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• W4291281566 abstract "The biological methanation of H 2 and CO 2 is beneficial to industrial upgrading of anaerobic digestion , efficient utilization of surplus electricity and H 2 , energy storage and peak regulation, as well as CO 2 emission reduction. Applying well-developed continuous stirred tank reactor (CSTR) for methanation is economical and convenient. In this study, the biological methanation process was conducted in a CSTR for 151 days. H 2 and CO 2 were the substrates and no external additives were added. The maximum CH 4 yield was 355.8 mL/(L·d) at a CH 4 content of 94.8%, and after double loading shock and recovery, the highest CH 4 content of 99.5% was achieved at a CH 4 yield of 249.3 mL/(L·d). The reactor could run stably at a pH around 8.5, and CO 2 flow was adjusted for pH control. As the experiment proceeded, hydrogenotrophic methanogens were enriched, and the microbial community in different stages had significant succession. Acetate was accumulated after loading shock, causing proportion increase of Natronincola , a genus of homoacetogen. Pseudomonas , an acetate-consuming and electroactive bacterium, was dominant in later period. Methanosarcina and Methanoculleus were in the majority of archaea, accounting for 80%–90%. In stable phase, 72.8% of the input CO 2 flowed to CH 4 , 17.8% consumed in rising pH, and 8.9% increased VFAs and microbes . As a complex system with competition and synergy among different reactions, the scientific design and regulation of the system to achieve an efficient methanation process is an important direction for future research. • Biological methanation of H 2 /CO 2 lasted 151 days in a CSTR with no nutrients added. • Maximum CH 4 yield was 355.8 mL/(L·d) at a CH 4 content of 94.8%. • Maximum CH 4 content was 99.5% at a CH 4 yield of 249.3 mL/(L·d). • Reactor ran stably at a pH around 8.5, and CO 2 flow was adjusted for pH control. • Succession of microbial community was analyzed and carbon flow was calculated." @default.
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• W4291281566 date "2022-10-01" @default.
• W4291281566 modified "2023-10-14" @default.
• W4291281566 title "Biological methanation of H2 and CO2 in a continuous stirred tank reactor" @default.
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• W4291281566 doi "https://doi.org/10.1016/j.jclepro.2022.133518" @default.
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