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- W4292103643 abstract "Abstract Key message Our results show that SPL12 plays a crucial role in regulating nodule development in Medicago sativa L. (alfalfa), and that AGL6 is targeted and downregulated by SPL12. Root architecture in plants is critical because of its role in controlling nutrient cycling, water use efficiency and response to biotic and abiotic stress factors. The small RNA, microRNA156 (miR156), is highly conserved in plants, where it functions by silencing a group of SQUAMOSA-PROMOTER BINDING PROTEIN-LIKE (SPL) transcription factors. We previously showed that transgenic Medicago sativa (alfalfa) plants overexpressing miR156 display increased nodulation, improved nitrogen fixation and enhanced root regenerative capacity during vegetative propagation. In alfalfa, transcripts of eleven SPL s, including SPL12 , are targeted for cleavage by miR156 . In this study, we characterized the role of SPL12 in root architecture and nodulation by investigating the transcriptomic and phenotypic changes associated with altered transcript levels of SPL12 , and by determining SPL12 regulatory targets using SPL12- silencing and –overexpressing alfalfa plants. Phenotypic analyses showed that silencing of SPL12 in alfalfa caused an increase in root regeneration, nodulation, and nitrogen fixation. In addition, AGL6 which encodes AGAMOUS-like MADS box transcription factor, was identified as being directly targeted for silencing by SPL12, based on Next Generation Sequencing-mediated transcriptome analysis and chromatin immunoprecipitation assays. Taken together, our results suggest that SPL12 and AGL6 form a genetic module that regulates root development and nodulation in alfalfa." @default.
- W4292103643 created "2022-08-17" @default.
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- W4292103643 date "2022-08-17" @default.
- W4292103643 modified "2023-10-01" @default.
- W4292103643 title "Deciphering the role of SPL12 and AGL6 from a genetic module that functions in nodulation and root regeneration in Medicago sativa" @default.
- W4292103643 cites W1018653398 @default.
- W4292103643 cites W1488757479 @default.
- W4292103643 cites W1524365075 @default.
- W4292103643 cites W1537401534 @default.
- W4292103643 cites W1563698537 @default.
- W4292103643 cites W1626869794 @default.
- W4292103643 cites W1963665019 @default.
- W4292103643 cites W1969353942 @default.
- W4292103643 cites W1972647460 @default.
- W4292103643 cites W1975109889 @default.
- W4292103643 cites W1981147036 @default.
- W4292103643 cites W1983743684 @default.
- W4292103643 cites W1985053764 @default.
- W4292103643 cites W1985403125 @default.
- W4292103643 cites W1987258650 @default.
- W4292103643 cites W1988694572 @default.
- W4292103643 cites W1991941346 @default.
- W4292103643 cites W1993780028 @default.
- W4292103643 cites W1999845170 @default.
- W4292103643 cites W2000331546 @default.
- W4292103643 cites W2000480520 @default.
- W4292103643 cites W2003696779 @default.
- W4292103643 cites W2006155982 @default.
- W4292103643 cites W2008391336 @default.
- W4292103643 cites W2010248837 @default.
- W4292103643 cites W2019808954 @default.
- W4292103643 cites W2022360143 @default.
- W4292103643 cites W2028773549 @default.
- W4292103643 cites W2029565291 @default.
- W4292103643 cites W2050460033 @default.
- W4292103643 cites W2052374326 @default.
- W4292103643 cites W2055746239 @default.
- W4292103643 cites W2058014822 @default.
- W4292103643 cites W2058229105 @default.
- W4292103643 cites W2068415331 @default.
- W4292103643 cites W2075717543 @default.
- W4292103643 cites W2077736423 @default.
- W4292103643 cites W2085984763 @default.
- W4292103643 cites W2090663374 @default.
- W4292103643 cites W2096718408 @default.
- W4292103643 cites W2099058702 @default.
- W4292103643 cites W2100305481 @default.
- W4292103643 cites W2100638755 @default.
- W4292103643 cites W2101749620 @default.
- W4292103643 cites W2102897805 @default.
- W4292103643 cites W2102942796 @default.
- W4292103643 cites W2105344969 @default.
- W4292103643 cites W2106250466 @default.
- W4292103643 cites W2107725916 @default.
- W4292103643 cites W2109895782 @default.
- W4292103643 cites W2113355569 @default.
- W4292103643 cites W2123313379 @default.
- W4292103643 cites W2123749465 @default.
- W4292103643 cites W2127920639 @default.
- W4292103643 cites W2130357866 @default.
- W4292103643 cites W2130527936 @default.
- W4292103643 cites W2139244050 @default.
- W4292103643 cites W2145510946 @default.
- W4292103643 cites W2147732685 @default.
- W4292103643 cites W2148497807 @default.
- W4292103643 cites W2150204365 @default.
- W4292103643 cites W2151196388 @default.
- W4292103643 cites W2155012764 @default.
- W4292103643 cites W2156059537 @default.
- W4292103643 cites W2157255799 @default.
- W4292103643 cites W2158861015 @default.
- W4292103643 cites W2158918882 @default.
- W4292103643 cites W2159106053 @default.
- W4292103643 cites W2160737163 @default.
- W4292103643 cites W2162871766 @default.
- W4292103643 cites W2165557989 @default.
- W4292103643 cites W2168178601 @default.
- W4292103643 cites W2169597291 @default.
- W4292103643 cites W2172789080 @default.
- W4292103643 cites W2206180507 @default.
- W4292103643 cites W2220750614 @default.
- W4292103643 cites W2403783237 @default.
- W4292103643 cites W2406141992 @default.
- W4292103643 cites W2486964564 @default.
- W4292103643 cites W2507161241 @default.
- W4292103643 cites W2508987044 @default.
- W4292103643 cites W2517167461 @default.
- W4292103643 cites W2583412919 @default.
- W4292103643 cites W2619023234 @default.
- W4292103643 cites W2728882170 @default.
- W4292103643 cites W2755900479 @default.
- W4292103643 cites W2769807097 @default.