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• W4293018348 abstract "first_page settings Order Article Reprints Font Type: Arial Georgia Verdana Font Size: Aa Aa Aa Line Spacing:    Column Width:    Background: Open AccessAbstract Electrochemical Biosensors for Cardiovascular Diseases’ (CVDs) Biomarkers Detection † by Daria Rudewicz-Kowalczyk and Iwona Grabowska * Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Tuwima 10, 10-748 Olsztyn, Poland * Author to whom correspondence should be addressed. † Presented at the 9th International Symposium on Sensor Science, Warsaw, Poland, 20–22 June 2022. Eng. Proc. 2022, 21(1), 11; https://doi.org/10.3390/engproc2022021011 Published: 23 August 2022 (This article belongs to the Proceedings of The 9th International Symposium on Sensor Science) Download Download PDF Download PDF with Cover Download XML Download Epub Versions Notes Cardiovascular diseases (CVDs), a group of diseases related to heart and blood vessel disorders, are the main health problem worldwide. Therefore, the development of modern diagnostic technologies for the rapid and accurate detection of cardiac biomarkers has recently become of high significance. Sensitive protein biomarker identification can help to prevent and slow down CVD progression. The ability of electrochemical biosensors to determine cardiovascular biomarkers, such as cardiac troponin I (cTnI), brain natriuretic peptide (BNP-32) and low-density lipoprotein (LDL), in the serum of patients is crucial for the development of point-of-care sensing platforms. Electrochemical biosensors, cheap and scalable devices, offer, moreover, attractive alternatives for conventional immunoassays. In our group, we joined this research line and, in this presentation, we report on:Electrochemical aptasensors for the detection of BNP-32 and cTnI using differential pulse voltammetry (DPV) in Phosphate buffer saline (PBS) and [Fe(CN)6]4−, based on screen-printed gold electrodes modified electrophoretically with (1) polyethyleneimine/reduced graphene films, (2) subsequently with propargylacetic acid, and (3) azide-terminated aptamers immobilized using Cu(I)-based “click” chemistry on propargyl groups;The comparison of electrochemical immuno- and aptasensor for the detection of LDL using square-wave voltammetry (SWV) in PBS containing [Fe(CN)6]3−/4−, an immunosensor based on anti-apolipoprotein B-100 antibody covalently attached to 4-aminothiophenol (4-ATP) on the surface of the gold electrode and an aptasensor based on the gold electrode modified in a mixture of 6-mercaptohexanol and ssDNA aptamer specific for the LDL-containing -SH group;Studies on the application of monoclonal antibodies against LDL labelled with redox-active molecules (derivatives of ferrocene) immobilized on the surface of the gold electrode for the detection of LDL based on changes in ferrocene redox activity. Author ContributionsExperimental electrochemical studies, data analysis and interpretation, collecting literature, writing—draft preparation, D.R.-K.; funding acquisition, conceptualization, methodology, supervision, writing—review and editing, I.G. All authors have read and agreed to the published version of the manuscript.FundingThis work was supported by the National Science Centre, Poland, grant number 2017/25/B/ST4/00139 and Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, in Olsztyn.Conflicts of InterestThe authors declare no conflict of interest.Publisher’s Note: MDPI stays neutral with regard to jurisdictional claims in published maps and institutional affiliations. © 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Share and Cite MDPI and ACS Style Rudewicz-Kowalczyk, D.; Grabowska, I. Electrochemical Biosensors for Cardiovascular Diseases’ (CVDs) Biomarkers Detection. Eng. Proc. 2022, 21, 11. https://doi.org/10.3390/engproc2022021011 AMA Style Rudewicz-Kowalczyk D, Grabowska I. Electrochemical Biosensors for Cardiovascular Diseases’ (CVDs) Biomarkers Detection. Engineering Proceedings. 2022; 21(1):11. https://doi.org/10.3390/engproc2022021011 Chicago/Turabian Style Rudewicz-Kowalczyk, Daria, and Iwona Grabowska. 2022. Electrochemical Biosensors for Cardiovascular Diseases’ (CVDs) Biomarkers Detection Engineering Proceedings 21, no. 1: 11. https://doi.org/10.3390/engproc2022021011 Find Other Styles Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here. Article Metrics No No Article Access Statistics Multiple requests from the same IP address are counted as one view." @default.
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