FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4289953209> ?p ?o ?g. }

• W4289953209 endingPage "2303" @default.
• W4289953209 startingPage "2296" @default.
• W4289953209 abstract "Peptide epitope tags offer a valuable means for detection and manipulation of protein targets for which high quality detection reagents are not available. Most commonly used epitope tags are bound by conventional, full-size antibodies (Abs). The complex architecture of Abs complicates their application in protein engineering and intracellular applications. To address these shortcomings, single domain antibodies (nanobodies, Nbs) that recognize short peptide epitopes have become increasingly prized. Here, we characterize the interaction between a Nb (Nb6E) and a 14-mer peptide epitope. We identify residues in the peptide epitope essential for high affinity binding. Using this information in combination with computational modeling we propose a mode of interaction between Nb6E and this epitope. We apply this nanobody-epitope pair to augment the potency of a ligand at an engineered adenosine A2A receptor. This characterization of the nanobody-epitope pair opens the door to diverse applications including mechanistic studies of the G protein-coupled receptor function." @default.
• W4289953209 created "2022-08-06" @default.
• W4289953209 creator A5018356508 @default.
• W4289953209 creator A5034227606 @default.
• W4289953209 creator A5045230401 @default.
• W4289953209 date "2022-08-05" @default.
• W4289953209 modified "2023-10-11" @default.
• W4289953209 title "Characterization of a Nanobody-Epitope Tag Interaction and Its Application for Receptor Engineering" @default.
• W4289953209 cites W1973702904 @default.
• W4289953209 cites W1994323576 @default.
• W4289953209 cites W2078041686 @default.
• W4289953209 cites W2121443918 @default.
• W4289953209 cites W2128297456 @default.
• W4289953209 cites W2156257459 @default.
• W4289953209 cites W2165286214 @default.
• W4289953209 cites W2166956176 @default.
• W4289953209 cites W2333223032 @default.
• W4289953209 cites W2336359492 @default.
• W4289953209 cites W2412092132 @default.
• W4289953209 cites W2617973906 @default.
• W4289953209 cites W2774294088 @default.
• W4289953209 cites W2886130158 @default.
• W4289953209 cites W2902933072 @default.
• W4289953209 cites W2927684362 @default.
• W4289953209 cites W2940508523 @default.
• W4289953209 cites W2949201915 @default.
• W4289953209 cites W2965468477 @default.
• W4289953209 cites W2972952141 @default.
• W4289953209 cites W2976500897 @default.
• W4289953209 cites W2997234557 @default.
• W4289953209 cites W3009287579 @default.
• W4289953209 cites W3013770476 @default.
• W4289953209 cites W3014589367 @default.
• W4289953209 cites W3015127072 @default.
• W4289953209 cites W3022893071 @default.
• W4289953209 cites W3081559067 @default.
• W4289953209 cites W3081642245 @default.
• W4289953209 cites W3094177723 @default.
• W4289953209 cites W3132641642 @default.
• W4289953209 cites W3177828909 @default.
• W4289953209 cites W3205837077 @default.
• W4289953209 cites W3208960287 @default.
• W4289953209 cites W4220669594 @default.
• W4289953209 cites W4220887281 @default.
• W4289953209 cites W4223580995 @default.
• W4289953209 cites W4281289153 @default.
• W4289953209 cites W4281790889 @default.
• W4289953209 doi "https://doi.org/10.1021/acschembio.2c00407" @default.
• W4289953209 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35930411" @default.
• W4289953209 hasPublicationYear "2022" @default.
• W4289953209 type Work @default.
• W4289953209 citedByCount "5" @default.
• W4289953209 countsByYear W42899532092022 @default.
• W4289953209 countsByYear W42899532092023 @default.
• W4289953209 crossrefType "journal-article" @default.
• W4289953209 hasAuthorship W4289953209A5018356508 @default.
• W4289953209 hasAuthorship W4289953209A5034227606 @default.
• W4289953209 hasAuthorship W4289953209A5045230401 @default.
• W4289953209 hasBestOaLocation W42899532092 @default.
• W4289953209 hasConcept C159654299 @default.
• W4289953209 hasConcept C185592680 @default.
• W4289953209 hasConcept C186268636 @default.
• W4289953209 hasConcept C195616568 @default.
• W4289953209 hasConcept C2779281246 @default.
• W4289953209 hasConcept C2780632150 @default.
• W4289953209 hasConcept C2781241564 @default.
• W4289953209 hasConcept C54355233 @default.
• W4289953209 hasConcept C55493867 @default.
• W4289953209 hasConcept C70721500 @default.
• W4289953209 hasConcept C78598654 @default.
• W4289953209 hasConcept C86803240 @default.
• W4289953209 hasConcept C9365378 @default.
• W4289953209 hasConceptScore W4289953209C159654299 @default.
• W4289953209 hasConceptScore W4289953209C185592680 @default.
• W4289953209 hasConceptScore W4289953209C186268636 @default.
• W4289953209 hasConceptScore W4289953209C195616568 @default.
• W4289953209 hasConceptScore W4289953209C2779281246 @default.
• W4289953209 hasConceptScore W4289953209C2780632150 @default.
• W4289953209 hasConceptScore W4289953209C2781241564 @default.
• W4289953209 hasConceptScore W4289953209C54355233 @default.
• W4289953209 hasConceptScore W4289953209C55493867 @default.
• W4289953209 hasConceptScore W4289953209C70721500 @default.
• W4289953209 hasConceptScore W4289953209C78598654 @default.
• W4289953209 hasConceptScore W4289953209C86803240 @default.
• W4289953209 hasConceptScore W4289953209C9365378 @default.
• W4289953209 hasFunder F4320332161 @default.
• W4289953209 hasFunder F4320337357 @default.
• W4289953209 hasIssue "8" @default.
• W4289953209 hasLocation W42899532091 @default.
• W4289953209 hasLocation W42899532092 @default.
• W4289953209 hasLocation W42899532093 @default.
• W4289953209 hasLocation W42899532094 @default.
• W4289953209 hasOpenAccess W4289953209 @default.
• W4289953209 hasPrimaryLocation W42899532091 @default.
• W4289953209 hasRelatedWork W2076628539 @default.
• W4289953209 hasRelatedWork W2141316307 @default.
• W4289953209 hasRelatedWork W2242496419 @default.
• W4289953209 hasRelatedWork W2361799422 @default.

• next