Matches in SemOpenAlex for { <https://semopenalex.org/work/W4290005681> ?p ?o ?g. }
- W4290005681 abstract "Abstract Extended depth of field (EDoF) microscopy has emerged as a powerful solution to greatly increase the access into neuronal populations in table-top imaging platforms. Here, we present EDoF-Miniscope, which integrates an optimized thin and lightweight binary diffractive optical element (DOE) onto the gradient refractive index (GRIN) lens of a head-mounted fluorescence miniature microscope, i.e. “miniscope”. We achieve an alignment accuracy of 70 μm to allow a 2.8X depth-of-field extension between the twin foci. We optimize the phase profile across the whole back aperture through a genetic algorithm that considers the primary GRIN lens aberrations, optical property of the submersion media, and axial intensity loss from tissue scattering in a Fourier optics forward model. Compared to other computational miniscopes, our EDoF-Miniscope produces high-contrast signals that can be recovered by a simple algorithm and can successfully capture volumetrically distributed neuronal signals without significantly compromising the speed, signal-to-noise, signal-to-background, and maintain a comparable 0.9-μm lateral spatial resolution and the size and weight of the miniature platform. We demonstrate the robustness of EDoF-Miniscope against scattering by characterizing its performance in 5-μm and 10-μm beads embedded in scattering phantoms. We demonstrate that EDoF-Miniscope facilitates deeper interrogations of neuronal populations in a 100-μm thick mouse brain sample, as well as vessels in a mouse brain. Built from off-the-shelf components augmented by a customizable DOE, we expect that this low-cost EDoF-Miniscope may find utility in a wide range of neural recording applications." @default.
- W4290005681 created "2022-08-06" @default.
- W4290005681 creator A5001659250 @default.
- W4290005681 creator A5012281697 @default.
- W4290005681 creator A5013887346 @default.
- W4290005681 creator A5020594160 @default.
- W4290005681 creator A5021316989 @default.
- W4290005681 creator A5027397372 @default.
- W4290005681 creator A5034369710 @default.
- W4290005681 creator A5082957688 @default.
- W4290005681 date "2022-08-05" @default.
- W4290005681 modified "2023-10-18" @default.
- W4290005681 title "EDoF-Miniscope: pupil engineering for extended depth-of-field imaging in a fluorescence miniscope" @default.
- W4290005681 cites W1970157627 @default.
- W4290005681 cites W1976654328 @default.
- W4290005681 cites W1983686562 @default.
- W4290005681 cites W2083461999 @default.
- W4290005681 cites W2098681947 @default.
- W4290005681 cites W2169314018 @default.
- W4290005681 cites W2404947714 @default.
- W4290005681 cites W2616708953 @default.
- W4290005681 cites W2751815772 @default.
- W4290005681 cites W2769713965 @default.
- W4290005681 cites W2800089816 @default.
- W4290005681 cites W2904641582 @default.
- W4290005681 cites W2907402204 @default.
- W4290005681 cites W2930741432 @default.
- W4290005681 cites W2979942150 @default.
- W4290005681 cites W2989972355 @default.
- W4290005681 cites W3000396208 @default.
- W4290005681 cites W3006617518 @default.
- W4290005681 cites W3007508039 @default.
- W4290005681 cites W3028393458 @default.
- W4290005681 cites W3035479716 @default.
- W4290005681 cites W3092329722 @default.
- W4290005681 cites W3094427760 @default.
- W4290005681 cites W3095194336 @default.
- W4290005681 cites W3104608919 @default.
- W4290005681 cites W3112598205 @default.
- W4290005681 cites W3130916464 @default.
- W4290005681 cites W3134482516 @default.
- W4290005681 cites W3164365580 @default.
- W4290005681 cites W3175585329 @default.
- W4290005681 cites W3207362856 @default.
- W4290005681 cites W4200160233 @default.
- W4290005681 cites W4200249624 @default.
- W4290005681 cites W4220743323 @default.
- W4290005681 cites W4220899191 @default.
- W4290005681 cites W4221025960 @default.
- W4290005681 cites W4229336670 @default.
- W4290005681 doi "https://doi.org/10.1101/2022.08.05.502947" @default.
- W4290005681 hasPublicationYear "2022" @default.
- W4290005681 type Work @default.
- W4290005681 citedByCount "1" @default.
- W4290005681 countsByYear W42900056812023 @default.
- W4290005681 crossrefType "posted-content" @default.
- W4290005681 hasAuthorship W4290005681A5001659250 @default.
- W4290005681 hasAuthorship W4290005681A5012281697 @default.
- W4290005681 hasAuthorship W4290005681A5013887346 @default.
- W4290005681 hasAuthorship W4290005681A5020594160 @default.
- W4290005681 hasAuthorship W4290005681A5021316989 @default.
- W4290005681 hasAuthorship W4290005681A5027397372 @default.
- W4290005681 hasAuthorship W4290005681A5034369710 @default.
- W4290005681 hasAuthorship W4290005681A5082957688 @default.
- W4290005681 hasBestOaLocation W42900056811 @default.
- W4290005681 hasConcept C120665830 @default.
- W4290005681 hasConcept C121332964 @default.
- W4290005681 hasConcept C147080431 @default.
- W4290005681 hasConcept C15336307 @default.
- W4290005681 hasConcept C159108749 @default.
- W4290005681 hasConcept C183072630 @default.
- W4290005681 hasConcept C191486275 @default.
- W4290005681 hasConcept C192562407 @default.
- W4290005681 hasConcept C6260449 @default.
- W4290005681 hasConcept C67649825 @default.
- W4290005681 hasConceptScore W4290005681C120665830 @default.
- W4290005681 hasConceptScore W4290005681C121332964 @default.
- W4290005681 hasConceptScore W4290005681C147080431 @default.
- W4290005681 hasConceptScore W4290005681C15336307 @default.
- W4290005681 hasConceptScore W4290005681C159108749 @default.
- W4290005681 hasConceptScore W4290005681C183072630 @default.
- W4290005681 hasConceptScore W4290005681C191486275 @default.
- W4290005681 hasConceptScore W4290005681C192562407 @default.
- W4290005681 hasConceptScore W4290005681C6260449 @default.
- W4290005681 hasConceptScore W4290005681C67649825 @default.
- W4290005681 hasLocation W42900056811 @default.
- W4290005681 hasOpenAccess W4290005681 @default.
- W4290005681 hasPrimaryLocation W42900056811 @default.
- W4290005681 hasRelatedWork W1612930626 @default.
- W4290005681 hasRelatedWork W1996093649 @default.
- W4290005681 hasRelatedWork W2025604929 @default.
- W4290005681 hasRelatedWork W2058964314 @default.
- W4290005681 hasRelatedWork W2326867374 @default.
- W4290005681 hasRelatedWork W2403466521 @default.
- W4290005681 hasRelatedWork W2435282358 @default.
- W4290005681 hasRelatedWork W2732776331 @default.
- W4290005681 hasRelatedWork W3039786571 @default.
- W4290005681 hasRelatedWork W3165667395 @default.
- W4290005681 isParatext "false" @default.
- W4290005681 isRetracted "false" @default.