Matches in SemOpenAlex for { <https://semopenalex.org/work/W4289335941> ?p ?o ?g. }
- W4289335941 endingPage "106070" @default.
- W4289335941 startingPage "106070" @default.
- W4289335941 abstract "Sulfation is a common modification of glycans and glycoproteins. Sulfated N-glycans have been identified in various glycoproteins and implicated for biological functions, but in vitro synthesis of structurally well-defined full length sulfated N-glycans remains to be described. We report here the first in vitro enzymatic sulfation of biantennary complex type N-glycans by recombinant human CHST2 (GlcNAc-6-O-sulfotransferase 1, GlcNAc6ST-1). We found that the sulfotransferase showed high antennary preference and could selectively sulfate the GlcNAc moiety located on the Manα1,3Man arm of the biantennary N-glycan. The glycan chain was further elongated by bacterial β1,4 galactosyltransferase from Neiserria meningitidis and human β1,4 galactosyltransferase IV（B4GALT4）, which led to the formation of different sulfated N-glycans. Using rituximab as a model IgG antibody, we further demonstrated that the sulfated N-glycans could be efficiently transferred to an intact antibody by using a chemoenzymatic Fc glycan remodeling method, providing homogeneous sulfated glycoforms of antibodies. Preliminary binding analysis indicated that sulfation did not affect the apparent affinity of the antibody for FcγIIIa receptor." @default.
- W4289335941 created "2022-08-02" @default.
- W4289335941 creator A5003753200 @default.
- W4289335941 creator A5024936464 @default.
- W4289335941 creator A5036627086 @default.
- W4289335941 creator A5044389560 @default.
- W4289335941 creator A5048164376 @default.
- W4289335941 creator A5071825149 @default.
- W4289335941 creator A5088464756 @default.
- W4289335941 date "2022-11-01" @default.
- W4289335941 modified "2023-10-09" @default.
- W4289335941 title "Site-selective sulfation of N-glycans by human GlcNAc-6-O-sulfotransferase 1 (CHST2) and chemoenzymatic synthesis of sulfated antibody glycoforms" @default.
- W4289335941 cites W1545659699 @default.
- W4289335941 cites W1594583996 @default.
- W4289335941 cites W1870905795 @default.
- W4289335941 cites W1988819270 @default.
- W4289335941 cites W1990194904 @default.
- W4289335941 cites W1991769156 @default.
- W4289335941 cites W1992778614 @default.
- W4289335941 cites W1998487735 @default.
- W4289335941 cites W2003650793 @default.
- W4289335941 cites W2028767015 @default.
- W4289335941 cites W2039359749 @default.
- W4289335941 cites W2039360539 @default.
- W4289335941 cites W2052373317 @default.
- W4289335941 cites W2058090031 @default.
- W4289335941 cites W2060270009 @default.
- W4289335941 cites W2068608069 @default.
- W4289335941 cites W2069001662 @default.
- W4289335941 cites W2069193445 @default.
- W4289335941 cites W2072372786 @default.
- W4289335941 cites W2077411411 @default.
- W4289335941 cites W2083548734 @default.
- W4289335941 cites W2094123105 @default.
- W4289335941 cites W2103022517 @default.
- W4289335941 cites W2105547772 @default.
- W4289335941 cites W2106984890 @default.
- W4289335941 cites W2107155846 @default.
- W4289335941 cites W2113242069 @default.
- W4289335941 cites W2119465926 @default.
- W4289335941 cites W2132632000 @default.
- W4289335941 cites W2140678357 @default.
- W4289335941 cites W2141000901 @default.
- W4289335941 cites W2157945853 @default.
- W4289335941 cites W2159159918 @default.
- W4289335941 cites W2217447765 @default.
- W4289335941 cites W2409183178 @default.
- W4289335941 cites W2587220348 @default.
- W4289335941 cites W2596270747 @default.
- W4289335941 cites W2755660236 @default.
- W4289335941 cites W2764096702 @default.
- W4289335941 cites W2777399253 @default.
- W4289335941 cites W2923750883 @default.
- W4289335941 cites W2931036886 @default.
- W4289335941 cites W2988484977 @default.
- W4289335941 cites W3167346688 @default.
- W4289335941 cites W3178619793 @default.
- W4289335941 cites W3188732328 @default.
- W4289335941 cites W4220734429 @default.
- W4289335941 doi "https://doi.org/10.1016/j.bioorg.2022.106070" @default.
- W4289335941 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35939855" @default.
- W4289335941 hasPublicationYear "2022" @default.
- W4289335941 type Work @default.
- W4289335941 citedByCount "3" @default.
- W4289335941 countsByYear W42893359412023 @default.
- W4289335941 crossrefType "journal-article" @default.
- W4289335941 hasAuthorship W4289335941A5003753200 @default.
- W4289335941 hasAuthorship W4289335941A5024936464 @default.
- W4289335941 hasAuthorship W4289335941A5036627086 @default.
- W4289335941 hasAuthorship W4289335941A5044389560 @default.
- W4289335941 hasAuthorship W4289335941A5048164376 @default.
- W4289335941 hasAuthorship W4289335941A5071825149 @default.
- W4289335941 hasAuthorship W4289335941A5088464756 @default.
- W4289335941 hasBestOaLocation W42893359411 @default.
- W4289335941 hasConcept C108625454 @default.
- W4289335941 hasConcept C155138218 @default.
- W4289335941 hasConcept C159654299 @default.
- W4289335941 hasConcept C181199279 @default.
- W4289335941 hasConcept C185592680 @default.
- W4289335941 hasConcept C203014093 @default.
- W4289335941 hasConcept C206212055 @default.
- W4289335941 hasConcept C2775904581 @default.
- W4289335941 hasConcept C2777313579 @default.
- W4289335941 hasConcept C2780895608 @default.
- W4289335941 hasConcept C55493867 @default.
- W4289335941 hasConcept C86803240 @default.
- W4289335941 hasConceptScore W4289335941C108625454 @default.
- W4289335941 hasConceptScore W4289335941C155138218 @default.
- W4289335941 hasConceptScore W4289335941C159654299 @default.
- W4289335941 hasConceptScore W4289335941C181199279 @default.
- W4289335941 hasConceptScore W4289335941C185592680 @default.
- W4289335941 hasConceptScore W4289335941C203014093 @default.
- W4289335941 hasConceptScore W4289335941C206212055 @default.
- W4289335941 hasConceptScore W4289335941C2775904581 @default.
- W4289335941 hasConceptScore W4289335941C2777313579 @default.
- W4289335941 hasConceptScore W4289335941C2780895608 @default.
- W4289335941 hasConceptScore W4289335941C55493867 @default.
- W4289335941 hasConceptScore W4289335941C86803240 @default.