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Matches in SemOpenAlex for { <https://semopenalex.org/work/W4293256810> ?p ?o ?g. }

• W4293256810 endingPage "128949" @default.
• W4293256810 startingPage "128949" @default.
• W4293256810 abstract "Abnormal microRNA (miRNA) expression levels are confirmed as diagnostic biomarkers of the emergence and development of diseases. In this study, we developed a fluorescence biosensor for detecting miRNAs based on double amplification reactions with the primer exchange reaction (PER) and CRISPR/Cas12a. In the absence of target miRNA-21, PER hairpins remained locked by the protector strands and the primers did not extend. In the presence of target miRNA-21, the miRNA-21 bound to the guard sequence and exposed primer binding sites. Also, the closed PER hairpin was unlocked to specifically extend primers into single-stranded DNA (ssDNA) of unequal lengths. These ssDNAs of unequal lengths could activate the cleavage of a reporter by Cas12a, leading to an increase in detectable fluorescence signals. A large number of short nucleic acid fragments were amplified by PER-CRISPR multiple cycle cleavage fluorescent probes. Based on PER-combined CRISPR/Cas12a established dual signal amplification method was characterized by a low limit of detection of 10fM. The fluorescent biosensor for miRNA detection had the advantages of low detection cost, simple operation, and mobility, providing a very promising platform for the point-of-care testing of miRNA-21." @default.
• W4293256810 created "2022-08-27" @default.
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• W4293256810 date "2022-10-01" @default.
• W4293256810 modified "2023-10-16" @default.
• W4293256810 title "Ultrasensitive, rapid, and highly specific detection of microRNAs based on PER-CRISPR/CAS" @default.
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• W4293256810 doi "https://doi.org/10.1016/j.bmcl.2022.128949" @default.
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• W4293256810 hasPublicationYear "2022" @default.
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