in OSCC cells. Conditioned media (CM) was harvested from transfected OSCC cells to establish a co-culture model of THP-1 derived macrophages and OSCC cells. Transwell assay and cell adhesion assay were performed to examine the effect of CCR7 on macrophages recruitment and adhesion. Cytoskeleton was labelled by phalloidin to observe macrophage morphological changes. Moreover, phenotypic alteration of macrophages was measured using quantitative real-time PCR (qRT-PCR), flow cytometry, and immunofluorescence (IF) staining. Ultimately, recombinant human CCL19 and CCL21 were added into the medium of THP-1 derived macrophages to explore their effects on polarization in vitro.In OSCC patients, the overexpression of CCR7 positively correlated with lymph node metastasis and M2 macrophage infiltration. Macrophage not only exhibited enhanced migration, invasion and adhesion abilities, but also appeared more spindle and branched in vitro when treated with CM from OSCC cells. However, these phenomena were abrogated with knockdown of CCR7. We also discovered that inhibition of CCR7 in OSCC cells suppressed TAMs polarization to an M2 phenotype. In addition, recombinant human CCL19 and CCL21 promoted macrophage M2-polarization in vitro.CCR7 in OSCC cells promoted recruitment and M2-polarization of THP-1 derived macrophages in vitro by regulating production of CCL19 and CCL21." @default.
- W4288698518 created "2022-07-30" @default.
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- W4288698518 date "2022-07-29" @default.
- W4288698518 modified "2023-10-04" @default.
- W4288698518 title "CC chemokine receptor 7 promotes macrophage recruitment and induces M2-polarization through CC chemokine ligand 19&21 in oral squamous cell carcinoma" @default.
- W4288698518 cites W1558612181 @default.
- W4288698518 cites W1639480315 @default.
- W4288698518 cites W1768839940 @default.
- W4288698518 cites W1963904011 @default.
- W4288698518 cites W1971620413 @default.
- W4288698518 cites W1983220630 @default.
- W4288698518 cites W1997397497 @default.
- W4288698518 cites W2002621414 @default.
- W4288698518 cites W2003566453 @default.
- W4288698518 cites W2007985202 @default.
- W4288698518 cites W2020541351 @default.
- W4288698518 cites W2022131506 @default.
- W4288698518 cites W2031405609 @default.
- W4288698518 cites W2064949716 @default.
- W4288698518 cites W2076462567 @default.
- W4288698518 cites W2103307831 @default.
- W4288698518 cites W2105207470 @default.
- W4288698518 cites W2107263674 @default.
- W4288698518 cites W2107551122 @default.
- W4288698518 cites W2121391430 @default.
- W4288698518 cites W2128490578 @default.
- W4288698518 cites W2130430382 @default.
- W4288698518 cites W2137132605 @default.
- W4288698518 cites W2139695709 @default.
- W4288698518 cites W2141278603 @default.
- W4288698518 cites W2147425799 @default.
- W4288698518 cites W2153960708 @default.
- W4288698518 cites W2161903417 @default.
- W4288698518 cites W2166642248 @default.
- W4288698518 cites W2236553376 @default.
- W4288698518 cites W2345827279 @default.
- W4288698518 cites W2406015015 @default.
- W4288698518 cites W2413017918 @default.
- W4288698518 cites W2525253403 @default.
- W4288698518 cites W2535284666 @default.
- W4288698518 cites W2598936163 @default.
- W4288698518 cites W2600317738 @default.
- W4288698518 cites W2604261328 @default.
- W4288698518 cites W2612051769 @default.
- W4288698518 cites W2617691667 @default.
- W4288698518 cites W2749613778 @default.
- W4288698518 cites W2757832734 @default.
- W4288698518 cites W2765224588 @default.
- W4288698518 cites W2778071905 @default.
- W4288698518 cites W2792583114 @default.
- W4288698518 cites W2802519389 @default.
- W4288698518 cites W2886498561 @default.
- W4288698518 cites W2895742304 @default.
- W4288698518 cites W2896837916 @default.
- W4288698518 cites W2915413496 @default.
- W4288698518 cites W2918952436 @default.
- W4288698518 cites W2946155588 @default.
- W4288698518 cites W2947975256 @default.
- W4288698518 cites W2960078024 @default.
- W4288698518 cites W2963317183 @default.
- W4288698518 cites W2974291499 @default.
- W4288698518 cites W3002834433 @default.
- W4288698518 cites W3003918022 @default.
- W4288698518 cites W3004509808 @default.
- W4288698518 cites W3005023764 @default.
- W4288698518 cites W3016720267 @default.
- W4288698518 cites W3020088622 @default.
- W4288698518 cites W3027286012 @default.
- W4288698518 cites W3035865799 @default.
- W4288698518 cites W3039994783 @default.
- W4288698518 cites W3042975207 @default.
- W4288698518 cites W3100029288 @default.
- W4288698518 cites W3117666406 @default.
- W4288698518 cites W3119005666 @default.
- W4288698518 cites W3126764173 @default.
- W4288698518 cites W3128646645 @default.
- W4288698518 cites W3171155436 @default.
- W4288698518 cites W3173665490 @default.
- W4288698518 cites W3179498515 @default.
- W4288698518 cites W3190675264 @default.
- W4288698518 cites W4200231135 @default.
- W4288698518 cites W4200354421 @default.
- W4288698518 cites W4206978722 @default.
- W4288698518 cites W4210694027 @default.
- W4288698518 cites W4213102595 @default.
- W4288698518 cites W4313335032 @default.
- W4288698518 doi "https://doi.org/10.1007/s12672-022-00533-x" @default.
- W4288698518 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35904690" @default.