Matches in SemOpenAlex for { <https://semopenalex.org/work/W4291036232> ?p ?o ?g. }
- W4291036232 endingPage "9" @default.
- W4291036232 startingPage "1" @default.
- W4291036232 abstract "Aim. To investigate the mechanism of progesterone inhibiting the scorch death of SH-SY5Y cells induced by exogenous adenosine triphosphate (ATP). Methods. SH-SY5Y cells with good logarithmic growth were used in the experiment. The cells were randomly divided into 5 groups: normal control group, DMSO group, BBG group, ATP group, and ATP+progesterone group. The cell survival rate of each group was measured by CCK-8 method. The expressions of P2X7 receptor, caspase-1, caspase-11, and IL-1β were detected by western blotting. Results. (1) After SH-SY5Y cells were treated with ATP at different concentrations (1, 3, 6, and 9 mmol/L) for 2 hours, the cell survival rate decreased in a concentration-dependent manner compared with the normal blank group. The results showed that the optimal lethal concentration of ATP was 6 mmol/L. SH-SY5Y cells were preincubated with progesterone at different concentrations (3, 10, 30, and 100 nmol/L) for 30 minutes and then incubated with 6 mmol/L ATP. The cell survival rate of this group was significantly improved ( <math xmlns=http://www.w3.org/1998/Math/MathML id=M1> <mi>P</mi> <mo><</mo> <mn>0.01</mn> </math> ). The optimal concentration of progesterone to improve cell survival and inhibit cell death was 30 nmol/L. (2) Compared to the control group, there was no significant difference ( <math xmlns=http://www.w3.org/1998/Math/MathML id=M2> <mi>P</mi> <mo>></mo> <mn>0.05</mn> </math> ) in P2X7 receptor, caspase-1, caspase-11, and IL-1β with the DMSO group (0.001% DMSO, 24 h) and BBG group (bbg1 mmol/L, 24 h). (3) In the ATP group, the expression of P2X7 receptor and caspase-1 (the key protein of classical cell death pathway) increased significantly ( <math xmlns=http://www.w3.org/1998/Math/MathML id=M3> <mi>P</mi> <mo><</mo> <mn>0.01</mn> </math> ), which was related to inflammatory factor IL-1β with consistent performance ( <math xmlns=http://www.w3.org/1998/Math/MathML id=M4> <mi>P</mi> <mo><</mo> <mn>0.01</mn> </math> ). There was no significant change in caspase-11 (the key protein of nonclassical focal death pathway) ( <math xmlns=http://www.w3.org/1998/Math/MathML id=M5> <mi>P</mi> <mo>></mo> <mn>0.05</mn> </math> ). (4) The expression of P2X7 receptor, caspase-1, and inflammatory factor IL-1β in the progesterone+ATP group was significantly downregulated ( <math xmlns=http://www.w3.org/1998/Math/MathML id=M6> <mi>P</mi> <mo><</mo> <mn>0.01</mn> </math> ). There was no significant change in caspase-11 ( <math xmlns=http://www.w3.org/1998/Math/MathML id=M7> <mi>P</mi> <mo>></mo> <mn>0.05</mn> </math> ). Conclusion. Certain dose of progesterone can inhibit the focal death of SH-SY5Y cells induced by extracellular high concentration ATP. It can reduce the expression of P2X7 receptor, inhibit the conduction pathway of cell death, reduce the release of inflammatory factor IL-1β, and improve cell survival." @default.
- W4291036232 created "2022-08-13" @default.
- W4291036232 creator A5005728980 @default.
- W4291036232 creator A5016679117 @default.
- W4291036232 creator A5018881082 @default.
- W4291036232 creator A5025303295 @default.
- W4291036232 creator A5043783755 @default.
- W4291036232 creator A5057026206 @default.
- W4291036232 creator A5074628946 @default.
- W4291036232 creator A5085083529 @default.
- W4291036232 date "2022-08-12" @default.
- W4291036232 modified "2023-10-14" @default.
- W4291036232 title "Progesterone Reduces ATP-Induced Pyroptosis of SH-SY5Y Cells" @default.
- W4291036232 cites W1501476081 @default.
- W4291036232 cites W1547831720 @default.
- W4291036232 cites W1832266951 @default.
- W4291036232 cites W1879915092 @default.
- W4291036232 cites W1932861877 @default.
- W4291036232 cites W1966060400 @default.
- W4291036232 cites W1979412100 @default.
