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- W4289646525 abstract "Toeprint assays are primer extension inhibition assays that can detect the 3' end of an RNA secondary structure, the position of a bound RNA binding protein, as well as the position of a bound 30S ribosomal subunit or a stalled ribosome. Here we describe how this assay was used to identify an RNA hairpin that sequesters a Shine-Dalgarno sequence, how the RNA-binding protein CsrA can alter RNA structure and affect 30S ribosomal subunit binding, and how the macrolide antibiotic tylosin can induce ribosome stalling." @default.
- W4289646525 created "2022-08-03" @default.
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- W4289646525 date "2022-01-01" @default.
- W4289646525 modified "2023-09-26" @default.
- W4289646525 title "Toeprint Assays for Detecting RNA Structure and Protein–RNA Interactions" @default.
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- W4289646525 doi "https://doi.org/10.1007/978-1-0716-2413-5_16" @default.
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