FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4293219005> ?p ?o ?g. }

• W4293219005 endingPage "163" @default.
• W4293219005 startingPage "156" @default.
• W4293219005 abstract "Nerve Growth Factor (NGF) is a signalling molecule for pain and inflammation. NGF is increased in synovial fluid from osteoarthritic humans and animals, compared to healthy controls. Monoclonal antibody therapy directed against NGF has been approved to treat pain in osteoarthritic dogs but despite many years of trialling, therapy has not been approved for human use. One reason for this is that adverse reactions with rapidly progressing osteoarthritis has occurred in some individuals. More detailed knowledge of NGF expression in joints is needed. In this study, capillary-based Simple Western was used to analyse NGF in cultured equine chondrocytes. Chondrocytes were collected post mortem from three macroscopically healthy intercarpal joints and three intercarpal joints with mild osteoarthritic changes. The chondrocytes were expanded to passage one and seeded in chondrogenic medium to maintain the phenotype. On day four, cells were either stimulated with LPS or kept untreated in medium. All cells were harvested on day five. Wes analysis of lysates did not show mature NGF but two proforms, 40 and 45 kDa, were identified. Results were confirmed with western blot. The same proforms were expressed in chondrocytes from healthy and osteoarthritic joints. Acute inflammation induced by LPS stimulation did not change the forms of expressed NGF. Capillary Simple Western offers a sensitive and sample-sparing alternative to traditional western blot. However, confirmation of peaks is imperative in order to avoid misinterpretation of findings. In addition, in this case the method did not offer the possibility of quantification advertised by the manufacturers." @default.
• W4293219005 created "2022-08-27" @default.
• W4293219005 creator A5038842105 @default.
• W4293219005 creator A5047936615 @default.
• W4293219005 creator A5056553098 @default.
• W4293219005 creator A5070663898 @default.
• W4293219005 creator A5083525010 @default.
• W4293219005 date "2022-12-01" @default.
• W4293219005 modified "2023-09-26" @default.
• W4293219005 title "The expression of nerve growth factor in healthy and inflamed equine chondrocytes analysed by capillary western immunoassay" @default.
• W4293219005 cites W1185447612 @default.
• W4293219005 cites W1558635903 @default.
• W4293219005 cites W1565654706 @default.
• W4293219005 cites W1593538742 @default.
• W4293219005 cites W1880716631 @default.
• W4293219005 cites W1963541881 @default.
• W4293219005 cites W1969349734 @default.
• W4293219005 cites W1971200702 @default.
• W4293219005 cites W1979499962 @default.
• W4293219005 cites W1984179481 @default.
• W4293219005 cites W1986121436 @default.
• W4293219005 cites W1989588869 @default.
• W4293219005 cites W2014256027 @default.
• W4293219005 cites W2038091223 @default.
• W4293219005 cites W2070297491 @default.
• W4293219005 cites W2082082888 @default.
• W4293219005 cites W2087090756 @default.
• W4293219005 cites W2088903390 @default.
• W4293219005 cites W2092026744 @default.
• W4293219005 cites W2119108332 @default.
• W4293219005 cites W2151190673 @default.
• W4293219005 cites W2160803929 @default.
• W4293219005 cites W2165471060 @default.
• W4293219005 cites W2330956224 @default.
• W4293219005 cites W2418138502 @default.
• W4293219005 cites W2495327668 @default.
• W4293219005 cites W2515610907 @default.
• W4293219005 cites W2592653867 @default.
• W4293219005 cites W2593689031 @default.
• W4293219005 cites W2609354761 @default.
• W4293219005 cites W2612221856 @default.
• W4293219005 cites W2729676784 @default.
• W4293219005 cites W2754407077 @default.
• W4293219005 cites W2754943482 @default.
• W4293219005 cites W2797800095 @default.
• W4293219005 cites W2899075740 @default.
• W4293219005 cites W2976103322 @default.
• W4293219005 cites W3014194256 @default.
• W4293219005 cites W3106689419 @default.
• W4293219005 cites W3200220451 @default.
• W4293219005 cites W4280620758 @default.
• W4293219005 doi "https://doi.org/10.1016/j.rvsc.2022.08.015" @default.
• W4293219005 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36029605" @default.
• W4293219005 hasPublicationYear "2022" @default.
• W4293219005 type Work @default.
• W4293219005 citedByCount "1" @default.
• W4293219005 countsByYear W42932190052023 @default.
• W4293219005 crossrefType "journal-article" @default.
• W4293219005 hasAuthorship W4293219005A5038842105 @default.
• W4293219005 hasAuthorship W4293219005A5047936615 @default.
• W4293219005 hasAuthorship W4293219005A5056553098 @default.
• W4293219005 hasAuthorship W4293219005A5070663898 @default.
• W4293219005 hasAuthorship W4293219005A5083525010 @default.
• W4293219005 hasBestOaLocation W42932190051 @default.
• W4293219005 hasConcept C104317684 @default.
• W4293219005 hasConcept C105702510 @default.
• W4293219005 hasConcept C126322002 @default.
• W4293219005 hasConcept C142724271 @default.
• W4293219005 hasConcept C159654299 @default.
• W4293219005 hasConcept C16685009 @default.
• W4293219005 hasConcept C170493617 @default.
• W4293219005 hasConcept C185592680 @default.
• W4293219005 hasConcept C203014093 @default.
• W4293219005 hasConcept C204787440 @default.
• W4293219005 hasConcept C2776164576 @default.
• W4293219005 hasConcept C2776268809 @default.
• W4293219005 hasConcept C2776415932 @default.
• W4293219005 hasConcept C2776914184 @default.
• W4293219005 hasConcept C2778423431 @default.
• W4293219005 hasConcept C2780420688 @default.
• W4293219005 hasConcept C2780550940 @default.
• W4293219005 hasConcept C35496256 @default.
• W4293219005 hasConcept C542903549 @default.
• W4293219005 hasConcept C55493867 @default.
• W4293219005 hasConcept C71924100 @default.
• W4293219005 hasConcept C85265743 @default.
• W4293219005 hasConceptScore W4293219005C104317684 @default.
• W4293219005 hasConceptScore W4293219005C105702510 @default.
• W4293219005 hasConceptScore W4293219005C126322002 @default.
• W4293219005 hasConceptScore W4293219005C142724271 @default.
• W4293219005 hasConceptScore W4293219005C159654299 @default.
• W4293219005 hasConceptScore W4293219005C16685009 @default.
• W4293219005 hasConceptScore W4293219005C170493617 @default.
• W4293219005 hasConceptScore W4293219005C185592680 @default.
• W4293219005 hasConceptScore W4293219005C203014093 @default.
• W4293219005 hasConceptScore W4293219005C204787440 @default.
• W4293219005 hasConceptScore W4293219005C2776164576 @default.
• W4293219005 hasConceptScore W4293219005C2776268809 @default.

• next