FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4291097453> ?p ?o ?g. }

• W4291097453 abstract "Abstract Phosphoinositide 3-kinase (PI3K) p110δ signalling negatively regulates the production of mouse IgE. However, there are disparities between the mouse and human IgE biology, and the role of PI3K p110δ in the production of human IgE is yet to be determined. To investigate the effect of PI3K p110δ inhibition in the production of human IgE we isolated human B cells from tonsil tissue and stimulated them with IL-4 and anti-CD40 antibody to induce class switching to IgE and IgG1 in the presence or absence of IC87114, a small molecule inhibitor of PI3K p110δ. Using FACS, RT-PCR and ELISA we examined the effect of PI3K p110δ inhibition on IgE production and also determined the mechanisms involved. Unlike in mice, we observed that PI3K p110δ inhibition significantly reduces the number of IgE+ switched cells and the amounts of secreted IgE in IL4 and anti-CD40 cultures. However, the number of IgG1+ cells and secreted IgG1 were largely unaffected by PI3K p110δ inhibition. The expression levels of AID, ε and γ1 germinal transcripts or other factors involved in the regulation of CSR to IgE and IgG1 were also unaffected by IC87114. However, we found that IC87114 significantly decreases the proliferation of tonsil B cells stimulated with IL-4 and anti-CD40, specifically reducing the frequency of cells that had undergone 4 divisions or more. Our data identifies proliferation as the key mechanism through which PI3K p110δ regulates CSR of human B cells to IgE and highlights PI3K p110δ as a pharmacological target for the treatment of allergic disease." @default.
• W4291097453 created "2022-08-13" @default.
• W4291097453 creator A5005292473 @default.
• W4291097453 creator A5036777465 @default.
• W4291097453 creator A5052503822 @default.
• W4291097453 creator A5082690116 @default.
• W4291097453 creator A5087920627 @default.
• W4291097453 date "2022-08-09" @default.
• W4291097453 modified "2023-10-14" @default.
• W4291097453 title "Inhibition of PI3K p110δ activity reduces IgE production in IL-4 and anti-CD40 stimulated human B cell cultures" @default.
• W4291097453 cites W1686386964 @default.
• W4291097453 cites W1867136150 @default.
• W4291097453 cites W1964841658 @default.
• W4291097453 cites W1973422045 @default.
• W4291097453 cites W1977535836 @default.
• W4291097453 cites W1986835641 @default.
• W4291097453 cites W1994268130 @default.
• W4291097453 cites W2001713238 @default.
• W4291097453 cites W2005435096 @default.
• W4291097453 cites W2009342716 @default.
• W4291097453 cites W2012228539 @default.
• W4291097453 cites W2039533506 @default.
• W4291097453 cites W2040640809 @default.
• W4291097453 cites W2042450529 @default.
• W4291097453 cites W2059718955 @default.
• W4291097453 cites W2070361307 @default.
• W4291097453 cites W2070942879 @default.
• W4291097453 cites W2093262780 @default.
• W4291097453 cites W2093849821 @default.
• W4291097453 cites W2099044445 @default.
• W4291097453 cites W2103454590 @default.
• W4291097453 cites W2103470187 @default.
• W4291097453 cites W2106874385 @default.
• W4291097453 cites W2115518909 @default.
• W4291097453 cites W2123666692 @default.
• W4291097453 cites W2158538980 @default.
• W4291097453 cites W2161064364 @default.
• W4291097453 cites W2163577434 @default.
• W4291097453 cites W2315359128 @default.
• W4291097453 cites W2481181469 @default.
• W4291097453 cites W2728260204 @default.
• W4291097453 cites W3022998931 @default.
• W4291097453 cites W3170960533 @default.
• W4291097453 cites W4200391532 @default.
• W4291097453 cites W81528658 @default.
• W4291097453 doi "https://doi.org/10.1101/2022.08.09.503316" @default.
• W4291097453 hasPublicationYear "2022" @default.
• W4291097453 type Work @default.
• W4291097453 citedByCount "0" @default.
• W4291097453 crossrefType "posted-content" @default.
• W4291097453 hasAuthorship W4291097453A5005292473 @default.
• W4291097453 hasAuthorship W4291097453A5036777465 @default.
• W4291097453 hasAuthorship W4291097453A5052503822 @default.
• W4291097453 hasAuthorship W4291097453A5082690116 @default.
• W4291097453 hasAuthorship W4291097453A5087920627 @default.
• W4291097453 hasBestOaLocation W42910974531 @default.
• W4291097453 hasConcept C137756551 @default.
• W4291097453 hasConcept C141105273 @default.
• W4291097453 hasConcept C153911025 @default.
• W4291097453 hasConcept C154317977 @default.
• W4291097453 hasConcept C159654299 @default.
• W4291097453 hasConcept C185592680 @default.
• W4291097453 hasConcept C191732599 @default.
• W4291097453 hasConcept C202751555 @default.
• W4291097453 hasConcept C203014093 @default.
• W4291097453 hasConcept C2778453870 @default.
• W4291097453 hasConcept C2778690821 @default.
• W4291097453 hasConcept C39347974 @default.
• W4291097453 hasConcept C55493867 @default.
• W4291097453 hasConcept C62478195 @default.
• W4291097453 hasConcept C86554907 @default.
• W4291097453 hasConcept C86803240 @default.
• W4291097453 hasConcept C95444343 @default.
• W4291097453 hasConcept C96926380 @default.
• W4291097453 hasConceptScore W4291097453C137756551 @default.
• W4291097453 hasConceptScore W4291097453C141105273 @default.
• W4291097453 hasConceptScore W4291097453C153911025 @default.
• W4291097453 hasConceptScore W4291097453C154317977 @default.
• W4291097453 hasConceptScore W4291097453C159654299 @default.
• W4291097453 hasConceptScore W4291097453C185592680 @default.
• W4291097453 hasConceptScore W4291097453C191732599 @default.
• W4291097453 hasConceptScore W4291097453C202751555 @default.
• W4291097453 hasConceptScore W4291097453C203014093 @default.
• W4291097453 hasConceptScore W4291097453C2778453870 @default.
• W4291097453 hasConceptScore W4291097453C2778690821 @default.
• W4291097453 hasConceptScore W4291097453C39347974 @default.
• W4291097453 hasConceptScore W4291097453C55493867 @default.
• W4291097453 hasConceptScore W4291097453C62478195 @default.
• W4291097453 hasConceptScore W4291097453C86554907 @default.
• W4291097453 hasConceptScore W4291097453C86803240 @default.
• W4291097453 hasConceptScore W4291097453C95444343 @default.
• W4291097453 hasConceptScore W4291097453C96926380 @default.
• W4291097453 hasLocation W42910974531 @default.
• W4291097453 hasOpenAccess W4291097453 @default.
• W4291097453 hasPrimaryLocation W42910974531 @default.
• W4291097453 hasRelatedWork W1733081509 @default.
• W4291097453 hasRelatedWork W1780836642 @default.
• W4291097453 hasRelatedWork W2016307320 @default.
• W4291097453 hasRelatedWork W2106874385 @default.
• W4291097453 hasRelatedWork W2619770533 @default.

• next