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- W4289847506 abstract "Abstract Background Rabies is a neurotropic virus that causes about 59000 deaths worldwide annually. The most effective means to control and prevent rabies is prevention through proper pre- and post-exposure vaccination. Glycoprotein (G) is one of five structural proteins of the rabies virus and has a pivotal role in host immunity against the virus. This research has evaluated the results of incorporating an additional copy of the glycoprotein gene in the rabies virus genome on the immunogenicity and propagation rate of the recombinant virus. Methods . A PCR amplified copy of the G gene was previously inserted into the genome of the rabies virus PV strain. The recombinant virus glycoprotein expression was compared with the PV strain. The propagation rate of the recombinant virus in cell culture and its immunogenicity in BALB/c mice were assessed. The rapid fluorescent focus inhibition test (RFFIT) was used to analyze the virus-neutralizing antibodies (VNAs) in the mice sera. Results The addition of an extra G gene between the G and L genes was verified in the rescued recombinant virus. The virus strain carrying two G (dG) showed significantly higher virus titers and glycoprotein expression levels in cell culture and also induced higher titers of VNAs when applied in mice as an experimental vaccine. Conclusion Our results suggest that duplication of the G gene in the PV virus genome between G, and L genes leads to increased G expression level, higher virus propagation rates and improved VNA induction. The recombinant dG strain might be characterized for application in rabies vaccine production, and it can also be used to study different cellular pathways related to the rabies virus cycle." @default.
- W4289847506 created "2022-08-05" @default.
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- W4289847506 date "2022-08-04" @default.
- W4289847506 modified "2023-10-08" @default.
- W4289847506 title "The Double Glycoprotein Rabies Virus Strain Shows Increased Propagation Rate and Boosted Immunogenicity." @default.
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- W4289847506 doi "https://doi.org/10.21203/rs.3.rs-1911773/v1" @default.
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