FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4290963887> ?p ?o ?g. }

• W4290963887 endingPage "130224" @default.
• W4290963887 startingPage "130224" @default.
• W4290963887 abstract "Sodium taurocholate co-transportering polypeptide (NTCP, SLC10A1) is a vital bile acid transporter and the functional receptor of hepatitis B and D virus. The oligomerization of NTCP is important for the structural study of its interaction with HBV preS1 peptide.Recombinant NTCPs were expressed in Sf9 host cell using baculoviruses. Function of recombinant NTCP was verified by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS) method. A quantitative fluorescence resonance energy transfer (FRET) method was established to analyze the interaction between NTCP wild type (WT) and mutants. Co-immunoprecipitation (Co-IP) was used to test the interaction between NTCP variants.Sub-cellular location of recombinant NTCPs varies with the modification of NTCP. Bands of monomer, dimer and oligomers were shown in gel analysis of NTCP. Significant FRET was observed between cyan florescence protein (CFP) tagged NTCP and yellow florescence protein (YFP) tagged NTCP. FRET efficiency between CFP- and YFP-NTCP S267F mutants was lower than WT. Co-IP results showed that S267F interacts with WT NTCP when co-expressed in cell.Dimer is the predominant form of NTCP expressed in Sf9 when solubilized with detergent. FRET and Co-IP analysis support that NTCP forms oligomers in Sf9 cell.Our results showed that NTCP formed oligomers in Sf9 cell. Meanwhile the FRET analysis of NTCP variants further elucidated the molecular mechanism of NTCP oligomerization." @default.
• W4290963887 created "2022-08-13" @default.
• W4290963887 creator A5003569116 @default.
• W4290963887 creator A5006575296 @default.
• W4290963887 creator A5049510575 @default.
• W4290963887 creator A5058483819 @default.
• W4290963887 creator A5079594267 @default.
• W4290963887 date "2022-11-01" @default.
• W4290963887 modified "2023-10-13" @default.
• W4290963887 title "Oligomerization of the HBV/HDV functional receptor NTCP expressed in Sf9 insect cell" @default.
• W4290963887 cites W1559776411 @default.
• W4290963887 cites W1634269557 @default.
• W4290963887 cites W1964471078 @default.
• W4290963887 cites W1974999149 @default.
• W4290963887 cites W1997270466 @default.
• W4290963887 cites W2005588447 @default.
• W4290963887 cites W2070530165 @default.
• W4290963887 cites W2099178578 @default.
• W4290963887 cites W2108571956 @default.
• W4290963887 cites W2120426460 @default.
• W4290963887 cites W2123781724 @default.
• W4290963887 cites W2137661705 @default.
• W4290963887 cites W2161455013 @default.
• W4290963887 cites W2164978829 @default.
• W4290963887 cites W2191975304 @default.
• W4290963887 cites W2338985227 @default.
• W4290963887 cites W2549227981 @default.
• W4290963887 cites W2582336434 @default.
• W4290963887 cites W2767255832 @default.
• W4290963887 cites W2883070373 @default.
• W4290963887 cites W2904912734 @default.
• W4290963887 cites W2912500533 @default.
• W4290963887 cites W2933300836 @default.
• W4290963887 cites W2953705431 @default.
• W4290963887 cites W3048111687 @default.
• W4290963887 cites W3049564470 @default.
• W4290963887 cites W3078821236 @default.
• W4290963887 cites W3101539570 @default.
• W4290963887 cites W3103872670 @default.
• W4290963887 cites W3111468556 @default.
• W4290963887 cites W3114735401 @default.
• W4290963887 cites W31290776 @default.
• W4290963887 cites W3175497714 @default.
• W4290963887 cites W3186617101 @default.
• W4290963887 cites W4210753796 @default.
• W4290963887 cites W4280519745 @default.
• W4290963887 cites W4280560666 @default.
• W4290963887 cites W4280567858 @default.
• W4290963887 doi "https://doi.org/10.1016/j.bbagen.2022.130224" @default.
• W4290963887 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35944837" @default.
• W4290963887 hasPublicationYear "2022" @default.
• W4290963887 type Work @default.
• W4290963887 citedByCount "3" @default.
• W4290963887 countsByYear W42909638872023 @default.
• W4290963887 crossrefType "journal-article" @default.
• W4290963887 hasAuthorship W4290963887A5003569116 @default.
• W4290963887 hasAuthorship W4290963887A5006575296 @default.
• W4290963887 hasAuthorship W4290963887A5049510575 @default.
• W4290963887 hasAuthorship W4290963887A5058483819 @default.
• W4290963887 hasAuthorship W4290963887A5079594267 @default.
• W4290963887 hasConcept C103878061 @default.
• W4290963887 hasConcept C104317684 @default.
• W4290963887 hasConcept C121332964 @default.
• W4290963887 hasConcept C12554922 @default.
• W4290963887 hasConcept C143065580 @default.
• W4290963887 hasConcept C153911025 @default.
• W4290963887 hasConcept C170493617 @default.
• W4290963887 hasConcept C174510640 @default.
• W4290963887 hasConcept C185592680 @default.
• W4290963887 hasConcept C207583985 @default.
• W4290963887 hasConcept C2777773057 @default.
• W4290963887 hasConcept C40767141 @default.
• W4290963887 hasConcept C55493867 @default.
• W4290963887 hasConcept C62520636 @default.
• W4290963887 hasConcept C71829478 @default.
• W4290963887 hasConcept C86803240 @default.
• W4290963887 hasConcept C91881484 @default.
• W4290963887 hasConcept C96305047 @default.
• W4290963887 hasConceptScore W4290963887C103878061 @default.
• W4290963887 hasConceptScore W4290963887C104317684 @default.
• W4290963887 hasConceptScore W4290963887C121332964 @default.
• W4290963887 hasConceptScore W4290963887C12554922 @default.
• W4290963887 hasConceptScore W4290963887C143065580 @default.
• W4290963887 hasConceptScore W4290963887C153911025 @default.
• W4290963887 hasConceptScore W4290963887C170493617 @default.
• W4290963887 hasConceptScore W4290963887C174510640 @default.
• W4290963887 hasConceptScore W4290963887C185592680 @default.
• W4290963887 hasConceptScore W4290963887C207583985 @default.
• W4290963887 hasConceptScore W4290963887C2777773057 @default.
• W4290963887 hasConceptScore W4290963887C40767141 @default.
• W4290963887 hasConceptScore W4290963887C55493867 @default.
• W4290963887 hasConceptScore W4290963887C62520636 @default.
• W4290963887 hasConceptScore W4290963887C71829478 @default.
• W4290963887 hasConceptScore W4290963887C86803240 @default.
• W4290963887 hasConceptScore W4290963887C91881484 @default.
• W4290963887 hasConceptScore W4290963887C96305047 @default.
• W4290963887 hasFunder F4320321001 @default.
• W4290963887 hasFunder F4320322927 @default.

• next