Matches in SemOpenAlex for { <https://semopenalex.org/work/W4293004061> ?p ?o ?g. }
- W4293004061 endingPage "3161" @default.
- W4293004061 startingPage "3149" @default.
- W4293004061 abstract "Abstract Microorganisms build fatty acids with biocatalytic assembly lines, or fatty acid synthases (FASs), that can be repurposed to produce a broad set of fuels and chemicals. Despite their versatility, the product profiles of FAS‐based pathways are challenging to adjust without experimental iteration, and off‐target products are common. This study uses a detailed kinetic model of the Escherichia coli FAS as a foundation to model nine oleochemical pathways. These models provide good fits to experimental data and help explain unexpected results from in vivo studies. An analysis of pathways for alkanes and fatty acid ethyl esters (FAEEs), for example, suggests that reductions in titer caused by enzyme overexpression—an experimentally consistent phenomenon—can result from shifts in metabolite pools that are incompatible with the substrate specificities of downstream enzymes, and a focused examination of multiple alcohol pathways indicates that coordinated shifts in enzyme concentrations provide a general means of tuning the product profiles of pathways with promiscuous components. The study concludes by integrating all models into a graphical user interface. The models supplied by this work provide a versatile kinetic framework for studying oleochemical pathways in different biochemical contexts." @default.
- W4293004061 created "2022-08-25" @default.
- W4293004061 creator A5000477607 @default.
- W4293004061 creator A5016071275 @default.
- W4293004061 creator A5018728489 @default.
- W4293004061 creator A5028356555 @default.
- W4293004061 creator A5065352826 @default.
- W4293004061 date "2022-08-24" @default.
- W4293004061 modified "2023-09-26" @default.
- W4293004061 title "A kinetic framework for modeling oleochemical biosynthesis in <i>Escherichia coli</i>" @default.
- W4293004061 cites W1493623656 @default.
- W4293004061 cites W1535712589 @default.
- W4293004061 cites W1548129143 @default.
- W4293004061 cites W1581467588 @default.
- W4293004061 cites W1730769032 @default.
- W4293004061 cites W1757458658 @default.
- W4293004061 cites W1966903347 @default.
- W4293004061 cites W1968220057 @default.
- W4293004061 cites W1969847819 @default.
- W4293004061 cites W1975327876 @default.
- W4293004061 cites W1976257434 @default.
- W4293004061 cites W1977153758 @default.
- W4293004061 cites W2000150815 @default.
- W4293004061 cites W2019303832 @default.
- W4293004061 cites W2021266593 @default.
- W4293004061 cites W2021655853 @default.
- W4293004061 cites W2034788651 @default.
- W4293004061 cites W2038459381 @default.
- W4293004061 cites W2040634163 @default.
- W4293004061 cites W2041272070 @default.
- W4293004061 cites W2041618640 @default.
- W4293004061 cites W2047360542 @default.
- W4293004061 cites W2047527115 @default.
- W4293004061 cites W2047748350 @default.
- W4293004061 cites W2072916270 @default.
- W4293004061 cites W2074893062 @default.
- W4293004061 cites W2081121707 @default.
- W4293004061 cites W2086943750 @default.
- W4293004061 cites W2094858924 @default.
- W4293004061 cites W2102004106 @default.
- W4293004061 cites W2106095122 @default.
- W4293004061 cites W2142251632 @default.
- W4293004061 cites W2165182519 @default.
- W4293004061 cites W2213256540 @default.
- W4293004061 cites W2217457428 @default.
- W4293004061 cites W2223326830 @default.
- W4293004061 cites W2295813225 @default.
- W4293004061 cites W2326626972 @default.
- W4293004061 cites W2332358028 @default.
- W4293004061 cites W2342429517 @default.
- W4293004061 cites W2344128917 @default.
- W4293004061 cites W2418146040 @default.
- W4293004061 cites W2509228405 @default.
- W4293004061 cites W2522753993 @default.
- W4293004061 cites W2594348490 @default.
- W4293004061 cites W2603300152 @default.
- W4293004061 cites W2607155480 @default.
- W4293004061 cites W2766500735 @default.
- W4293004061 cites W2775766311 @default.
- W4293004061 cites W2790394089 @default.
- W4293004061 cites W2790456375 @default.
- W4293004061 cites W2791350401 @default.
- W4293004061 cites W2799698220 @default.
- W4293004061 cites W2800706370 @default.
- W4293004061 cites W2885377742 @default.
- W4293004061 cites W2885738694 @default.
- W4293004061 cites W2898724032 @default.
- W4293004061 cites W2900498998 @default.
- W4293004061 cites W2942090706 @default.
- W4293004061 cites W2967261210 @default.
- W4293004061 cites W3031696350 @default.
- W4293004061 cites W3044888145 @default.
- W4293004061 cites W3081574745 @default.
- W4293004061 cites W3119909118 @default.
- W4293004061 cites W3122203348 @default.
- W4293004061 cites W3134654075 @default.
- W4293004061 cites W3174303789 @default.
- W4293004061 cites W3204042087 @default.
- W4293004061 cites W3213751857 @default.
- W4293004061 cites W3216034917 @default.
- W4293004061 cites W4225939313 @default.
- W4293004061 cites W999207820 @default.
- W4293004061 doi "https://doi.org/10.1002/bit.28209" @default.
- W4293004061 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35959746" @default.
- W4293004061 hasPublicationYear "2022" @default.
- W4293004061 type Work @default.
- W4293004061 citedByCount "2" @default.
- W4293004061 countsByYear W42930040612023 @default.
- W4293004061 crossrefType "journal-article" @default.
- W4293004061 hasAuthorship W4293004061A5000477607 @default.
- W4293004061 hasAuthorship W4293004061A5016071275 @default.
- W4293004061 hasAuthorship W4293004061A5018728489 @default.
- W4293004061 hasAuthorship W4293004061A5028356555 @default.
- W4293004061 hasAuthorship W4293004061A5065352826 @default.
- W4293004061 hasBestOaLocation W42930040611 @default.
- W4293004061 hasConcept C104317684 @default.
- W4293004061 hasConcept C181199279 @default.
- W4293004061 hasConcept C185592680 @default.