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- W4290300147 abstract "The crude venom of spider Latrodectus mactans tredecimguttatus was fractionated by the combination of anion exchange and hydrophobic chromatography. The biological activity of fraction was tested by means of: 1) estimation of toxicity for housefly larva; 2) intracellular recording of miniature excitatory potentials (MEPSPs) in blowfly larvae muscle fibres. As a result of sequential procedures of chromatography separation a homogeneous protein of 120 kilodalton molecular weight was obtained. This protein referred to alpha-latroinsectotoxin produced: 1) a great increase of the frequency of MEPSPs in the dose of 4.2.10(-10) M and its paralytic dose for fly larva was approximately 20 ng/species; 2) no influence of the MEPSPs after application in the dose of 1.2.10(-7) M to the neuromuscular junction of the frog." @default.
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- W4290300147 date "1990-08-01" @default.
- W4290300147 modified "2023-09-29" @default.
- W4290300147 title "[Identification and isolation of the protein insect toxin (alpha-latroinsectotoxin from venom of the spider Latrodectus mactans tredecimguttatus]." @default.
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