FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4289731296> ?p ?o ?g. }

• W4289731296 abstract "Excess PPi triggers developmental defects in a cell-autonomous manner. The level of inorganic pyrophosphate (PPi) must be tightly regulated in all kingdoms for the proper execution of cellular functions. In plants, the vacuolar proton pyrophosphatase (H + -PPase) has a pivotal role in PPi homeostasis. We previously demonstrated that the excess cytosolic PPi in the H + -PPase loss-of-function fugu5 mutant inhibits gluconeogenesis from seed storage lipids, arrests cell division in cotyledonary palisade tissue, and triggers a compensated cell enlargement (CCE). Moreover, PPi alters pavement cell (PC) shape, stomatal patterning, and functioning, supporting specific yet broad inhibitory effects of PPi on leaf morphogenesis. Whereas these developmental defects were totally rescued by the expression of the yeast soluble pyrophosphatase IPP1, sucrose supply alone canceled CCE in the palisade tissue but not the epidermal developmental defects. Hence, we postulated that the latter are likely triggered by excess PPi rather than a sucrose deficit. To formally test this hypothesis, we adopted a spatiotemporal approach by constructing and analyzing fugu5-1 PDF1 pro ::IPP1 , fugu5-1 CLV1 pro ::IPP1 , and fugu5-1 ICL pro ::IPP1 , whereby PPi was removed specifically from the epidermis, palisade tissue cells, or during the 4 days following seed imbibition, respectively. It is important to note that whereas PC defects in fugu5-1 PDF1 pro ::IPP1 were completely recovered, those in fugu5-1 CLV1 pro ::IPP1 were not. In addition, phenotypic analyses of fugu5-1 ICL pro ::IPP1 lines demonstrated that the immediate removal of PPi after seed imbibition markedly improved overall plant growth, abolished CCE, but only partially restored the epidermal developmental defects. Next, the impact of spatial and temporal removal of PPi was investigated by capillary electrophoresis time-of-flight mass spectrometry (CE-TOF MS). Our analysis revealed that the metabolic profiles are differentially affected among all the above transgenic lines, and consistent with an axial role of central metabolism of gluconeogenesis in CCE. Taken together, this study provides a conceptual framework to unveil metabolic fluctuations within leaf tissues with high spatio–temporal resolution. Finally, our findings suggest that excess PPi exerts its inhibitory effect in planta in the early stages of seedling establishment in a tissue- and cell-autonomous manner." @default.
• W4289731296 created "2022-08-04" @default.
• W4289731296 creator A5011698578 @default.
• W4289731296 creator A5015663350 @default.
• W4289731296 creator A5024140477 @default.
• W4289731296 creator A5024742387 @default.
• W4289731296 creator A5038913847 @default.
• W4289731296 creator A5044540756 @default.
• W4289731296 creator A5064754063 @default.
• W4289731296 creator A5090036035 @default.
• W4289731296 creator A5090406100 @default.
• W4289731296 date "2022-08-04" @default.
• W4289731296 modified "2023-09-30" @default.
• W4289731296 title "Tissue-targeted inorganic pyrophosphate hydrolysis in a fugu5 mutant reveals that excess inorganic pyrophosphate triggers developmental defects in a cell-autonomous manner" @default.
• W4289731296 cites W1495425163 @default.
• W4289731296 cites W1591613693 @default.
• W4289731296 cites W1904214194 @default.
• W4289731296 cites W1911175850 @default.
• W4289731296 cites W1967004003 @default.
• W4289731296 cites W1967418010 @default.
• W4289731296 cites W1969958023 @default.
• W4289731296 cites W1971001847 @default.
• W4289731296 cites W1979264410 @default.
• W4289731296 cites W1990213589 @default.
• W4289731296 cites W1990359266 @default.
• W4289731296 cites W1991463273 @default.
• W4289731296 cites W1995777848 @default.
• W4289731296 cites W1999308700 @default.
• W4289731296 cites W2000854426 @default.
• W4289731296 cites W2005433438 @default.
• W4289731296 cites W2011177067 @default.
• W4289731296 cites W2013534878 @default.
• W4289731296 cites W2014513938 @default.
• W4289731296 cites W2018051595 @default.
• W4289731296 cites W2018507034 @default.
• W4289731296 cites W2019643071 @default.
• W4289731296 cites W2020874630 @default.
• W4289731296 cites W2022527396 @default.
• W4289731296 cites W2024556021 @default.
• W4289731296 cites W2025814329 @default.
• W4289731296 cites W2031798358 @default.
• W4289731296 cites W2032071626 @default.
• W4289731296 cites W2036508527 @default.
• W4289731296 cites W2044362751 @default.
• W4289731296 cites W2053146826 @default.
• W4289731296 cites W2071213711 @default.
• W4289731296 cites W2075833860 @default.
• W4289731296 cites W2077739894 @default.
• W4289731296 cites W2079663203 @default.
• W4289731296 cites W2095974359 @default.
• W4289731296 cites W2104886406 @default.
• W4289731296 cites W2106697551 @default.
• W4289731296 cites W2109980254 @default.
• W4289731296 cites W2114060847 @default.
• W4289731296 cites W2115805551 @default.
• W4289731296 cites W2116122104 @default.
• W4289731296 cites W2116324539 @default.
• W4289731296 cites W2119116806 @default.
• W4289731296 cites W2124021617 @default.
• W4289731296 cites W2129578940 @default.
• W4289731296 cites W2129847122 @default.
• W4289731296 cites W2131601308 @default.
• W4289731296 cites W2132293500 @default.
• W4289731296 cites W2136253578 @default.
• W4289731296 cites W2139491669 @default.
• W4289731296 cites W2141859903 @default.
• W4289731296 cites W2146374071 @default.
• W4289731296 cites W2156238256 @default.
• W4289731296 cites W2156583214 @default.
• W4289731296 cites W2157410227 @default.
• W4289731296 cites W2159170265 @default.
• W4289731296 cites W2159185415 @default.
• W4289731296 cites W2160118949 @default.
• W4289731296 cites W2163930938 @default.
• W4289731296 cites W2165757276 @default.
• W4289731296 cites W2180945455 @default.
• W4289731296 cites W2217377357 @default.
• W4289731296 cites W2295631374 @default.
• W4289731296 cites W2317350971 @default.
• W4289731296 cites W2319986197 @default.
• W4289731296 cites W2414430863 @default.
• W4289731296 cites W2515929567 @default.
• W4289731296 cites W2532578490 @default.
• W4289731296 cites W2563830720 @default.
• W4289731296 cites W2588135909 @default.
• W4289731296 cites W2767523747 @default.
• W4289731296 cites W2788326585 @default.
• W4289731296 cites W2801209353 @default.
• W4289731296 cites W2801682416 @default.
• W4289731296 cites W2888589461 @default.
• W4289731296 cites W2892984827 @default.
• W4289731296 cites W2910589767 @default.
• W4289731296 cites W2964524707 @default.
• W4289731296 cites W2974361058 @default.
• W4289731296 cites W3001672917 @default.
• W4289731296 cites W3008501888 @default.
• W4289731296 cites W3033607206 @default.
• W4289731296 cites W3084208273 @default.
• W4289731296 cites W3127402450 @default.
• W4289731296 cites W3131903870 @default.

• next