FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4293207296> ?p ?o ?g. }

• W4293207296 abstract "Implanted biomaterials elicit an immune-mediated foreign body reaction (FBR) that results in the fibrous encapsulation of the implant and can critically impact the performance of some implants. Consequently, understanding the molecular mechanisms that underpin cell-materials interactions that initiate biomaterial-induced inflammation and fibrosis is critical to improving the performance of biomaterial implants negatively impacted by the FBR. Damage-associated molecular patterns (DAMPs) are endogenous mediators of inflammation that are released upon tissue injury and induce sterile inflammation via Toll-like receptors (TLRs). However, the prevalence of DAMPs within the adsorbed protein layer on material surfaces and their role mediating cell-material interactions is unclear. Previously, our group demonstrated that molecules in fibroblast lysates adsorbed to various biomaterials and induced a potent TLR2-dependent inflammatory response in macrophages at 24 h. In this study, we examined the extended response of RAW-Blue reporter macrophages on lysate or serum-adsorbed Teflon™ AF surfaces to understand the potential role of adsorbed DAMPs in macrophage-material interactions at later time points. Lysate-conditioned surfaces maintained increased nuclear factor kappa B (NF-κB) and activator protein 1 (AP-1) transcription factor activity and increased expression Regulated upon Activation, Normal T Cell Expressed and Presumably Secreted (RANTES/CCL5) at 72 h and 120 h, compared to FBS-conditioned surfaces. In contrast, monocyte chemoattractant protein 1 (MCP-1/CCL2) was only elevated at 72 h in lysate conditions. Transforming growth factor beta 1 (TGF-β1) secretion was significantly increased on lysate-conditioned surfaces, while conditioned media from macrophages on lysate-conditioned surfaces induced alpha smooth muscle actin (αSMA) expression in 3T3 fibroblasts. TLR2 neutralizing antibody treatment significantly decreased NF-κB/AP-1 activity and attenuated TGF-β1 expression at both time points, and MCP-1 and RANTES at 72 h. Finally, multinucleated cells were observed on lysate-conditioned surfaces at 72 h, indicating adsorbed DAMPs induced a fusion permissive environment for adherent macrophages. This study demonstrates that adsorbed DAMPs continue to influence macrophage-material responses beyond the initial 24-h period and maintain a pro-inflammatory and fibrotic response that models aspects of the early FBR. Furthermore, the transient inhibition of TLR2 continued to exert an effect at these later time points, suggesting TLR2 may be a target for therapeutic interventions in FBR." @default.
• W4293207296 created "2022-08-27" @default.
• W4293207296 creator A5012796768 @default.
• W4293207296 creator A5049414246 @default.
• W4293207296 creator A5061353550 @default.
• W4293207296 creator A5088453668 @default.
• W4293207296 date "2022-08-26" @default.
• W4293207296 modified "2023-10-06" @default.
• W4293207296 title "The extended effect of adsorbed damage-associated molecular patterns and Toll-like receptor 2 signaling on macrophage-material interactions" @default.
• W4293207296 cites W1512269824 @default.
• W4293207296 cites W1574776624 @default.
• W4293207296 cites W1615432648 @default.
• W4293207296 cites W1791734915 @default.
• W4293207296 cites W1827936963 @default.
• W4293207296 cites W1849923365 @default.
• W4293207296 cites W1966562602 @default.
• W4293207296 cites W1974577462 @default.
• W4293207296 cites W1974878015 @default.
• W4293207296 cites W1976789092 @default.
• W4293207296 cites W1986752194 @default.
• W4293207296 cites W1995063875 @default.
• W4293207296 cites W1998415432 @default.
• W4293207296 cites W2002834668 @default.
• W4293207296 cites W2011811777 @default.
• W4293207296 cites W2013844344 @default.
• W4293207296 cites W2016564842 @default.
• W4293207296 cites W2018202875 @default.
• W4293207296 cites W2020087937 @default.
• W4293207296 cites W2021703335 @default.
• W4293207296 cites W2027427234 @default.
• W4293207296 cites W2028314282 @default.
• W4293207296 cites W2031375672 @default.
• W4293207296 cites W2036077057 @default.
• W4293207296 cites W2051651031 @default.
• W4293207296 cites W2053236659 @default.
• W4293207296 cites W2053837765 @default.
• W4293207296 cites W2055348159 @default.
• W4293207296 cites W2056336363 @default.
• W4293207296 cites W2058522210 @default.
• W4293207296 cites W2061609938 @default.
• W4293207296 cites W2076336189 @default.
• W4293207296 cites W2079236567 @default.
• W4293207296 cites W2088176919 @default.
• W4293207296 cites W2099540110 @default.
• W4293207296 cites W2099944930 @default.
• W4293207296 cites W2110768898 @default.
• W4293207296 cites W2112055131 @default.
• W4293207296 cites W2127236332 @default.
• W4293207296 cites W2134219545 @default.
• W4293207296 cites W2171177609 @default.
• W4293207296 cites W2179951052 @default.
• W4293207296 cites W2197489357 @default.
• W4293207296 cites W2230207762 @default.
• W4293207296 cites W2338762173 @default.
• W4293207296 cites W2546645949 @default.
• W4293207296 cites W2550993130 @default.
• W4293207296 cites W2567221680 @default.
• W4293207296 cites W2605242173 @default.
• W4293207296 cites W2610090004 @default.
• W4293207296 cites W2735527928 @default.
• W4293207296 cites W2752337765 @default.
• W4293207296 cites W27606866 @default.
• W4293207296 cites W2762466554 @default.
• W4293207296 cites W2784126038 @default.
• W4293207296 cites W2786007302 @default.
• W4293207296 cites W2790821500 @default.
• W4293207296 cites W2791461007 @default.
• W4293207296 cites W2798093954 @default.
• W4293207296 cites W2806376977 @default.
• W4293207296 cites W2807629723 @default.
• W4293207296 cites W2861717878 @default.
• W4293207296 cites W2887043640 @default.
• W4293207296 cites W2899539894 @default.
• W4293207296 cites W2900826101 @default.
• W4293207296 cites W2910745688 @default.
• W4293207296 cites W2914708864 @default.
• W4293207296 cites W2971483210 @default.
• W4293207296 cites W2976534380 @default.
• W4293207296 cites W2981180699 @default.
• W4293207296 cites W2993943329 @default.
• W4293207296 cites W2999318389 @default.
• W4293207296 cites W3004951738 @default.
• W4293207296 cites W3006804697 @default.
• W4293207296 cites W3112713493 @default.
• W4293207296 cites W3128384071 @default.
• W4293207296 cites W3128592268 @default.
• W4293207296 cites W3146582890 @default.
• W4293207296 cites W3154658958 @default.
• W4293207296 cites W3158686773 @default.
• W4293207296 cites W3178890732 @default.
• W4293207296 cites W3215304907 @default.
• W4293207296 cites W3216086757 @default.
• W4293207296 cites W4212766892 @default.
• W4293207296 cites W4214770922 @default.
• W4293207296 cites W4220918277 @default.
• W4293207296 cites W4238125128 @default.
• W4293207296 doi "https://doi.org/10.3389/fbioe.2022.959512" @default.
• W4293207296 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36091432" @default.
• W4293207296 hasPublicationYear "2022" @default.
• W4293207296 type Work @default.

• next