FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4292548630> ?p ?o ?g. }

• W4292548630 endingPage "4018" @default.
• W4292548630 startingPage "4018" @default.
• W4292548630 abstract "Background: TERT promoter methylation, located several hundred base pairs upstream of the transcriptional start site, is cancer specific and correlates with increased TERT mRNA expression and poorer patient outcome. Promoter methylation, however, is not mutually exclusive to TERT activating genetic alterations, as predicted for functionally redundant mechanisms. To annotate the altered patterns of TERT promoter methylation and their relationship with gene expression, we applied a Pacific Biosciences-based, long-read, bisulfite-sequencing technology and compared the differences in the methylation marks between wild-type and mutant cancers in an allele-specific manner. Results: We cataloged TERT genetic alterations (i.e., promoter point mutations or structural variations), allele-specific promoter methylation patterns, and allele-specific expression levels in a cohort of 54 cancer cell lines. In heterozygous mutant cell lines, the mutant alleles were significantly less methylated than their silent, mutation-free alleles (p < 0.05). In wild-type cell lines, by contrast, both epialleles were equally methylated to high levels at the TERT distal promoter, but differentially methylated in the proximal regions. ChIP analysis showed that epialleles with the hypomethylated proximal and core promoter were enriched in the active histone mark H3K4me2/3, whereas epialleles that were methylated in those regions were enriched in the repressive histone mark H3K27me3. Decitabine therapy induced biallelic expression in the wild-type cancer cells, whereas the mutant cell lines were unaffected. Conclusions: Long-read bisulfite sequencing analysis revealed differences in the methylation profiles and responses to demethylating agents between TERT wild-type and genetically altered cancer cell lines. The causal relation between TERT promoter methylation and gene expression remains to be established." @default.
• W4292548630 created "2022-08-22" @default.
• W4292548630 creator A5007239425 @default.
• W4292548630 creator A5010983473 @default.
• W4292548630 creator A5020183452 @default.
• W4292548630 creator A5020547706 @default.
• W4292548630 creator A5026730026 @default.
• W4292548630 creator A5030319840 @default.
• W4292548630 creator A5038649340 @default.
• W4292548630 creator A5044032877 @default.
• W4292548630 creator A5064415817 @default.
• W4292548630 creator A5069804787 @default.
• W4292548630 creator A5084373941 @default.
• W4292548630 creator A5089297467 @default.
• W4292548630 date "2022-08-19" @default.
• W4292548630 modified "2023-10-15" @default.
• W4292548630 title "Targeted Long-Read Bisulfite Sequencing Identifies Differences in the TERT Promoter Methylation Profiles between TERT Wild-Type and TERT Mutant Cancer Cells" @default.
• W4292548630 cites W1517842284 @default.
• W4292548630 cites W1560916735 @default.
• W4292548630 cites W1878249864 @default.
• W4292548630 cites W1950829641 @default.
• W4292548630 cites W1967064280 @default.
• W4292548630 cites W1977169326 @default.
• W4292548630 cites W1978329681 @default.
• W4292548630 cites W1980197198 @default.
• W4292548630 cites W1995630361 @default.
• W4292548630 cites W2002585385 @default.
• W4292548630 cites W2002625908 @default.
• W4292548630 cites W2007576179 @default.
• W4292548630 cites W2008077097 @default.
• W4292548630 cites W2030209169 @default.
• W4292548630 cites W2043640738 @default.
• W4292548630 cites W2055902044 @default.
• W4292548630 cites W2069698023 @default.
• W4292548630 cites W2070191145 @default.
• W4292548630 cites W2073495669 @default.
• W4292548630 cites W2098744299 @default.
• W4292548630 cites W2103251300 @default.
• W4292548630 cites W2105678404 @default.
• W4292548630 cites W2111274140 @default.
• W4292548630 cites W2116993454 @default.
• W4292548630 cites W2121744576 @default.
• W4292548630 cites W2123463167 @default.
• W4292548630 cites W2131117209 @default.
• W4292548630 cites W2131374955 @default.
• W4292548630 cites W2143925305 @default.
• W4292548630 cites W2148187752 @default.
• W4292548630 cites W2156250877 @default.
• W4292548630 cites W2156509269 @default.
• W4292548630 cites W2160562062 @default.
• W4292548630 cites W2166889662 @default.
• W4292548630 cites W2171256063 @default.
• W4292548630 cites W2221710132 @default.
• W4292548630 cites W2222675003 @default.
• W4292548630 cites W2235405793 @default.
• W4292548630 cites W2294769548 @default.
• W4292548630 cites W2296878526 @default.
• W4292548630 cites W2466351482 @default.
• W4292548630 cites W2508099675 @default.
• W4292548630 cites W2581179619 @default.
• W4292548630 cites W2583025286 @default.
• W4292548630 cites W2604551370 @default.
• W4292548630 cites W2608545378 @default.
• W4292548630 cites W2775050647 @default.
• W4292548630 cites W2776280640 @default.
• W4292548630 cites W2782084467 @default.
• W4292548630 cites W2793548086 @default.
• W4292548630 cites W2803325987 @default.
• W4292548630 cites W2890068857 @default.
• W4292548630 cites W2896689522 @default.
• W4292548630 cites W2898125316 @default.
• W4292548630 cites W2898215009 @default.
• W4292548630 cites W2902229746 @default.
• W4292548630 cites W2911343585 @default.
• W4292548630 cites W2944727022 @default.
• W4292548630 cites W2971151221 @default.
• W4292548630 cites W3029732203 @default.
• W4292548630 cites W3049102632 @default.
• W4292548630 doi "https://doi.org/10.3390/cancers14164018" @default.
• W4292548630 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36011010" @default.
• W4292548630 hasPublicationYear "2022" @default.
• W4292548630 type Work @default.
• W4292548630 citedByCount "2" @default.
• W4292548630 countsByYear W42925486302022 @default.
• W4292548630 countsByYear W42925486302023 @default.
• W4292548630 crossrefType "journal-article" @default.
• W4292548630 hasAuthorship W4292548630A5007239425 @default.
• W4292548630 hasAuthorship W4292548630A5010983473 @default.
• W4292548630 hasAuthorship W4292548630A5020183452 @default.
• W4292548630 hasAuthorship W4292548630A5020547706 @default.
• W4292548630 hasAuthorship W4292548630A5026730026 @default.
• W4292548630 hasAuthorship W4292548630A5030319840 @default.
• W4292548630 hasAuthorship W4292548630A5038649340 @default.
• W4292548630 hasAuthorship W4292548630A5044032877 @default.
• W4292548630 hasAuthorship W4292548630A5064415817 @default.
• W4292548630 hasAuthorship W4292548630A5069804787 @default.
• W4292548630 hasAuthorship W4292548630A5084373941 @default.
• W4292548630 hasAuthorship W4292548630A5089297467 @default.

• next