Matches in SemOpenAlex for { <https://semopenalex.org/work/W4291805677> ?p ?o ?g. }
- W4291805677 endingPage "1893" @default.
- W4291805677 startingPage "1883" @default.
- W4291805677 abstract "Enzyme-catalyzed hydrolysis is a fundamental chemical transformation involved in many essential metabolic processes. The enzyme 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (MTAN) catalyzes the hydrolysis of adenosine-containing metabolites in cysteine and methionine metabolism. Although MTAN enzymes contain highly similar active site architecture and generally follow a dissociative (DN*AN) reaction mechanism, substantial differences in reaction rates and chemical transition state structures have been reported. To understand how subtle changes in sequence and structure give rise to differences in chemistry between homologous enzymes, we have probed the reaction coordinates of two MTAN enzymes using quantum mechanical/molecular mechanical and molecular dynamics simulations combined with experimental methods. We show that the transition state structure and energy are significantly affected by the recruitment and positioning of the catalytic water molecule and that subtle differences in the noncatalytic active site residues alter the environment of the catalytic water, leading to changes in the reaction coordinate and observed reaction rate." @default.
- W4291805677 created "2022-08-16" @default.
- W4291805677 creator A5001475452 @default.
- W4291805677 creator A5007014988 @default.
- W4291805677 creator A5026970859 @default.
- W4291805677 date "2022-08-15" @default.
- W4291805677 modified "2023-09-26" @default.
- W4291805677 title "Precise Positioning of Water Is Critical for Hydrolysis Catalyzed by 5′-Methylthioadenosine Nucleosidase" @default.
- W4291805677 cites W1973649172 @default.
- W4291805677 cites W1977188687 @default.
- W4291805677 cites W1980803164 @default.
- W4291805677 cites W1990305736 @default.
- W4291805677 cites W1990341002 @default.
- W4291805677 cites W1997772366 @default.
- W4291805677 cites W2005474992 @default.
- W4291805677 cites W2011721714 @default.
- W4291805677 cites W2029667189 @default.
- W4291805677 cites W2031168104 @default.
- W4291805677 cites W2037133346 @default.
- W4291805677 cites W2049682677 @default.
- W4291805677 cites W2051027461 @default.
- W4291805677 cites W2059299942 @default.
- W4291805677 cites W2060433756 @default.
- W4291805677 cites W2067444931 @default.
- W4291805677 cites W2068877577 @default.
- W4291805677 cites W2070753604 @default.
- W4291805677 cites W2076168781 @default.
- W4291805677 cites W2086141419 @default.
- W4291805677 cites W2092995073 @default.
- W4291805677 cites W2097147953 @default.
- W4291805677 cites W2105279142 @default.
- W4291805677 cites W2114684175 @default.
- W4291805677 cites W2118101954 @default.
- W4291805677 cites W2122441538 @default.
- W4291805677 cites W2152300317 @default.
- W4291805677 cites W2153856953 @default.
- W4291805677 cites W2154278700 @default.
- W4291805677 cites W2158250132 @default.
- W4291805677 cites W2170803020 @default.
- W4291805677 cites W2331504047 @default.
- W4291805677 cites W2532366952 @default.
- W4291805677 cites W2550995848 @default.
- W4291805677 cites W2751446368 @default.
- W4291805677 cites W2766142559 @default.
- W4291805677 cites W2890944115 @default.
- W4291805677 cites W2891358505 @default.
- W4291805677 cites W2892007455 @default.
- W4291805677 cites W3005050488 @default.
- W4291805677 cites W3046751266 @default.
- W4291805677 cites W3112376646 @default.
- W4291805677 cites W3158684549 @default.
- W4291805677 cites W4242040668 @default.
- W4291805677 doi "https://doi.org/10.1021/acs.biochem.2c00351" @default.
- W4291805677 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35969806" @default.
- W4291805677 hasPublicationYear "2022" @default.
- W4291805677 type Work @default.
- W4291805677 citedByCount "0" @default.
- W4291805677 crossrefType "journal-article" @default.
- W4291805677 hasAuthorship W4291805677A5001475452 @default.
- W4291805677 hasAuthorship W4291805677A5007014988 @default.
- W4291805677 hasAuthorship W4291805677A5026970859 @default.
- W4291805677 hasConcept C147597530 @default.
- W4291805677 hasConcept C161790260 @default.
- W4291805677 hasConcept C178790620 @default.
- W4291805677 hasConcept C181199279 @default.
- W4291805677 hasConcept C18553476 @default.
- W4291805677 hasConcept C185592680 @default.
- W4291805677 hasConcept C2779201268 @default.
- W4291805677 hasConcept C32909587 @default.
- W4291805677 hasConcept C41183919 @default.
- W4291805677 hasConcept C55493867 @default.
- W4291805677 hasConcept C69118441 @default.
- W4291805677 hasConcept C71240020 @default.
- W4291805677 hasConcept C89031862 @default.
- W4291805677 hasConcept C94412978 @default.
- W4291805677 hasConceptScore W4291805677C147597530 @default.
- W4291805677 hasConceptScore W4291805677C161790260 @default.
- W4291805677 hasConceptScore W4291805677C178790620 @default.
- W4291805677 hasConceptScore W4291805677C181199279 @default.
- W4291805677 hasConceptScore W4291805677C18553476 @default.
- W4291805677 hasConceptScore W4291805677C185592680 @default.
- W4291805677 hasConceptScore W4291805677C2779201268 @default.
- W4291805677 hasConceptScore W4291805677C32909587 @default.
- W4291805677 hasConceptScore W4291805677C41183919 @default.
- W4291805677 hasConceptScore W4291805677C55493867 @default.
- W4291805677 hasConceptScore W4291805677C69118441 @default.
- W4291805677 hasConceptScore W4291805677C71240020 @default.
- W4291805677 hasConceptScore W4291805677C89031862 @default.
- W4291805677 hasConceptScore W4291805677C94412978 @default.
- W4291805677 hasFunder F4320334749 @default.
- W4291805677 hasIssue "17" @default.
- W4291805677 hasLocation W42918056771 @default.
- W4291805677 hasLocation W42918056772 @default.
- W4291805677 hasOpenAccess W4291805677 @default.
- W4291805677 hasPrimaryLocation W42918056771 @default.
- W4291805677 hasRelatedWork W1605921437 @default.
- W4291805677 hasRelatedWork W1969765414 @default.
- W4291805677 hasRelatedWork W1977794494 @default.