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- W4292870313 abstract "Sclerotinia sclerotiorum is a notorious fungal pathogen with a broad host range, including many important crops. A previous study showed dill seed essential oil (DSEO) could inhibit S sclerotiorum pathogenicity and protect canola production. However, the molecular basis of DSEO anti-fungal activity is still not well studied. To investigate the mechanism of DSEO anti-fungal activity, RNA-sequencing was employed to identify differentially expressed genes (DEGs) of S sclerotiorum in response to DSEO treatment. A total of 2470, 3218, and 3793 DEGs were identified in S sclerotiorum after being treated by DSEO for 0.5, 1, and 2 h, respectively. These genes that express changes in the early stage are more likely affected directly by DSEO. Gene Ontology (GO) analysis revealed that these genes were mainly related to transmembrane transport, cell membrane, ribosome biogenesis, and proteasome complex. DSEO treatment primarily affected the membrane part of the fungal cell, particularly the endoplasmic reticulum (ER) membrane at 0.5 and 1-hour treatment. In addition, a bunch of DEGs associated with the proteasome pathway was markedly enriched at 2 h of treatment. It is speculated that DSEO achieves antifungal effects by influencing these targets or pathways. The information obtained in this study expanded the understanding of the antifungal mechanism of DSEO and enriched the resources available for interpreting its mechanism at molecular level." @default.
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- W4292870313 date "2022-08-01" @default.
- W4292870313 modified "2023-09-30" @default.
- W4292870313 title "Transcriptome Analysis Reveals the Mechanism of dill Seed Essential oil Against <i>Sclerotinia sclerotiorum</i>" @default.
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- W4292870313 doi "https://doi.org/10.1177/1934578x221119910" @default.
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