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- W4289356783 endingPage "302" @default.
- W4289356783 startingPage "277" @default.
- W4289356783 abstract "Electron cryomicroscopy (cryoEM) has made great strides in the last decade, such that the atomic structure of most biological macromolecules can, at least in principle, be determined. Major technological advances - in electron imaging hardware, data analysis software, and cryogenic specimen preparation technology - continue at pace and contribute to the exponential growth in the number of atomic structures determined by cryoEM. It is now conceivable that within the next decade we will have structures for hundreds of thousands of unique protein and nucleic acid molecular complexes. But the answers to many important questions in biology would become obvious if we could identify these structures precisely inside cells with quantifiable error. In the context of an abundance of known structures, it is appropriate to consider the current state of electron cryomicroscopy for frozen specimens prepared directly from cells, and try to answer to the question of the title, both now and in the foreseeable future." @default.
- W4289356783 created "2022-08-02" @default.
- W4289356783 creator A5031770231 @default.
- W4289356783 creator A5056156146 @default.
- W4289356783 creator A5079969187 @default.
- W4289356783 date "2022-01-01" @default.
- W4289356783 modified "2023-09-27" @default.
- W4289356783 title "Cryomicroscopy <i>in situ</i>: what is the smallest molecule that can be directly identified without labels in a cell?" @default.
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