FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4292169150> ?p ?o ?g. }

• W4292169150 abstract "Glucoraphanin (GRA) is present in the seeds and nutrient organs of broccoli and is the precursor of the anti-cancer compound sulforaphane (SF). The hairy roots obtained by infecting broccoli (Brassica oleracea L. var. Italic Planch) leaves with Agrobacterium rhizogenes (ATCC15834) are phytohormonally autonomous, genetically stable, and can produce large amounts of the anti-cancer substance SF. Melatonin (MT) is a natural hormone widely found in plants. Studies have shown that melatonin can regulate the synthesis of secondary metabolites of downstream targets by mediating the synthesis of signal molecules. However, whether MT regulates the synthesis of NO and H2O2 and mediates the synthesis mechanism of secondary metabolites, GRA and SF, is not yet clear. In this study, the hairy roots of broccoli were treated with 500 μmol/L MT, and the genome of broccoli (Brassica oleracea L. var. botrytis L) was used as the reference genome for transcriptome analysis. By this approach, we found that MT regulates the synthesis of NO and H2O2 and mediates the synthesis of secondary metabolites GRA and SF. GO annotations indicated that DEGs involved in the MT treatment of broccoli hairy roots were mainly related to catalytic activity, cells, and metabolic processes; the KEGG pathway analysis indicated that MT treatment likely affects the hormone signal transduction process in broccoli hairy roots; broccoli hairy roots were treated with 500 μmol/L MT for 0, 6, 12, 20, and 32 h, respectively; compared with 0 h, the yield of GRA and SF increased under the other treatments. The highest yields of GRA and SF occurred at 12 h. The NO content was the highest at 12 h, and the H2O2 content was positively correlated with MT concentration. The content of NO and H2O2 were regulated, and the content of GRA and SF was increased under MT treatment. NO synthase inhibitor (L-NAME and TUN) could effectively inhibit the content of NO in broccoli hairy roots and reduce GRA and SF yield; MT could regulate NO levels by regulating NO synthesis-related enzymes and could alleviate the reduction of NO content in tissue cells caused by NO synthase inhibitor and promote NO synthesis. These results have important theoretical implications for understanding the regulation of GRA and SF synthesis events by NO and H2O2." @default.
• W4292169150 created "2022-08-18" @default.
• W4292169150 creator A5002782106 @default.
• W4292169150 creator A5004421185 @default.
• W4292169150 creator A5012157159 @default.
• W4292169150 creator A5016570537 @default.
• W4292169150 creator A5026512168 @default.
• W4292169150 creator A5030130088 @default.
• W4292169150 creator A5042956973 @default.
• W4292169150 creator A5046839632 @default.
• W4292169150 creator A5067418483 @default.
• W4292169150 creator A5082548972 @default.
• W4292169150 date "2022-08-17" @default.
• W4292169150 modified "2023-10-10" @default.
• W4292169150 title "Glucoraphanin and sulforaphane biosynthesis by melatonin mediating nitric oxide in hairy roots of broccoli (Brassica oleracea L. var. italica Planch): insights from transcriptome data" @default.
• W4292169150 cites W1966813038 @default.
• W4292169150 cites W1976168981 @default.
• W4292169150 cites W1995384886 @default.
• W4292169150 cites W1999522152 @default.
• W4292169150 cites W2032902113 @default.
• W4292169150 cites W2036445447 @default.
• W4292169150 cites W2042415379 @default.
• W4292169150 cites W2051326295 @default.
• W4292169150 cites W2060459639 @default.
• W4292169150 cites W2061974378 @default.
• W4292169150 cites W2094742052 @default.
• W4292169150 cites W2099299146 @default.
• W4292169150 cites W2105106534 @default.
• W4292169150 cites W2107060225 @default.
• W4292169150 cites W2107277218 @default.
• W4292169150 cites W2117276379 @default.
• W4292169150 cites W2118680350 @default.
