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- W4292976656 abstract "Abstract Frog virus 3 (FV3, genera Ranavirus , family Iridoviridae ), a double-stranded DNA virus, results in irreparable damage to biodiversity and significant economic losses to aquaculture. Although the existing FV3 detection methods are of high sensitivity and specificity, the complex procedure and requirement of expensive instruments limit their practical implantation. Herein, we develop a fast, easy-to-implement, highly sensitive, and point-of-care (POC) detection system for FV3. Combining recombinase polymerase amplification (RPA) and CRISPR/Cas12a, we achieve a limit of detection (LoD) of 100 aM (60.2 copies/μL) by optimizing RPA primers and CRISPR RNAs (crRNAs). For POC detection, we build a smartphone microscopy (SPM) and achieve an LoD of 10 aM within 40 minutes. Four positive animal-derived samples with a quantitation cycle (Cq) value of quantitative PCR (qPCR) in the range of 13 to 32 are detectable by the proposed system. In addition, we deploy deep learning models for binary classification (positive or negative samples) and multiclass classification (different concentrations of FV3 and negative samples), achieving 100% and 98.75% accuracy, respectively. Without temperature regulation and expensive equipment, RPA-CRISPR/Cas12a combined with a smartphone readout and artificial intelligence (AI) assisted classification shows great potential for FV3 detection. This integrated system holds great promise for POC detection of aquatic DNA pathogens. Highlights An integrated DNA detection system is developed by combining RPA, CRISPR/Cas12a, smartphone microscopy, and deep learning. The LoD of frog virus 3 is 10 aM within 40 min. The detection system shows good performance on animal-derived samples." @default.
- W4292976656 created "2022-08-24" @default.
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- W4292976656 date "2022-08-22" @default.
- W4292976656 modified "2023-09-27" @default.
- W4292976656 title "Detection of Frog virus 3 via the system integrating RPA-CRISPR/Cas12a-SPM with deep learning" @default.
- W4292976656 cites W1594568422 @default.
- W4292976656 cites W1970117208 @default.
- W4292976656 cites W1981322664 @default.
- W4292976656 cites W1985649510 @default.
- W4292976656 cites W1986316308 @default.
- W4292976656 cites W2016635701 @default.
- W4292976656 cites W2040763658 @default.
- W4292976656 cites W2053786596 @default.
- W4292976656 cites W2063505016 @default.
- W4292976656 cites W2066389586 @default.
- W4292976656 cites W2098534337 @default.
- W4292976656 cites W2105137612 @default.
- W4292976656 cites W2162676125 @default.
- W4292976656 cites W2165758815 @default.
- W4292976656 cites W2165869860 @default.
- W4292976656 cites W2342161390 @default.
- W4292976656 cites W2571312154 @default.
- W4292976656 cites W2584810127 @default.
- W4292976656 cites W2606915822 @default.
- W4292976656 cites W2646182870 @default.
- W4292976656 cites W2750082507 @default.
- W4292976656 cites W2766989075 @default.
- W4292976656 cites W2787024379 @default.
- W4292976656 cites W2790313560 @default.
- W4292976656 cites W2794807741 @default.
- W4292976656 cites W2808064229 @default.
- W4292976656 cites W2909146242 @default.
- W4292976656 cites W2920964100 @default.
- W4292976656 cites W2950557219 @default.
- W4292976656 cites W2950765969 @default.
- W4292976656 cites W2953698135 @default.
- W4292976656 cites W2963056293 @default.
- W4292976656 cites W2963647538 @default.
- W4292976656 cites W2967373993 @default.
- W4292976656 cites W2973445868 @default.
- W4292976656 cites W2981597206 @default.
- W4292976656 cites W2989441546 @default.
- W4292976656 cites W2994990179 @default.
- W4292976656 cites W3004806486 @default.
- W4292976656 cites W3006285715 @default.
- W4292976656 cites W3011496369 @default.
- W4292976656 cites W3015163530 @default.
- W4292976656 cites W3017334101 @default.
- W4292976656 cites W3038540011 @default.
- W4292976656 cites W3043350800 @default.
- W4292976656 cites W3087209706 @default.
- W4292976656 cites W3090132798 @default.
- W4292976656 cites W3092974990 @default.
- W4292976656 cites W3095698970 @default.
- W4292976656 cites W3095922471 @default.
- W4292976656 cites W3095986980 @default.
- W4292976656 cites W3097848659 @default.
- W4292976656 cites W3104138698 @default.
- W4292976656 cites W3108711749 @default.
- W4292976656 cites W3112814902 @default.
- W4292976656 cites W3121646766 @default.
- W4292976656 cites W3135005580 @default.
- W4292976656 cites W3156859314 @default.
- W4292976656 cites W3157675340 @default.
- W4292976656 cites W3159703094 @default.
- W4292976656 cites W3160593167 @default.
- W4292976656 cites W3160936377 @default.
- W4292976656 cites W3167725174 @default.
- W4292976656 cites W3169981325 @default.
- W4292976656 cites W3171873561 @default.
- W4292976656 cites W3189845806 @default.
- W4292976656 cites W3195277331 @default.
- W4292976656 cites W3203784909 @default.
- W4292976656 cites W3204082020 @default.
- W4292976656 cites W3206154855 @default.
- W4292976656 cites W3212726695 @default.
- W4292976656 cites W3217392944 @default.
- W4292976656 cites W4207003534 @default.
- W4292976656 cites W4212940964 @default.
- W4292976656 cites W4224947680 @default.
- W4292976656 cites W4281628509 @default.