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• W4289831747 endingPage "140830" @default.
• W4289831747 startingPage "140830" @default.
• W4289831747 abstract "Differential scanning calorimetry (DSC) determines the enthalpy change upon protein unfolding and the melting temperature of the protein. Performing DSC of a protein in the presence of increasing concentrations of specifically-binding ligand yields a series of curves that can be fit to obtain the protein-ligand dissociation constant as done in the fluorescence-based thermal shift assay (FTSA, ThermoFluor, DSF). The enthalpy of unfolding, as directly determined by DSC, helps improving the precision of the fit. If the ligand binding is linked to protonation reactions, the intrinsic binding constant can be determined by performing the affinity determination at a series of pH values. Here, the intrinsic, pH-independent, affinity of acetazolamide binding to carbonic anhydrase (CA) II was determined. A series of high-affinity ligands binding to CAIX, an anticancer drug target, and CAII showed recognition and selectivity for the anticancer isozyme. Performing the DSC experiment in buffers of highly different enthalpies of protonation enabled to observe the ligand unbinding-linked protonation reactions and estimate the intrinsic enthalpy of binding. The heat capacity of combined unfolding and unbinding was determined by varying the ligand concentrations. Taken together, these parameters provided a detailed thermodynamic picture of the linked ligand binding and protein unfolding process." @default.
• W4289831747 created "2022-08-05" @default.
• W4289831747 creator A5067082313 @default.
• W4289831747 creator A5069953531 @default.
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• W4289831747 date "2022-09-01" @default.
• W4289831747 modified "2023-09-27" @default.
• W4289831747 title "Intrinsic affinity of protein – ligand binding by differential scanning calorimetry" @default.
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• W4289831747 doi "https://doi.org/10.1016/j.bbapap.2022.140830" @default.
• W4289831747 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35934299" @default.
• W4289831747 hasPublicationYear "2022" @default.
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