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• W4292292972 endingPage "10074" @default.
• W4292292972 startingPage "10065" @default.
• W4292292972 abstract "Acrylamide is a neurotoxin and carcinogen that forms during the thermal processing of food, inflicting irreversible harm to human health. Herein, a ratiometric fluorescence biosensor based on a 6-carboxyfluorescein-labeled aptamer (FAM-ssDNA) and porphyrin metal–organic framework (PCN-224) was developed. PCN-224 exhibits strong adsorption capacity for FAM-ssDNA and also quenches the fluorescence of FAM-ssDNA via fluorescence resonance energy transfer and photoinduced electron transfer. FAM-ssDNA hybridizes with complementary DNA to form double-stranded DNA (FAM-dsDNA), which is liberated from the PCN-224 surface, resulting in fluorescence recovery. However, the intrinsic fluorescence of the ligand remains unchanged. Acrylamide can create an adduct with FAM-ssDNA and inhibit the hybridization of FAM-dsDNA, thus realizing ratiometric sensing of acrylamide. The proposed biosensor displays excellent detection performance from 10 nM∼0.5 mM with a limit of detection of 1.9 nM. In conclusion, a fabricated biosensor was successfully applied to detect acrylamide in thermally processed food, and the results were consistent with those of high-performance liquid chromatography." @default.
• W4292292972 created "2022-08-19" @default.
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• W4292292972 date "2022-08-08" @default.
• W4292292972 modified "2023-10-17" @default.
• W4292292972 title "Ratiometric Fluorescent Metal–Organic Framework Biosensor for Ultrasensitive Detection of Acrylamide" @default.
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• W4292292972 doi "https://doi.org/10.1021/acs.jafc.2c04756" @default.
• W4292292972 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35939824" @default.
• W4292292972 hasPublicationYear "2022" @default.
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