FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4292805493> ?p ?o ?g. }

• W4292805493 endingPage "102448" @default.
• W4292805493 startingPage "102448" @default.
• W4292805493 abstract "The inter-relationship between microglia dynamics and oxidative stress (Ox-stress) in dystrophic neurites (DNs) at Alzheimer’s Disease (AD) plaques may contribute to the pathological changes in neurons. We developed new in vivo imaging strategies to combine EGFP expression in microglia with neuronal expression of genetically encoded ratiometric redox sensors (rogRFP2 or roGFP1), and immunohistochemistry to investigate how microglia influence Ox-stress at amyloid plaques in 5xFAD AD mice. By simultaneously imaging microglia morphology and neuronal Ox-stress over time in vivo and in fixed brains we found that microglia preferentially enwrapped DNs exhibiting the greatest degree of Ox-stress. After microglia were partially depleted with the CSF1 receptor antagonist PLX3397, Ox-stress in DNs increased in a manner that was inversely correlated to the extent of coverage of the adjacent Aβ plaques by the remaining microglia. These data suggest that microglia do not create Ox-stress at Aβ plaques but instead create protective barriers around Aβ plaques possibly reducing the spread of Aβ. Intracranial injection of Aβ was sufficient to induce neuronal Ox-stress suggesting it to be the initial trigger of Ox-stress generation. Although Ox-stress is increased in DNs, neuronal survival is enhanced following microglia depletion indicating complex and multifactorial roles of microglia with both neurotoxic and neuroprotective components. Increased Ox-stress of DNs was correlated with higher LAMP1 and ubiquitin immunoreactivity supporting proposed mechanistic links between lysosomal accumulation in DNs and their intrinsic generation of Ox-stress. Our results suggest protective as well as neurotoxic roles for microglia at plaques and that the generation of Ox-stress of DNs could intrinsically be generated via lysosomal disruption rather than by microglia. In Brief: Simultaneous imaging of microglia and neuronal Ox-stress revealed a double-edged role for microglia in 5xFAD mice. Plaque associated microglia were attracted to and enwrapped Aβ plaques as well as the most highly oxidized DNs. After partial depletion of microglia, DNs were larger with greater levels of Ox-stress. Despite increased Ox-stress after microglia removal neuronal survival improved. Greater Ox-stress was correlated with increased levels of LAMP1 and ubiquitin thereby linking lysosome accumulation and Ox-stress in DNs." @default.
• W4292805493 created "2022-08-24" @default.
• W4292805493 creator A5002354827 @default.
• W4292805493 creator A5005787962 @default.
• W4292805493 creator A5015911518 @default.
• W4292805493 creator A5030192413 @default.
• W4292805493 creator A5038558853 @default.
• W4292805493 creator A5042667110 @default.
• W4292805493 creator A5046548037 @default.
• W4292805493 creator A5055955887 @default.
• W4292805493 creator A5061451539 @default.
• W4292805493 creator A5081104947 @default.
• W4292805493 date "2022-10-01" @default.
• W4292805493 modified "2023-10-18" @default.
• W4292805493 title "Simultaneous imaging of redox states in dystrophic neurites and microglia at Aβ plaques indicate lysosome accumulation not microglia correlate with increased oxidative stress" @default.
• W4292805493 cites W111731671 @default.
• W4292805493 cites W1551381188 @default.
• W4292805493 cites W1559197028 @default.
• W4292805493 cites W1878700164 @default.
• W4292805493 cites W1920357305 @default.
• W4292805493 cites W1969129718 @default.
• W4292805493 cites W1970498241 @default.
• W4292805493 cites W1976357608 @default.
• W4292805493 cites W1990752558 @default.
• W4292805493 cites W1991709390 @default.
• W4292805493 cites W1997161797 @default.
• W4292805493 cites W2001428953 @default.
• W4292805493 cites W2004389431 @default.
• W4292805493 cites W2008008775 @default.
• W4292805493 cites W2011982570 @default.
• W4292805493 cites W2019845863 @default.
• W4292805493 cites W2020592632 @default.
• W4292805493 cites W2021969592 @default.
• W4292805493 cites W2033758935 @default.
• W4292805493 cites W2035299293 @default.
• W4292805493 cites W2037627674 @default.
• W4292805493 cites W2048718653 @default.
• W4292805493 cites W2069248995 @default.
• W4292805493 cites W2069271483 @default.
• W4292805493 cites W2070159291 @default.
• W4292805493 cites W2082414904 @default.
• W4292805493 cites W2083355844 @default.
• W4292805493 cites W2083846733 @default.
• W4292805493 cites W2084593700 @default.
• W4292805493 cites W2086831255 @default.
• W4292805493 cites W2087243986 @default.
• W4292805493 cites W2123951949 @default.
• W4292805493 cites W2124490243 @default.
• W4292805493 cites W2128613449 @default.
• W4292805493 cites W2130755020 @default.
• W4292805493 cites W2132376565 @default.
• W4292805493 cites W2139976280 @default.
• W4292805493 cites W2161057248 @default.
• W4292805493 cites W2170644095 @default.
• W4292805493 cites W2281702574 @default.
• W4292805493 cites W2297442499 @default.
• W4292805493 cites W2297848350 @default.
• W4292805493 cites W2319548839 @default.
• W4292805493 cites W2408084759 @default.
• W4292805493 cites W2568450381 @default.
• W4292805493 cites W2586389990 @default.
• W4292805493 cites W2610500410 @default.
• W4292805493 cites W2743428214 @default.
• W4292805493 cites W2747687773 @default.
• W4292805493 cites W2764165275 @default.
• W4292805493 cites W2766388141 @default.
• W4292805493 cites W2766770190 @default.
• W4292805493 cites W2774947569 @default.
• W4292805493 cites W2778881642 @default.
• W4292805493 cites W2888292224 @default.
• W4292805493 cites W2891010257 @default.
• W4292805493 cites W2905266332 @default.
• W4292805493 cites W2913269710 @default.
• W4292805493 cites W2925097021 @default.
• W4292805493 cites W2946033659 @default.
• W4292805493 cites W2948343247 @default.
• W4292805493 cites W2964874449 @default.
• W4292805493 cites W2967683178 @default.
• W4292805493 cites W2969940182 @default.
• W4292805493 cites W2998819749 @default.
• W4292805493 cites W3047229149 @default.
• W4292805493 cites W3048426432 @default.
• W4292805493 cites W3084402113 @default.
• W4292805493 cites W3108616759 @default.
• W4292805493 cites W3111509171 @default.
• W4292805493 cites W3130500589 @default.
• W4292805493 cites W3177013726 @default.
• W4292805493 cites W4205876365 @default.
• W4292805493 cites W4281616414 @default.
• W4292805493 cites W584997619 @default.
• W4292805493 doi "https://doi.org/10.1016/j.redox.2022.102448" @default.
• W4292805493 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36037587" @default.
• W4292805493 hasPublicationYear "2022" @default.
• W4292805493 type Work @default.
• W4292805493 citedByCount "2" @default.
• W4292805493 countsByYear W42928054932023 @default.
• W4292805493 crossrefType "journal-article" @default.
• W4292805493 hasAuthorship W4292805493A5002354827 @default.

• next