FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4290466614> ?p ?o ?g. }

• W4290466614 endingPage "2422" @default.
• W4290466614 startingPage "2422" @default.
• W4290466614 abstract "Background: Chronic Obstructive Pulmonary Disease (COPD), a major cause of mortality and disability, is a complex disease with heterogeneous and ill-understood biological mechanisms. Human induced pluripotent stem cells (hiPSCs) are a promising tool to model human disease, including the impact of genetic susceptibility. Methods: We developed a simple and reliable method for reprogramming peripheral blood mononuclear cells into hiPSCs and to differentiate them into air−liquid interface bronchial epithelium within 45 days. Importantly, this method does not involve any cell sorting step. We reprogrammed blood cells from one healthy control and three patients with very severe COPD. Results: The mean cell purity at the definitive endoderm and ventral anterior foregut endoderm (vAFE) stages was >80%, assessed by quantifying C-X-C Motif Chemokine Receptor 4/SRY-Box Transcription Factor 17 (CXCR4/SOX17) and NK2 Homeobox 1 (NKX2.1) expression, respectively. vAFE cells from all four hiPSC lines differentiated into bronchial epithelium in air−liquid interface conditions, with large zones covered by beating ciliated, basal, goblets, club cells and neuroendocrine cells, as found in vivo. The hiPSC-derived airway epithelium (iALI) from patients with very severe COPD and from the healthy control were undistinguishable. Conclusions: iALI bronchial epithelium is ready for better understanding lung disease pathogenesis and accelerating drug discovery." @default.
• W4290466614 created "2022-08-08" @default.
• W4290466614 creator A5000900684 @default.
• W4290466614 creator A5016678467 @default.
• W4290466614 creator A5036173989 @default.
• W4290466614 creator A5038824744 @default.
• W4290466614 creator A5043972952 @default.
• W4290466614 creator A5044177926 @default.
• W4290466614 creator A5045456582 @default.
• W4290466614 creator A5051134222 @default.
• W4290466614 creator A5051689782 @default.
• W4290466614 creator A5066991759 @default.
• W4290466614 creator A5073032250 @default.
• W4290466614 creator A5076781650 @default.
• W4290466614 creator A5085684624 @default.
• W4290466614 creator A5090527489 @default.
• W4290466614 creator A5091246104 @default.
• W4290466614 date "2022-08-05" @default.
• W4290466614 modified "2023-09-25" @default.
• W4290466614 title "Differentiation of Human Induced Pluripotent Stem Cells from Patients with Severe COPD into Functional Airway Epithelium" @default.
• W4290466614 cites W1859206056 @default.
• W4290466614 cites W2003211588 @default.
• W4290466614 cites W2032678043 @default.
• W4290466614 cites W2049462154 @default.
• W4290466614 cites W2052350776 @default.
• W4290466614 cites W2076018642 @default.
• W4290466614 cites W2076312582 @default.
• W4290466614 cites W2108160744 @default.
• W4290466614 cites W2121459729 @default.
• W4290466614 cites W2127479534 @default.
• W4290466614 cites W2132020845 @default.
• W4290466614 cites W2140859057 @default.
• W4290466614 cites W2145466675 @default.
• W4290466614 cites W2156862506 @default.
• W4290466614 cites W2169862468 @default.
• W4290466614 cites W2209535620 @default.
• W4290466614 cites W2231950910 @default.
• W4290466614 cites W2316925809 @default.
• W4290466614 cites W2338392367 @default.
• W4290466614 cites W2598899113 @default.
• W4290466614 cites W2609587333 @default.
• W4290466614 cites W2611660641 @default.
• W4290466614 cites W2750856487 @default.
• W4290466614 cites W2763080178 @default.
• W4290466614 cites W2896532574 @default.
• W4290466614 cites W2899736836 @default.
• W4290466614 cites W2927018258 @default.
• W4290466614 cites W2953029274 @default.
• W4290466614 cites W2996855164 @default.
• W4290466614 cites W3004379083 @default.
• W4290466614 cites W3005995130 @default.
• W4290466614 cites W3089641406 @default.
• W4290466614 cites W3092290197 @default.
• W4290466614 cites W3093620357 @default.
• W4290466614 cites W3204166380 @default.
• W4290466614 cites W3207458967 @default.
• W4290466614 cites W4221112967 @default.
• W4290466614 cites W4223541162 @default.
• W4290466614 cites W6678017 @default.
• W4290466614 cites W2893348136 @default.
• W4290466614 doi "https://doi.org/10.3390/cells11152422" @default.
• W4290466614 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35954266" @default.
• W4290466614 hasPublicationYear "2022" @default.
• W4290466614 type Work @default.
• W4290466614 citedByCount "4" @default.
• W4290466614 countsByYear W42904666142023 @default.
• W4290466614 crossrefType "journal-article" @default.
• W4290466614 hasAuthorship W4290466614A5000900684 @default.
• W4290466614 hasAuthorship W4290466614A5016678467 @default.
• W4290466614 hasAuthorship W4290466614A5036173989 @default.
• W4290466614 hasAuthorship W4290466614A5038824744 @default.
• W4290466614 hasAuthorship W4290466614A5043972952 @default.
• W4290466614 hasAuthorship W4290466614A5044177926 @default.
• W4290466614 hasAuthorship W4290466614A5045456582 @default.
• W4290466614 hasAuthorship W4290466614A5051134222 @default.
• W4290466614 hasAuthorship W4290466614A5051689782 @default.
• W4290466614 hasAuthorship W4290466614A5066991759 @default.
• W4290466614 hasAuthorship W4290466614A5073032250 @default.
• W4290466614 hasAuthorship W4290466614A5076781650 @default.
• W4290466614 hasAuthorship W4290466614A5085684624 @default.
• W4290466614 hasAuthorship W4290466614A5090527489 @default.
• W4290466614 hasAuthorship W4290466614A5091246104 @default.
• W4290466614 hasBestOaLocation W42904666141 @default.
• W4290466614 hasConcept C104317684 @default.
• W4290466614 hasConcept C105702510 @default.
• W4290466614 hasConcept C107459253 @default.
• W4290466614 hasConcept C142724271 @default.
• W4290466614 hasConcept C145103041 @default.
• W4290466614 hasConcept C148738053 @default.
• W4290466614 hasConcept C1491633281 @default.
• W4290466614 hasConcept C170774537 @default.
• W4290466614 hasConcept C18155467 @default.
• W4290466614 hasConcept C203014093 @default.
• W4290466614 hasConcept C2777164004 @default.
• W4290466614 hasConcept C28328180 @default.
• W4290466614 hasConcept C529295009 @default.
• W4290466614 hasConcept C54355233 @default.
• W4290466614 hasConcept C71924100 @default.

• next