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• W4289550045 abstract "Lipase found in the gut microbiota participates in the digestion and absorption of dietary fats. As such, the gut microbiota is involved in the regulation of the host metabolism, affecting the levels of lipids and free fatty acids, ultimately resulting in obesity. In this study, an enzymatic activatable near-infrared fluorescent probe, DDAO-C6, was developed for visually sensing endogenous lipase from gut microbes. Using DDAO-C6, a cultivated intestinal yeast strain was rapidly identified from human feces that exhibited high lipase expression and was identified as Trichosporon asahii Y2. We then determined that the colonization of the gut of mice with T. asahii Y2 increased lipase activity in the digestive tract and promoted obesity and hyperlipidemia when the mice were fed high fat diets. Above all, the present research resulted in a fluorescence visualization tool for the functional investigation of gut microbiota associated with obesity and disorders of lipid metabolism." @default.
• W4289550045 created "2022-08-03" @default.
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• W4289550045 date "2022-08-03" @default.
• W4289550045 modified "2023-10-17" @default.
• W4289550045 title "Visual Identification of <i>Trichosporon asahii,</i> a Gut Yeast Associated with Obesity, Using an Enzymatic NIR Fluorescent Probe" @default.
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• W4289550045 doi "https://doi.org/10.1021/acs.analchem.2c01691" @default.
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