FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4291465478> ?p ?o ?g. }

• W4291465478 endingPage "9034" @default.
• W4291465478 startingPage "9034" @default.
• W4291465478 abstract "Background: We recently showed that a combined solution containing alpha-ketoglutarate (aKG) and 5-hydroxymethyl-furfural (5-HMF) has a solid antitumoral effect on the Jurkat cell line due to the fact of its antioxidative, caspase-3 and apoptosis activities, but no negative effect on human fibroblasts was obtained. The question arises how the single compounds, aKG and 5-HMF, affect peroxynitrite (ONOO−) and nitration of tyrosine residues, Jurkat cell proliferation and caspase-activated apoptosis. Methods: The ONOO− luminol-induced chemiluminescence reaction was used to measure the ONOO− scavenging function of aKG or 5-HMF, and their protection against nitration of tyrosine residues on bovine serum albumin was estimated with the ELISA technique. The Jurkat cell line was cultivated in the absence or presence of aKG or 5-HMF solutions between 0 and 3.5 µM aKG or 0 and 4 µM 5-HMF. Jurkat cells were tested for cell proliferation, mitochondrial activity and caspase-activated apoptosis. Results: aKG showed a concentration-dependent reduction in ONOO−, resulting in a 90% elimination of ONOO− using 200 mM aKG. In addition, 20 and 200 mM 5-HMF were able to reduce ONOO− only by 20%, while lower concentrations of 5-HMF remained stable in the presence of ONOO−. Nitration of tyrosine residues was inhibited 4 fold more effectively with 5-HMF compared to aKG measuring the IC50%. Both substances, aKG and 5-HMF, were shown to cause a reduction in Jurkat cell growth that was dependent on the dose and incubation time. The aKG effectively reduced Jurkat cell growth down to 50% after 48 and 72 h of incubation using the highest concentration of 3.5 µM, and 1, 1.6, 2, 3 and 4 µM 5-HMF inhibited any cell growth within (i) 24 h; 1.6, 2, 3 and 4 µM 5-HMF within 48 h (ii); 2, 3 and 4 µM 5-HMF within 72 h (iii). Furthermore, 4 µM was able to eliminate the starting cell number of 20,000 cells after 48 and 72 h down to 11,233 cells. The mitochondrial activity measurements supported the data on aKG or 5-HMF regarding cell growth in Jurkat cells, in both a dose- and incubation-time-dependent manner: the highest concentration of 3.5 µM aKG reduced the mitochondrial activity over 24 h (67.7%), 48 h (57.9%) and 72 h (46.8%) of incubation with Jurkat cells compared to the control incubation without aKG (100%). 5-HMF was more effective compared to aKG; the mitochondrial activity in the presence of 4 µM 5-HMF decreased after 24 h down to 68.4%, after 48 h to 42.9% and after 72 h to 32.0%. Moreover, 1.7 and 3.4 µM aKG had no effect on caspase-3-activated apoptosis (0.58% and 0.56%) in the Jurkat cell line. However, 2 and 4 µM 5-HMF increased the caspase-3-activated apoptosis up to 22.1% and 42.5% compared to the control (2.9%). A combined solution of 1.7 µM aKG + 0.7 µM 5-HMF showed a higher caspase-3-activated apoptosis (15.7%) compared to 1.7 µM aKG or 2 µM 5-HMF alone. In addition, 3.5 µM µg/mL aKG + 1.7 µM 5-HMF induced caspase-activated apoptosis up to 55.6% compared to 4.5% or 35.6% caspase-3 activity using 3.5 µM aKG or 4 µM 5-HMF. Conclusion: Both substances showed high antioxidative potential in eliminating either peroxynitrite or nitration of tyrosine residues, which results in a better inhibition of cell growth and mitochondrial activity of 5-HMF compared to aKG. However, caspase-3-activated apoptosis measurements revealed that the combination of both substances synergistically is the most effective compared to single compounds." @default.
• W4291465478 created "2022-08-15" @default.
• W4291465478 creator A5003056838 @default.
• W4291465478 creator A5006436950 @default.
• W4291465478 creator A5006723427 @default.
• W4291465478 creator A5021325083 @default.
• W4291465478 creator A5033815566 @default.
• W4291465478 creator A5041475415 @default.
• W4291465478 creator A5085313497 @default.
• W4291465478 date "2022-08-12" @default.
• W4291465478 modified "2023-10-01" @default.
• W4291465478 title "Alpha-Ketoglutarate or 5-HMF: Single Compounds Effectively Eliminate Leukemia Cells via Caspase-3 Apoptosis and Antioxidative Pathways" @default.
• W4291465478 cites W1886833444 @default.
• W4291465478 cites W1988595726 @default.
• W4291465478 cites W2005703314 @default.
• W4291465478 cites W2012634726 @default.
