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- W4288889780 abstract "The dynamic architecture of the microtubule cytoskeleton is crucial for cell division, motility and morphogenesis. The dynamic properties of microtubules - growth, shrinkage, nucleation and severing - are regulated by an arsenal of microtubule-associated proteins (MAPs). The activities of many of these MAPs have been reconstituted in vitro using microscope assays. As an alternative to fluorescence microscopy, interference-reflection microscopy (IRM) has been introduced as an easy-to-use, wide-field imaging technique that allows label-free visualization of microtubules with high contrast and speed. IRM circumvents several problems associated with fluorescence microscopy including the high concentrations of tubulin required for fluorescent labeling, the potential perturbation of function caused by the fluorophores, and the risks of photodamage. IRM can be implemented on a standard epifluorescence microscope at low cost and can be combined with fluorescence techniques like total-internal-reflection-fluorescence (TIRF) microscopy. Here we describe the experimental procedure to image microtubule dynamics and severing using IRM, providing practical tips and guidelines to resolve possible experimental hurdles." @default.
- W4288889780 created "2022-07-31" @default.
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- W4288889780 date "2021-07-03" @default.
- W4288889780 modified "2023-10-18" @default.
- W4288889780 title "In vitro reconstitution of microtubule dynamics and severing imaged by label-free interference reflection microscopy" @default.
- W4288889780 doi "https://doi.org/10.48550/arxiv.2107.01501" @default.
- W4288889780 hasPublicationYear "2021" @default.
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