Matches in SemOpenAlex for { <https://semopenalex.org/work/W4291008055> ?p ?o ?g. }
- W4291008055 endingPage "e0010649" @default.
- W4291008055 startingPage "e0010649" @default.
- W4291008055 abstract "Objective We conducted an abattoir-based cross-sectional study in the five administrative regions of Northern Ghana to determine the distribution of bovine tuberculosis (BTB) among slaughtered carcasses and identify the possibility of zoonotic transmission. Methods Direct smear microscopy was done on 438 tuberculosis-like lesions from selected cattle organs and cultured on Lowenstein-Jensen media. Acid-fast bacilli (AFB) isolates were confirmed as members of the Mycobacterium tuberculosis complex (MTBC) by PCR amplification of IS 6110 and rpo ß . Characterization and assignment into MTBC lineage and sub-lineage were done by spoligotyping, with the aid of the SITVIT2, miruvntrplus and mbovis.org databases. Spoligotype data was compared to that of clinical M . bovis isolates from the same regions to identify similarities. Results A total of 319/438 (72.8%) lesion homogenates were smear positive out of which, 84.6% (270/319) had microscopic grade of at least 1+ for AFB. Two hundred and sixty-five samples (265/438; 60.5%) were culture positive, of which 212 (80.0%) were MTBC. Approximately 16.7% (34/203) of the isolates with correctly defined spoligotypes were negative for IS 6110 PCR but were confirmed by rpo ß . Spoligotyping characterized 203 isolates as M . bovis (198, 97.5%), M . caprae (3, 1.5%), M . tuberculosis (Mtbss) lineage (L) 4 Cameroon sub-lineage, (1, 0.5%), and M . africanum (Maf) L6 (1, 0.5%). A total of 53 unique spoligotype patterns were identified across the five administrative regions (33 and 28 were identified as orphan respectively by the SITVIT2 and mbovis.org databases), with the most dominant spoligotype being SIT1037/ SB0944 (77/203, 37.93%). Analysis of the bovine and human M . bovis isolates showed 75% (3/4) human M . bovis isolates sharing the same spoligotype pattern with the bovine isolates. Conclusion Our study identified that approximately 29% of M . bovis strains causing BTB in Northern Ghana are caused by uncharacterized spoligotypes. Our findings suggest possible zoonotic transmission and highlight the need for BTB disease control in Northern Ghana." @default.
- W4291008055 created "2022-08-13" @default.
- W4291008055 creator A5002413808 @default.
- W4291008055 creator A5002775483 @default.
- W4291008055 creator A5003472363 @default.
- W4291008055 creator A5003899707 @default.
- W4291008055 creator A5006469888 @default.
- W4291008055 creator A5007673574 @default.
- W4291008055 creator A5013224926 @default.
- W4291008055 creator A5019763798 @default.
- W4291008055 creator A5033111278 @default.
- W4291008055 creator A5036103659 @default.
- W4291008055 creator A5039319685 @default.
- W4291008055 creator A5054200621 @default.
- W4291008055 creator A5059740116 @default.
- W4291008055 creator A5067413748 @default.
- W4291008055 creator A5086228742 @default.
- W4291008055 creator A5086400893 @default.
- W4291008055 date "2022-08-11" @default.
- W4291008055 modified "2023-09-30" @default.
- W4291008055 title "Molecular epidemiology of bovine tuberculosis in Northern Ghana identifies several uncharacterized bovine spoligotypes and suggests possible zoonotic transmission" @default.
- W4291008055 cites W1591474685 @default.
- W4291008055 cites W1825673675 @default.
- W4291008055 cites W1965727953 @default.
- W4291008055 cites W1966229360 @default.
- W4291008055 cites W1967422565 @default.
- W4291008055 cites W1969294471 @default.
- W4291008055 cites W1974721513 @default.
- W4291008055 cites W1985986410 @default.
- W4291008055 cites W1994498015 @default.
- W4291008055 cites W1999809115 @default.
- W4291008055 cites W2004610971 @default.
- W4291008055 cites W2011405738 @default.
- W4291008055 cites W2028112088 @default.
- W4291008055 cites W2028275031 @default.
- W4291008055 cites W2028762534 @default.
- W4291008055 cites W2043300230 @default.
- W4291008055 cites W2047978381 @default.
- W4291008055 cites W2054522685 @default.
- W4291008055 cites W2056965647 @default.
- W4291008055 cites W2072377247 @default.
- W4291008055 cites W2083889567 @default.
- W4291008055 cites W2086305793 @default.
- W4291008055 cites W2088628022 @default.
- W4291008055 cites W2093517437 @default.
- W4291008055 cites W2096162460 @default.
- W4291008055 cites W2098911548 @default.
- W4291008055 cites W2100246107 @default.
- W4291008055 cites W2106523569 @default.
- W4291008055 cites W2113265821 @default.
- W4291008055 cites W2113833420 @default.
- W4291008055 cites W2133613819 @default.
- W4291008055 cites W2134558351 @default.
- W4291008055 cites W2143950161 @default.
- W4291008055 cites W2147353041 @default.
- W4291008055 cites W2163052923 @default.
- W4291008055 cites W2164541993 @default.
- W4291008055 cites W2165029111 @default.
- W4291008055 cites W2165822919 @default.
- W4291008055 cites W2221018489 @default.
- W4291008055 cites W2294460080 @default.
- W4291008055 cites W2296481589 @default.
- W4291008055 cites W2338652248 @default.
- W4291008055 cites W2505967986 @default.
- W4291008055 cites W2514495836 @default.
- W4291008055 cites W2515979788 @default.
- W4291008055 cites W2570090939 @default.
- W4291008055 cites W2592956810 @default.
- W4291008055 cites W2603888074 @default.
- W4291008055 cites W2728004513 @default.
- W4291008055 cites W2760836682 @default.
- W4291008055 cites W2777245129 @default.
- W4291008055 cites W2787060742 @default.
- W4291008055 cites W2906585453 @default.
- W4291008055 cites W2915460228 @default.
- W4291008055 cites W2950624800 @default.
- W4291008055 cites W2953850242 @default.
- W4291008055 cites W2958927796 @default.
- W4291008055 cites W3021169526 @default.
- W4291008055 cites W3048650890 @default.
- W4291008055 cites W3107092404 @default.
- W4291008055 cites W3178324822 @default.
- W4291008055 cites W4234136247 @default.
- W4291008055 doi "https://doi.org/10.1371/journal.pntd.0010649" @default.
- W4291008055 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35951638" @default.
- W4291008055 hasPublicationYear "2022" @default.
- W4291008055 type Work @default.
- W4291008055 citedByCount "0" @default.
- W4291008055 crossrefType "journal-article" @default.
- W4291008055 hasAuthorship W4291008055A5002413808 @default.
- W4291008055 hasAuthorship W4291008055A5002775483 @default.
- W4291008055 hasAuthorship W4291008055A5003472363 @default.
- W4291008055 hasAuthorship W4291008055A5003899707 @default.
- W4291008055 hasAuthorship W4291008055A5006469888 @default.
- W4291008055 hasAuthorship W4291008055A5007673574 @default.
- W4291008055 hasAuthorship W4291008055A5013224926 @default.
- W4291008055 hasAuthorship W4291008055A5019763798 @default.
- W4291008055 hasAuthorship W4291008055A5033111278 @default.