FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4290667683> ?p ?o ?g. }

• W4290667683 endingPage "6015" @default.
• W4290667683 startingPage "6007" @default.
• W4290667683 abstract "Probing the kinetics of ligand binding to biomolecules is of paramount interest in biology and pharmacology. Measurements of such kinetic processes provide information on the rate-determining steps that control the binding affinity of ligands to biomolecules, thereby predicting the mechanism of the molecular interaction. In this context, ligand binding to G-quadruplex DNA (GqDNA) structures has attracted tremendous attention primarily because of their use in possible anticancer therapy. Although a large number of G-quadruplex-specific ligands have been proposed, probing the kinetics of G-tetrad-selective binding of (multiple) ligands within a G-quadruplex DNA (GqDNA) structure remains challenging. Most of the earlier studies focused on the thermodynamics of ligand binding; however, the kinetics of ligand association and dissociation with GqDNA, particularly binding of multiple ligands within a GqDNA structure, have not been explored. Here, we propose a simple fluorescence correlation spectroscopy-based method that measures the G-tetrad-selective association and dissociation rates of ligands within a GqDNA structure by correlating the fluorescence fluctuations of a site-specific (5' or 3' end-labeled) fluorophore (Cy3) in GqDNA due to quenching of Cy3 fluorescence, induced by the ligand binding to the G-tetrads. We show that well-known GqDNA ligands, BRACO19, TMPyP4, Hoechst 33258, and Hoechst 33342, have G-tetrad-selective association and dissociation rates, which suggest site-dependent variation of free energy barriers for binding/unbinding of the ligands with GqDNA. We also show that the measured kinetic rates depend not only on the G-tetrad site (5' vs 3' end) but also on the ligand and GqDNA structures." @default.
• W4290667683 created "2022-08-08" @default.
• W4290667683 creator A5001998891 @default.
• W4290667683 creator A5003561176 @default.
• W4290667683 date "2022-08-08" @default.
• W4290667683 modified "2023-10-12" @default.
• W4290667683 title "G-Tetrad-Selective Ligand Binding Kinetics in G-Quadruplex DNA Probed with Fluorescence Correlation Spectroscopy" @default.
• W4290667683 cites W1887262817 @default.
• W4290667683 cites W1967345725 @default.
• W4290667683 cites W1969073916 @default.
• W4290667683 cites W1976145400 @default.
• W4290667683 cites W1981765336 @default.
• W4290667683 cites W1991411602 @default.
• W4290667683 cites W2007634776 @default.
• W4290667683 cites W2016431119 @default.
• W4290667683 cites W2026761200 @default.
• W4290667683 cites W2035030010 @default.
• W4290667683 cites W2046187908 @default.
• W4290667683 cites W2046224550 @default.
• W4290667683 cites W2050913592 @default.
• W4290667683 cites W2055100067 @default.
• W4290667683 cites W2061948699 @default.
• W4290667683 cites W2072631774 @default.
• W4290667683 cites W2083443948 @default.
• W4290667683 cites W2089598395 @default.
• W4290667683 cites W2103412754 @default.
• W4290667683 cites W2109752088 @default.
• W4290667683 cites W2124582915 @default.
• W4290667683 cites W2140590042 @default.
• W4290667683 cites W2140599637 @default.
• W4290667683 cites W2150788604 @default.
• W4290667683 cites W2235657766 @default.
• W4290667683 cites W2252936797 @default.
• W4290667683 cites W2268480512 @default.
• W4290667683 cites W2278505131 @default.
• W4290667683 cites W2313736620 @default.
• W4290667683 cites W2316043787 @default.
• W4290667683 cites W2324370391 @default.
• W4290667683 cites W2334712694 @default.
• W4290667683 cites W2406246263 @default.
• W4290667683 cites W2481448223 @default.
• W4290667683 cites W2513775856 @default.
• W4290667683 cites W2588267243 @default.
• W4290667683 cites W2591101673 @default.
• W4290667683 cites W2784819782 @default.
• W4290667683 cites W2790747858 @default.
• W4290667683 cites W2894616442 @default.
• W4290667683 cites W2913120485 @default.
• W4290667683 cites W2913753776 @default.
• W4290667683 cites W2915198753 @default.
• W4290667683 cites W2932166343 @default.
• W4290667683 cites W2954390038 @default.
• W4290667683 cites W2973721774 @default.
• W4290667683 cites W2999469162 @default.
• W4290667683 cites W3023800615 @default.
• W4290667683 cites W3042522573 @default.
• W4290667683 cites W3090513030 @default.
• W4290667683 cites W3127117064 @default.
• W4290667683 cites W3191490090 @default.
• W4290667683 cites W4206953568 @default.
• W4290667683 cites W4212849951 @default.
• W4290667683 cites W4213330678 @default.
• W4290667683 cites W4282934289 @default.
• W4290667683 cites W599251412 @default.
• W4290667683 doi "https://doi.org/10.1021/acs.jpcb.2c04181" @default.
• W4290667683 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35939531" @default.
• W4290667683 hasPublicationYear "2022" @default.
• W4290667683 type Work @default.
• W4290667683 citedByCount "6" @default.
• W4290667683 countsByYear W42906676832022 @default.
• W4290667683 countsByYear W42906676832023 @default.
• W4290667683 crossrefType "journal-article" @default.
• W4290667683 hasAuthorship W4290667683A5001998891 @default.
• W4290667683 hasAuthorship W4290667683A5003561176 @default.
• W4290667683 hasConcept C102931765 @default.
• W4290667683 hasConcept C111357860 @default.
• W4290667683 hasConcept C116569031 @default.
• W4290667683 hasConcept C121332964 @default.
• W4290667683 hasConcept C12554922 @default.
• W4290667683 hasConcept C144618238 @default.
• W4290667683 hasConcept C147727578 @default.
• W4290667683 hasConcept C147789679 @default.
• W4290667683 hasConcept C148898269 @default.
• W4290667683 hasConcept C152900378 @default.
• W4290667683 hasConcept C158711907 @default.
• W4290667683 hasConcept C170493617 @default.
• W4290667683 hasConcept C178790620 @default.
• W4290667683 hasConcept C185592680 @default.
• W4290667683 hasConcept C2778617687 @default.
• W4290667683 hasConcept C32909587 @default.
• W4290667683 hasConcept C41625074 @default.
• W4290667683 hasConcept C55493867 @default.
• W4290667683 hasConcept C59822182 @default.
• W4290667683 hasConcept C62520636 @default.
• W4290667683 hasConcept C71240020 @default.
• W4290667683 hasConcept C86803240 @default.
• W4290667683 hasConcept C91881484 @default.
• W4290667683 hasConceptScore W4290667683C102931765 @default.

• next