FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4290973605> ?p ?o ?g. }

• W4290973605 endingPage "2978" @default.
• W4290973605 startingPage "2969" @default.
• W4290973605 abstract "Cellular translation is responsible for the synthesis of proteins, a highly diverse class of macromolecules that form the basis of biological function. In Escherichia coli, harnessing and engineering of the biomolecular components of translation, such as ribosomes, transfer RNAs (tRNAs), and aminoacyl-tRNA synthetases, has led to both biotechnology products and an expanded genetic code. However, the engineering potential of molecular translation is hampered by the limited capabilities for rapidly sampling the large genomic space necessary to evolve well-coordinated synthetic translation networks inside cells. To address this limitation, we developed a genome engineering method inspired by the action of mobile genetic elements termed mobilization. Mobilization utilizes the stochastic action of the recombinase flippase (FLP) to generate up to ∼400 million genomic insertions, deletions, or rearrangements at flippase recognition target sites per milliliter of culture per OD in living E. coli cells. As a model, we applied our approach to evolve faster-growing E. coli strains living exclusively off genomically expressed tethered ribosomes. In an iterative “pulse-passaging scheme,” we generated genomic libraries of cells via induction of FLP recombinase (pulse) followed by passaging the population without induction of FLP to enrich the resulting population for cells with higher fitness. We observed large structural genomic diversity across these cells, with the fastest growing strains exhibiting a 71% increase in growth rate compared to the ancestral strain. We anticipate that both these strains and the mobilization method will be useful tools for synthetic biology efforts to engineer translation systems." @default.
• W4290973605 created "2022-08-13" @default.
• W4290973605 creator A5015598503 @default.
• W4290973605 creator A5059800162 @default.
• W4290973605 date "2022-08-11" @default.
• W4290973605 modified "2023-10-03" @default.
• W4290973605 title "Mobile Translation Systems Generate Genomically Engineered <i>Escherichia coli</i> Cells with Improved Growth Phenotypes" @default.
• W4290973605 cites W1989460113 @default.
• W4290973605 cites W1994640766 @default.
• W4290973605 cites W2005700846 @default.
• W4290973605 cites W2032129634 @default.
• W4290973605 cites W2051556659 @default.
• W4290973605 cites W2074933455 @default.
• W4290973605 cites W2110069436 @default.
• W4290973605 cites W2114850508 @default.
• W4290973605 cites W2116137883 @default.
• W4290973605 cites W2144779133 @default.
• W4290973605 cites W2159769705 @default.
• W4290973605 cites W2162778008 @default.
• W4290973605 cites W2163266294 @default.
• W4290973605 cites W2165972561 @default.
• W4290973605 cites W2222228083 @default.
• W4290973605 cites W2272368254 @default.
• W4290973605 cites W2290768068 @default.
• W4290973605 cites W2341364730 @default.
• W4290973605 cites W2533711759 @default.
• W4290973605 cites W2561421588 @default.
• W4290973605 cites W2763278454 @default.
• W4290973605 cites W2787886853 @default.
• W4290973605 cites W2792491353 @default.
• W4290973605 cites W2803336135 @default.
• W4290973605 cites W2804640708 @default.
• W4290973605 cites W2891469382 @default.
• W4290973605 cites W2904734978 @default.
• W4290973605 cites W2910080195 @default.
• W4290973605 cites W2915304621 @default.
• W4290973605 cites W2941806633 @default.
• W4290973605 cites W2944969418 @default.
• W4290973605 cites W2969527868 @default.
• W4290973605 cites W2971002155 @default.
• W4290973605 cites W2984786470 @default.
• W4290973605 cites W2984808762 @default.
• W4290973605 cites W2990461950 @default.
• W4290973605 cites W3009230670 @default.
• W4290973605 cites W3016820202 @default.
• W4290973605 cites W3018541891 @default.
• W4290973605 cites W3080645393 @default.
• W4290973605 cites W3081080495 @default.
• W4290973605 cites W3121552485 @default.
• W4290973605 cites W3165839825 @default.
• W4290973605 cites W3171818102 @default.
• W4290973605 cites W4223470765 @default.
• W4290973605 cites W4285387776 @default.
• W4290973605 cites W914903399 @default.
• W4290973605 doi "https://doi.org/10.1021/acssynbio.2c00099" @default.
• W4290973605 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35951371" @default.
• W4290973605 hasPublicationYear "2022" @default.
• W4290973605 type Work @default.
• W4290973605 citedByCount "1" @default.
• W4290973605 countsByYear W42909736052023 @default.
• W4290973605 crossrefType "journal-article" @default.
• W4290973605 hasAuthorship W4290973605A5015598503 @default.
• W4290973605 hasAuthorship W4290973605A5059800162 @default.
• W4290973605 hasBestOaLocation W42909736052 @default.
• W4290973605 hasConcept C102414288 @default.
• W4290973605 hasConcept C104317684 @default.
• W4290973605 hasConcept C105580179 @default.
• W4290973605 hasConcept C141231307 @default.
• W4290973605 hasConcept C144024400 @default.
• W4290973605 hasConcept C149364088 @default.
• W4290973605 hasConcept C149923435 @default.
• W4290973605 hasConcept C156695909 @default.
• W4290973605 hasConcept C191908910 @default.
• W4290973605 hasConcept C2777019660 @default.
• W4290973605 hasConcept C2778918659 @default.
• W4290973605 hasConcept C2779637612 @default.
• W4290973605 hasConcept C2908647359 @default.
• W4290973605 hasConcept C41625074 @default.
• W4290973605 hasConcept C54355233 @default.
• W4290973605 hasConcept C547475151 @default.
• W4290973605 hasConcept C6350086 @default.
• W4290973605 hasConcept C67705224 @default.
• W4290973605 hasConcept C70721500 @default.
• W4290973605 hasConcept C86803240 @default.
• W4290973605 hasConcept C88478588 @default.
• W4290973605 hasConceptScore W4290973605C102414288 @default.
• W4290973605 hasConceptScore W4290973605C104317684 @default.
• W4290973605 hasConceptScore W4290973605C105580179 @default.
• W4290973605 hasConceptScore W4290973605C141231307 @default.
• W4290973605 hasConceptScore W4290973605C144024400 @default.
• W4290973605 hasConceptScore W4290973605C149364088 @default.
• W4290973605 hasConceptScore W4290973605C149923435 @default.
• W4290973605 hasConceptScore W4290973605C156695909 @default.
• W4290973605 hasConceptScore W4290973605C191908910 @default.
• W4290973605 hasConceptScore W4290973605C2777019660 @default.
• W4290973605 hasConceptScore W4290973605C2778918659 @default.
• W4290973605 hasConceptScore W4290973605C2779637612 @default.
• W4290973605 hasConceptScore W4290973605C2908647359 @default.

• next