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• W4289778894 endingPage "102455" @default.
• W4289778894 startingPage "102455" @default.
• W4289778894 abstract "The aim of this work is to optimize the nutritional conditions using low-cost carbon and nitrogen sources for microsclerotia (MS) production of two Brazilian Trichoderma asperellum strains (BRM-29576 and BRM-29104). A fractional factorial design was used to investigate five variables: carbon source, carbon concentration, nitrogen source, C:N ratio and fungal strain. In this study, the optimal nutritional condition for MS yield was achieved using sucrose and autolyzed yeast, carbon concentration at 20 g L−1 and C:N ratio of 10:1 for the BRM-29104 strain, which yielded up to 2.5 (±0.9) × 104 MS mL−1 within 7 days of fermentation. Sucrose and autolyzed yeast are two economically available carbon and nitrogen sources in Brazil. Vacuum filtration of MS biomass with nylon filter (200 mesh/74 μm) in a plastic funnel increased MS concentration, resulting in up to 1.8 (±0.2) × 105 MS g−1. Our results support a cost-efficient liquid medium to achieve high MS yields by BRM-29104 in combination with an easy method to harvest MS from culture broth for further formulation development." @default.
• W4289778894 created "2022-08-04" @default.
• W4289778894 creator A5001761494 @default.
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• W4289778894 date "2022-09-01" @default.
• W4289778894 modified "2023-09-26" @default.
• W4289778894 title "Production of microsclerotia by Trichoderma asperellum through submerged liquid fermentation using low-cost nitrogen and carbon sources" @default.
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• W4289778894 doi "https://doi.org/10.1016/j.bcab.2022.102455" @default.
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