FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4288734439> ?p ?o ?g. }

• W4288734439 endingPage "102753" @default.
• W4288734439 startingPage "102753" @default.
• W4288734439 abstract "The Ion Torrent ™ Genexus ™ Sequencer (Genexus) is a highly integrated instrument that can automate library construction, templating, and sequencing in a single-instrument run. By programing the ForeNGS Analysis Software (FNAS), we bridged the gap between sequencing and genotyping without manual intervention. FNAS can automatically transfer sequencing output files from Genexus, analyze the repeat and flanking regions aligned to the GRCh38 assembly, name the alleles according to the ISFG guidelines, and generate user-friendly interactive profiles. Genexus and FNAS can accomplish the fully automatic DNA-to-Profile workflow in forensics. Based on our experiences, the optimal assay parameters on Genexus were validated as follows: 24 cycles of target amplification for library construction; 40 μL of library and 400 bp of template size for templating; 852 flows of dNTPs by order of Ion samba HID2 for sequencing; and 750,000 reads per sample at minimum for 16 samples multiplexed on a lane. By developmental validations of the Precision ID Globalfiler ™ NGS STR Panel v2, Genexus presented competitive performance at the optimal assay parameters qualified to detect commonly used forensic STR markers. It could produce repeatable and reproducible results, and human profiles could be easily separated from nonhuman profiles. Additionally, Genexus was sensitive enough to detect samples with 100 pg of input DNA, and it was suitable for various types of case samples, especially for low copy number samples and degraded samples. Moreover, minor contributors could be detected between the 4:1 and 1:4 mixtures with an analysis threshold of 50 × . The Genexus workflow is a robust and labor-effective solution enabling forensic scientists to obtain NGS-STR profiles within a single day and with only the need to prepare DNA extracts, then set up Genexus, and finally interpret profiles on FNAS." @default.
• W4288734439 created "2022-07-30" @default.
• W4288734439 creator A5010077863 @default.
• W4288734439 creator A5020141096 @default.
• W4288734439 creator A5021443021 @default.
• W4288734439 creator A5029030051 @default.
• W4288734439 creator A5037277189 @default.
• W4288734439 creator A5042955002 @default.
• W4288734439 creator A5064890470 @default.
• W4288734439 creator A5079646066 @default.
• W4288734439 date "2022-11-01" @default.
• W4288734439 modified "2023-09-24" @default.
• W4288734439 title "Ion Torrent ™ Genexus ™ Integrated Sequencer and ForeNGS Analysis Software—An automatic NGS-STR workflow from DNA to profile for forensic science" @default.
• W4288734439 cites W1977804928 @default.
• W4288734439 cites W2030702501 @default.
• W4288734439 cites W2037315145 @default.
• W4288734439 cites W2068875547 @default.
• W4288734439 cites W2072538373 @default.
• W4288734439 cites W2105816151 @default.
• W4288734439 cites W2169853020 @default.
• W4288734439 cites W2229178177 @default.
• W4288734439 cites W2278859413 @default.
• W4288734439 cites W2285073927 @default.
• W4288734439 cites W2324280679 @default.
• W4288734439 cites W2342147735 @default.
• W4288734439 cites W2481662208 @default.
• W4288734439 cites W2532049456 @default.
• W4288734439 cites W2613050520 @default.
• W4288734439 cites W2752508545 @default.
• W4288734439 cites W2756319513 @default.
• W4288734439 cites W2791890676 @default.
• W4288734439 cites W2793968990 @default.
• W4288734439 cites W2797169829 @default.
• W4288734439 cites W2848145301 @default.
• W4288734439 cites W2913434445 @default.
• W4288734439 cites W2953284690 @default.
• W4288734439 cites W2967307997 @default.
• W4288734439 cites W3036874752 @default.
• W4288734439 cites W3081290172 @default.
• W4288734439 cites W3099155666 @default.
• W4288734439 cites W3120574451 @default.
• W4288734439 cites W3152424289 @default.
• W4288734439 cites W3166426295 @default.
• W4288734439 cites W3197206627 @default.
• W4288734439 cites W3210087778 @default.
• W4288734439 cites W4200607789 @default.
• W4288734439 cites W4206398220 @default.
• W4288734439 doi "https://doi.org/10.1016/j.fsigen.2022.102753" @default.
• W4288734439 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35931007" @default.
• W4288734439 hasPublicationYear "2022" @default.
• W4288734439 type Work @default.
• W4288734439 citedByCount "0" @default.
• W4288734439 crossrefType "journal-article" @default.
• W4288734439 hasAuthorship W4288734439A5010077863 @default.
• W4288734439 hasAuthorship W4288734439A5020141096 @default.
• W4288734439 hasAuthorship W4288734439A5021443021 @default.
• W4288734439 hasAuthorship W4288734439A5029030051 @default.
• W4288734439 hasAuthorship W4288734439A5037277189 @default.
• W4288734439 hasAuthorship W4288734439A5042955002 @default.
• W4288734439 hasAuthorship W4288734439A5064890470 @default.
• W4288734439 hasAuthorship W4288734439A5079646066 @default.
• W4288734439 hasConcept C104317684 @default.
• W4288734439 hasConcept C111919701 @default.
• W4288734439 hasConcept C124101348 @default.
• W4288734439 hasConcept C135763542 @default.
• W4288734439 hasConcept C164928082 @default.
• W4288734439 hasConcept C177212765 @default.
• W4288734439 hasConcept C185592680 @default.
• W4288734439 hasConcept C192800701 @default.
• W4288734439 hasConcept C198531522 @default.
• W4288734439 hasConcept C2777904410 @default.
• W4288734439 hasConcept C31467283 @default.
• W4288734439 hasConcept C41008148 @default.
• W4288734439 hasConcept C43617362 @default.
• W4288734439 hasConcept C51679486 @default.
• W4288734439 hasConcept C54355233 @default.
• W4288734439 hasConcept C552990157 @default.
• W4288734439 hasConcept C70712619 @default.
• W4288734439 hasConcept C70721500 @default.
• W4288734439 hasConcept C77088390 @default.
• W4288734439 hasConcept C86803240 @default.
• W4288734439 hasConceptScore W4288734439C104317684 @default.
• W4288734439 hasConceptScore W4288734439C111919701 @default.
• W4288734439 hasConceptScore W4288734439C124101348 @default.
• W4288734439 hasConceptScore W4288734439C135763542 @default.
• W4288734439 hasConceptScore W4288734439C164928082 @default.
• W4288734439 hasConceptScore W4288734439C177212765 @default.
• W4288734439 hasConceptScore W4288734439C185592680 @default.
• W4288734439 hasConceptScore W4288734439C192800701 @default.
• W4288734439 hasConceptScore W4288734439C198531522 @default.
• W4288734439 hasConceptScore W4288734439C2777904410 @default.
• W4288734439 hasConceptScore W4288734439C31467283 @default.
• W4288734439 hasConceptScore W4288734439C41008148 @default.
• W4288734439 hasConceptScore W4288734439C43617362 @default.
• W4288734439 hasConceptScore W4288734439C51679486 @default.
• W4288734439 hasConceptScore W4288734439C54355233 @default.
• W4288734439 hasConceptScore W4288734439C552990157 @default.
• W4288734439 hasConceptScore W4288734439C70712619 @default.

• next