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- W4289793480 abstract "Mycorrhizae are important to plants in improving nutrient absorption and stress resistance. To study mycorrhizal fungal diversity in blueberry, we combined culture method and culture-independent molecular method to analyze the root endosphere and rhizosphere fungi in three different cultivars. We obtained 212 isolates with a culture method and classified them into 40 types according to their morphological characteristics. Then, we amplified the internal transcribed spacer sequence and found rich species diversity. With high-throughput sequencing, 561 operational taxonomic units (OTUs) were annotated based on a 97% similarity level cutoff. The alpha diversity index revealed that the fungal abundance and diversity in the rhizosphere were higher than those in the endosphere. The dominant phyla were Ascomycota and Basidiomycota and the dominant genus was Oidiodendron. We also constructed the plant–fungus symbiotic system by inoculating in vitro stock shoots, which lays a theoretical foundation for further research to develop and utilize the dominant mycorrhizal fungi of blueberry." @default.
- W4289793480 created "2022-08-04" @default.
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- W4289793480 date "2022-10-01" @default.
- W4289793480 modified "2023-09-25" @default.
- W4289793480 title "Diversity of fungal assemblages in rhizosphere and endosphere of blueberry (<i>Vaccinium</i> spp.) under field conditions revealed by culturing and culture-independent molecular methods" @default.
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- W4289793480 doi "https://doi.org/10.1139/cjm-2022-0093" @default.
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