- W4291036232 cites W1984520240 @default.
- W4291036232 cites W2002947389 @default.
- W4291036232 cites W2005462385 @default.
- W4291036232 cites W2016467209 @default.
- W4291036232 cites W2019407752 @default.
- W4291036232 cites W2041907993 @default.
- W4291036232 cites W2042811693 @default.
- W4291036232 cites W2050750460 @default.
- W4291036232 cites W2068517470 @default.
- W4291036232 cites W2080827095 @default.
- W4291036232 cites W2083536259 @default.
- W4291036232 cites W2084777655 @default.
- W4291036232 cites W2085895819 @default.
- W4291036232 cites W2120387391 @default.
- W4291036232 cites W2128354701 @default.
- W4291036232 cites W2132136505 @default.
- W4291036232 cites W2151191935 @default.
- W4291036232 cites W2155373881 @default.
- W4291036232 cites W2165904148 @default.
- W4291036232 cites W2167825597 @default.
- W4291036232 cites W2303481434 @default.
- W4291036232 cites W2418806051 @default.
- W4291036232 cites W2788640168 @default.
- W4291036232 cites W2801598991 @default.
- W4291036232 cites W2926689849 @default.
- W4291036232 cites W3045975352 @default.
- W4291036232 cites W3097432941 @default.
- W4291036232 cites W3116160502 @default.
- W4291036232 cites W3119025267 @default.
- W4291036232 cites W567799983 @default.
- W4291036232 doi "https://doi.org/10.1155/2022/4827444" @default.
- W4291036232 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35993057" @default.
- W4291036232 hasPublicationYear "2022" @default.
- W4291036232 type Work @default.
- W4291036232 citedByCount "2" @default.
- W4291036232 countsByYear W42910362322023 @default.
- W4291036232 crossrefType "journal-article" @default.
- W4291036232 hasAuthorship W4291036232A5005728980 @default.
- W4291036232 hasAuthorship W4291036232A5016679117 @default.
- W4291036232 hasAuthorship W4291036232A5018881082 @default.
- W4291036232 hasAuthorship W4291036232A5025303295 @default.
- W4291036232 hasAuthorship W4291036232A5043783755 @default.
- W4291036232 hasAuthorship W4291036232A5057026206 @default.
- W4291036232 hasAuthorship W4291036232A5074628946 @default.
- W4291036232 hasAuthorship W4291036232A5085083529 @default.
- W4291036232 hasBestOaLocation W42910362321 @default.
- W4291036232 hasConcept C126322002 @default.
- W4291036232 hasConcept C134018914 @default.
- W4291036232 hasConcept C153911025 @default.
- W4291036232 hasConcept C16685009 @default.
- W4291036232 hasConcept C170493617 @default.
- W4291036232 hasConcept C17172800 @default.
- W4291036232 hasConcept C185592680 @default.
- W4291036232 hasConcept C190283241 @default.
- W4291036232 hasConcept C2776715637 @default.
- W4291036232 hasConcept C2779083432 @default.
- W4291036232 hasConcept C2779564974 @default.
- W4291036232 hasConcept C31573885 @default.
- W4291036232 hasConcept C54355233 @default.
- W4291036232 hasConcept C55493867 @default.
- W4291036232 hasConcept C71924100 @default.
- W4291036232 hasConcept C81885089 @default.
- W4291036232 hasConcept C86803240 @default.
- W4291036232 hasConceptScore W4291036232C126322002 @default.
- W4291036232 hasConceptScore W4291036232C134018914 @default.
- W4291036232 hasConceptScore W4291036232C153911025 @default.
- W4291036232 hasConceptScore W4291036232C16685009 @default.
- W4291036232 hasConceptScore W4291036232C170493617 @default.
- W4291036232 hasConceptScore W4291036232C17172800 @default.
- W4291036232 hasConceptScore W4291036232C185592680 @default.
- W4291036232 hasConceptScore W4291036232C190283241 @default.
- W4291036232 hasConceptScore W4291036232C2776715637 @default.
- W4291036232 hasConceptScore W4291036232C2779083432 @default.
- W4291036232 hasConceptScore W4291036232C2779564974 @default.
- W4291036232 hasConceptScore W4291036232C31573885 @default.
- W4291036232 hasConceptScore W4291036232C54355233 @default.
- W4291036232 hasConceptScore W4291036232C55493867 @default.
- W4291036232 hasConceptScore W4291036232C71924100 @default.