FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4289784890> ?p ?o ?g. }

• W4289784890 endingPage "11658" @default.
• W4289784890 startingPage "11650" @default.
• W4289784890 abstract "We report a segmented spectrum scan method using Orbitrap MS in chemical isotope labeling (CIL) liquid chromatography–mass spectrometry (LC–MS) for improving the metabolite detection efficiency. In this method, the full m/z range is divided into multiple segments with the scanning of each segment to produce multiple narrow-range spectra during the LC data acquisition. These segmented spectra are separately processed to extract the peak pair information with each peak pair arising from a differentially labeled metabolite in the analysis of a mixture of 13C and 12C reagent-labeled samples. The sublists of peak pairs are merged to form the final peak pair list from the LC–MS run. Various experimental conditions, including automatic gain control (AGC) values, mass resolutions, segment m/z widths, number of segments, and total data acquisition time in the LC run, were examined to arrive at an optimal setting in the segment scan for increasing the number of detectable metabolites while maintaining the same analysis time as in the full scan. The optimal method used a segment width of 120 m/z with 60k resolution for a 16 min CIL LC–MS run. Using dansyl-labeled human urine samples as an example, we demonstrated that this method could detect 5867 peak pairs or metabolites (not features), compared to 3765 peak pairs detectable in a full scan, representing a 56% gain. Out of 5867 peak pairs, 5575 (95.0%) could be identified or mass-matched. The relative quantification accuracy was slightly reduced (81% peak pairs were within ±25% of the expected peak ratio of 1.0 in full, compared to 87% in the full scan) due to the inclusion of more low-abundance peak pairs in the segment scan. The peak ratio measurement precision was not significantly affected by the segment scan. We also showed the increase of the peak pair number detectable from 3843 in the full scan to 7273 (89% gain) using the Orbitrap operated at 120k resolution with a 60 m/z segment width when multiple repeat sample injections were used. Thus, segment scan Orbitrap MS is an enabling method for detecting coeluting metabolites in CIL LC–MS for increasing the metabolomic coverage." @default.
• W4289784890 created "2022-08-04" @default.
• W4289784890 creator A5025315109 @default.
• W4289784890 creator A5044711861 @default.
• W4289784890 date "2022-08-04" @default.
• W4289784890 modified "2023-10-18" @default.
• W4289784890 title "Segment Scan Mass Spectral Acquisition for Increasing the Metabolite Detectability in Chemical Isotope Labeling Liquid Chromatography–Mass Spectrometry Metabolome Analysis" @default.
• W4289784890 cites W2009863844 @default.
• W4289784890 cites W2030392928 @default.
• W4289784890 cites W2095133506 @default.
• W4289784890 cites W2120171195 @default.
• W4289784890 cites W2319708779 @default.
• W4289784890 cites W2321570752 @default.
• W4289784890 cites W2396430205 @default.
• W4289784890 cites W2473466345 @default.
• W4289784890 cites W2533060394 @default.
• W4289784890 cites W2548530122 @default.
• W4289784890 cites W2610435987 @default.
• W4289784890 cites W2761700579 @default.
• W4289784890 cites W2769815973 @default.
• W4289784890 cites W2893993700 @default.
• W4289784890 cites W2963567889 @default.
• W4289784890 cites W2969351161 @default.
• W4289784890 cites W2969909998 @default.
• W4289784890 cites W2982549615 @default.
• W4289784890 cites W3040653733 @default.
• W4289784890 cites W3044577873 @default.
• W4289784890 cites W3114161997 @default.
• W4289784890 cites W3114604754 @default.
• W4289784890 cites W4205550832 @default.
• W4289784890 cites W4221053439 @default.
• W4289784890 doi "https://doi.org/10.1021/acs.analchem.2c02220" @default.
• W4289784890 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35926115" @default.
• W4289784890 hasPublicationYear "2022" @default.
• W4289784890 type Work @default.
• W4289784890 citedByCount "2" @default.
• W4289784890 countsByYear W42897848902023 @default.
• W4289784890 crossrefType "journal-article" @default.
• W4289784890 hasAuthorship W4289784890A5025315109 @default.
• W4289784890 hasAuthorship W4289784890A5044711861 @default.
• W4289784890 hasConcept C113196181 @default.
• W4289784890 hasConcept C121332964 @default.
• W4289784890 hasConcept C127124265 @default.
• W4289784890 hasConcept C135870905 @default.
• W4289784890 hasConcept C138268822 @default.
• W4289784890 hasConcept C154945302 @default.
• W4289784890 hasConcept C162356407 @default.
• W4289784890 hasConcept C164304813 @default.
• W4289784890 hasConcept C178790620 @default.
• W4289784890 hasConcept C185592680 @default.
• W4289784890 hasConcept C21565614 @default.
• W4289784890 hasConcept C2777477808 @default.
• W4289784890 hasConcept C2780841973 @default.
• W4289784890 hasConcept C32909587 @default.
• W4289784890 hasConcept C40325409 @default.
• W4289784890 hasConcept C41008148 @default.
• W4289784890 hasConcept C43617362 @default.
• W4289784890 hasConcept C55493867 @default.
• W4289784890 hasConcept C62520636 @default.
• W4289784890 hasConceptScore W4289784890C113196181 @default.
• W4289784890 hasConceptScore W4289784890C121332964 @default.
• W4289784890 hasConceptScore W4289784890C127124265 @default.
• W4289784890 hasConceptScore W4289784890C135870905 @default.
• W4289784890 hasConceptScore W4289784890C138268822 @default.
• W4289784890 hasConceptScore W4289784890C154945302 @default.
• W4289784890 hasConceptScore W4289784890C162356407 @default.
• W4289784890 hasConceptScore W4289784890C164304813 @default.
• W4289784890 hasConceptScore W4289784890C178790620 @default.
• W4289784890 hasConceptScore W4289784890C185592680 @default.
• W4289784890 hasConceptScore W4289784890C21565614 @default.
• W4289784890 hasConceptScore W4289784890C2777477808 @default.
• W4289784890 hasConceptScore W4289784890C2780841973 @default.
• W4289784890 hasConceptScore W4289784890C32909587 @default.
• W4289784890 hasConceptScore W4289784890C40325409 @default.
• W4289784890 hasConceptScore W4289784890C41008148 @default.
• W4289784890 hasConceptScore W4289784890C43617362 @default.
• W4289784890 hasConceptScore W4289784890C55493867 @default.
• W4289784890 hasConceptScore W4289784890C62520636 @default.
• W4289784890 hasFunder F4320310787 @default.
• W4289784890 hasFunder F4320319952 @default.
• W4289784890 hasFunder F4320334593 @default.
• W4289784890 hasIssue "33" @default.
• W4289784890 hasLocation W42897848901 @default.
• W4289784890 hasLocation W42897848902 @default.
• W4289784890 hasOpenAccess W4289784890 @default.
• W4289784890 hasPrimaryLocation W42897848901 @default.
• W4289784890 hasRelatedWork W2003174742 @default.
• W4289784890 hasRelatedWork W2025071171 @default.
• W4289784890 hasRelatedWork W2081311904 @default.
• W4289784890 hasRelatedWork W2165009222 @default.
• W4289784890 hasRelatedWork W2172264830 @default.
• W4289784890 hasRelatedWork W2321376996 @default.
• W4289784890 hasRelatedWork W2559432842 @default.
• W4289784890 hasRelatedWork W3007443317 @default.
• W4289784890 hasRelatedWork W3013031720 @default.
• W4289784890 hasRelatedWork W4289784890 @default.
• W4289784890 hasVolume "94" @default.
• W4289784890 isParatext "false" @default.

• next