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- W4293247539 abstract "Abstract RNA modifications play pivotal roles in numerous cellular processes and human diseases. In addition to well-studied methyl-based modifications, hydrophobic prenyl-modifications have also been found in many RNA species. Here we report two chemical labeling strategies for tagging lipid-modified RNAs by taking advantage of a natural SelU-mediated tRNA geranylation process and the special reactivity of prenyl-groups. We synthesized a series of ‘clickable’ geranyl-pyrophosphate analogs and identified two candidates for indirect RNA labeling using a two-step process, a zidation-and- c lick- t agging of flu orescent dyes, namely ACT-Flu. We also developed a direct m etabolic i ncorporation and b iorthogonal tagging (MIBT-Tag) method based on the Ene-ligation of prenyl-groups. Both methods have been successfully applied to in-cell RNA labeling and the identification of new proteins associated with the geranylation process through proteomic and bioinformatic studies. These biochemical toolsets enable further in vivo applications to study prenylation pathways and monitor their status in both healthy and diseased cells." @default.
- W4293247539 created "2022-08-27" @default.
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- W4293247539 date "2022-05-17" @default.
- W4293247539 modified "2023-09-22" @default.
- W4293247539 title "Nature-Inspired Chemical Probes for in-cell Labeling of Lipidized RNA and Identification of New Regulatory Enzymes" @default.
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- W4293247539 doi "https://doi.org/10.1101/2022.05.17.492276" @default.
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