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• W4291281069 endingPage "121734" @default.
• W4291281069 startingPage "121734" @default.
• W4291281069 abstract "Free copper ions (Cu+ and Cu2+) have critical toxicity to cells, although copper is an essential element for human body. Hence, sensitive monitoring is crucial to avoid over intake of Cu+/Cu2+. We herein designed a ssDNA sequence (A31) for synthetizing A31-templated silver nanoclusters (AgNCs), and demonstrated that Cu+/Cu2+ can induce the aggregation of A31-templated AgNCs and thus greatly enhanced the fluorescence emission of A31-templated AgNCs. Based on Cu+/Cu2+-induced fluorescence enhancement effect of A31-templated AgNCs, a label-free and signal-on fluorescent sensing platform was developed for the specific and sensitive detection of Cu+/Cu2+ in biological samples and intracellular imaging of Cu+/Cu2+ in cells. The signal-on fluorescent sensing platform could be used to rapidly detect Cu+ and Cu2+ with a detection limit of 0.1 µM within 30 min., and to perform the intracellular imaging of Cu+ and Cu2+ in cells with good cell permeability and biocompatibility. By using the signal-on fluorescent sensing platform, we have successfully detected Cu+ and Cu2+ in cells fluids and human serum with a recovery of 90-104% and a RSD (n = 5) < 5%, and performed the imaging of Cu+/Cu2+ in Hela cells. The developed fluorescent sensing platform has obvious analytical and imaging advantages such as signal-on, simple operation, short analysis time, both Cu+ and Cu2+ detection, similar or higher sensitivity, good cell permeability and biocompatibility, which promising a reliable approach for the rapid and on-site detection or imaging of free copper ions in biological samples in clinical diagnosis." @default.
• W4291281069 created "2022-08-14" @default.
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• W4291281069 date "2022-12-15" @default.
• W4291281069 modified "2023-10-16" @default.
• W4291281069 title "Sensitive detection and intracellular imaging of free copper ions based on DNA-templated silver nanoclusters aggregation-inducing fluorescence enhancement effect." @default.
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• W4291281069 doi "https://doi.org/10.1016/j.saa.2022.121734" @default.
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