• W4291465478 cites W2055657360 @default.
• W4291465478 cites W2062483277 @default.
• W4291465478 cites W2079528494 @default.
• W4291465478 cites W2092218670 @default.
• W4291465478 cites W2098427962 @default.
• W4291465478 cites W2122386753 @default.
• W4291465478 cites W2123250752 @default.
• W4291465478 cites W2135706825 @default.
• W4291465478 cites W2137257509 @default.
• W4291465478 cites W2164636322 @default.
• W4291465478 cites W2218172750 @default.
• W4291465478 cites W2315146917 @default.
• W4291465478 cites W2403810324 @default.
• W4291465478 cites W2409659996 @default.
• W4291465478 cites W2787052956 @default.
• W4291465478 cites W2789621417 @default.
• W4291465478 cites W2896418082 @default.
• W4291465478 cites W2942814044 @default.
• W4291465478 cites W3042887236 @default.
• W4291465478 cites W3093675860 @default.
• W4291465478 cites W3196681785 @default.
• W4291465478 cites W3199006471 @default.
• W4291465478 cites W3212759604 @default.
• W4291465478 doi "https://doi.org/10.3390/ijms23169034" @default.
• W4291465478 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36012295" @default.
• W4291465478 hasPublicationYear "2022" @default.
• W4291465478 type Work @default.
• W4291465478 citedByCount "0" @default.
• W4291465478 crossrefType "journal-article" @default.
• W4291465478 hasAuthorship W4291465478A5003056838 @default.
• W4291465478 hasAuthorship W4291465478A5006436950 @default.
• W4291465478 hasAuthorship W4291465478A5006723427 @default.
• W4291465478 hasAuthorship W4291465478A5021325083 @default.
• W4291465478 hasAuthorship W4291465478A5033815566 @default.
• W4291465478 hasAuthorship W4291465478A5041475415 @default.
• W4291465478 hasAuthorship W4291465478A5085313497 @default.
• W4291465478 hasBestOaLocation W42914654781 @default.
• W4291465478 hasConcept C108634530 @default.
• W4291465478 hasConcept C178790620 @default.
• W4291465478 hasConcept C179464577 @default.
• W4291465478 hasConcept C181199279 @default.
• W4291465478 hasConcept C185592680 @default.
• W4291465478 hasConcept C190283241 @default.
• W4291465478 hasConcept C203014093 @default.
• W4291465478 hasConcept C2775856698 @default.
• W4291465478 hasConcept C2776090121 @default.
• W4291465478 hasConcept C2776165026 @default.
• W4291465478 hasConcept C2778784160 @default.
• W4291465478 hasConcept C2780795997 @default.
• W4291465478 hasConcept C54355233 @default.
• W4291465478 hasConcept C55493867 @default.
• W4291465478 hasConcept C62112901 @default.
• W4291465478 hasConcept C81885089 @default.
• W4291465478 hasConcept C86803240 @default.
• W4291465478 hasConcept C8891405 @default.
• W4291465478 hasConceptScore W4291465478C108634530 @default.
• W4291465478 hasConceptScore W4291465478C178790620 @default.
• W4291465478 hasConceptScore W4291465478C179464577 @default.
• W4291465478 hasConceptScore W4291465478C181199279 @default.
• W4291465478 hasConceptScore W4291465478C185592680 @default.
• W4291465478 hasConceptScore W4291465478C190283241 @default.
• W4291465478 hasConceptScore W4291465478C203014093 @default.
• W4291465478 hasConceptScore W4291465478C2775856698 @default.
• W4291465478 hasConceptScore W4291465478C2776090121 @default.
• W4291465478 hasConceptScore W4291465478C2776165026 @default.
• W4291465478 hasConceptScore W4291465478C2778784160 @default.
• W4291465478 hasConceptScore W4291465478C2780795997 @default.
• W4291465478 hasConceptScore W4291465478C54355233 @default.
• W4291465478 hasConceptScore W4291465478C55493867 @default.
• W4291465478 hasConceptScore W4291465478C62112901 @default.
• W4291465478 hasConceptScore W4291465478C81885089 @default.
• W4291465478 hasConceptScore W4291465478C86803240 @default.
• W4291465478 hasConceptScore W4291465478C8891405 @default.
• W4291465478 hasIssue "16" @default.
• W4291465478 hasLocation W42914654781 @default.
• W4291465478 hasLocation W42914654782 @default.
• W4291465478 hasLocation W42914654783 @default.
• W4291465478 hasOpenAccess W4291465478 @default.
• W4291465478 hasPrimaryLocation W42914654781 @default.
• W4291465478 hasRelatedWork W1979415715 @default.
• W4291465478 hasRelatedWork W1986871839 @default.
• W4291465478 hasRelatedWork W1994437526 @default.

• next