• W4292169150 cites W2136683403 @default.
• W4292169150 cites W2141293774 @default.
• W4292169150 cites W2154929766 @default.
• W4292169150 cites W2159482845 @default.
• W4292169150 cites W2192080449 @default.
• W4292169150 cites W2328790886 @default.
• W4292169150 cites W2399284521 @default.
• W4292169150 cites W2523418205 @default.
• W4292169150 cites W2552188114 @default.
• W4292169150 cites W2568046757 @default.
• W4292169150 cites W2758782039 @default.
• W4292169150 cites W2761769539 @default.
• W4292169150 cites W2905459835 @default.
• W4292169150 cites W2912354183 @default.
• W4292169150 cites W2944045830 @default.
• W4292169150 cites W2969218120 @default.
• W4292169150 cites W2978282709 @default.
• W4292169150 cites W3014008743 @default.
• W4292169150 cites W3016259897 @default.
• W4292169150 cites W3031721722 @default.
• W4292169150 cites W3049505483 @default.
• W4292169150 cites W3109343414 @default.
• W4292169150 cites W3120782005 @default.
• W4292169150 cites W3123085391 @default.
• W4292169150 cites W3201427213 @default.
• W4292169150 cites W4294216483 @default.
• W4292169150 doi "https://doi.org/10.1186/s12870-022-03747-x" @default.
• W4292169150 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35974315" @default.
• W4292169150 hasPublicationYear "2022" @default.
• W4292169150 type Work @default.
• W4292169150 citedByCount "1" @default.
• W4292169150 crossrefType "journal-article" @default.
• W4292169150 hasAuthorship W4292169150A5002782106 @default.
• W4292169150 hasAuthorship W4292169150A5004421185 @default.
• W4292169150 hasAuthorship W4292169150A5012157159 @default.
• W4292169150 hasAuthorship W4292169150A5016570537 @default.
• W4292169150 hasAuthorship W4292169150A5026512168 @default.
• W4292169150 hasAuthorship W4292169150A5030130088 @default.
• W4292169150 hasAuthorship W4292169150A5042956973 @default.
• W4292169150 hasAuthorship W4292169150A5046839632 @default.
• W4292169150 hasAuthorship W4292169150A5067418483 @default.
• W4292169150 hasAuthorship W4292169150A5082548972 @default.
• W4292169150 hasBestOaLocation W42921691501 @default.
• W4292169150 hasConcept C104317684 @default.
• W4292169150 hasConcept C105409712 @default.
• W4292169150 hasConcept C150194340 @default.
• W4292169150 hasConcept C152724338 @default.
• W4292169150 hasConcept C162317418 @default.
• W4292169150 hasConcept C2775943906 @default.
• W4292169150 hasConcept C2776138632 @default.
• W4292169150 hasConcept C2776747608 @default.
• W4292169150 hasConcept C2777943035 @default.
• W4292169150 hasConcept C2778164613 @default.
• W4292169150 hasConcept C2780841255 @default.
• W4292169150 hasConcept C553450214 @default.
• W4292169150 hasConcept C55493867 @default.
• W4292169150 hasConcept C59822182 @default.
• W4292169150 hasConcept C86803240 @default.
• W4292169150 hasConceptScore W4292169150C104317684 @default.
• W4292169150 hasConceptScore W4292169150C105409712 @default.
• W4292169150 hasConceptScore W4292169150C150194340 @default.
• W4292169150 hasConceptScore W4292169150C152724338 @default.
• W4292169150 hasConceptScore W4292169150C162317418 @default.
• W4292169150 hasConceptScore W4292169150C2775943906 @default.
• W4292169150 hasConceptScore W4292169150C2776138632 @default.
• W4292169150 hasConceptScore W4292169150C2776747608 @default.
• W4292169150 hasConceptScore W4292169150C2777943035 @default.
• W4292169150 hasConceptScore W4292169150C2778164613 @default.

• next