Matches in SemOpenAlex for { <https://semopenalex.org/work/W4293456941> ?p ?o ?g. }
- W4293456941 endingPage "3876" @default.
- W4293456941 startingPage "3869" @default.
- W4293456941 abstract "The carboxyl groups of a protein can be esterified by reaction with a diazo compound, 2-diazo-2-(p-methylphenyl)-N,N-dimethylacetamide. This esterification enables the entry of the protein into the cytosol of a mammalian cell, where the nascent ester groups are hydrolyzed by endogenous esterases. The low aqueous solubility of the ensuing esterified protein is, however, a major practical challenge. Solubility screening revealed that β-cyclodextrin (β-CD) is an optimal solubilizing agent for esterified green fluorescent protein (est-GFP). Its addition can increase the recovery of est-GFP by 10-fold. α-CD, γ-CD, and cucurbit-7-uril are less effective excipients. 1H NMR titration experiments revealed that β-CD encapsulates the hydrophobic tolyl group of ester conjugates with Ka = 321 M-1. Combining l-arginine and sucrose with β-CD enables the nearly quantitative recovery of est-GFP. Thus, the insolubility of esterified proteins can be overcome with excipients." @default.
- W4293456941 created "2022-08-29" @default.
- W4293456941 creator A5019213891 @default.
- W4293456941 creator A5048283562 @default.
- W4293456941 creator A5071053466 @default.
- W4293456941 creator A5078253624 @default.
- W4293456941 date "2022-08-29" @default.
- W4293456941 modified "2023-10-17" @default.
- W4293456941 title "Host–Guest Complexation by β-Cyclodextrin Enhances the Solubility of an Esterified Protein" @default.
- W4293456941 cites W1496512127 @default.
- W4293456941 cites W1562907760 @default.
- W4293456941 cites W1569329587 @default.
- W4293456941 cites W1576271719 @default.
- W4293456941 cites W1582756289 @default.
- W4293456941 cites W1731708084 @default.
- W4293456941 cites W1836923993 @default.
- W4293456941 cites W1901529859 @default.
- W4293456941 cites W1909661131 @default.
- W4293456941 cites W1964839527 @default.
- W4293456941 cites W1982762997 @default.
- W4293456941 cites W1984140592 @default.
- W4293456941 cites W1987929665 @default.
- W4293456941 cites W1989378029 @default.
- W4293456941 cites W1991447915 @default.
- W4293456941 cites W1993375177 @default.
- W4293456941 cites W1995120802 @default.
- W4293456941 cites W2007584726 @default.
- W4293456941 cites W2009136136 @default.
- W4293456941 cites W2011881868 @default.
- W4293456941 cites W2017084486 @default.
- W4293456941 cites W2020337784 @default.
- W4293456941 cites W2028697236 @default.
- W4293456941 cites W2029779460 @default.
- W4293456941 cites W2036704607 @default.
- W4293456941 cites W2038422254 @default.
- W4293456941 cites W2046017608 @default.
- W4293456941 cites W2047168931 @default.
- W4293456941 cites W2048061357 @default.
- W4293456941 cites W2048312152 @default.
- W4293456941 cites W2051776626 @default.
- W4293456941 cites W2053797549 @default.
- W4293456941 cites W2056017788 @default.
- W4293456941 cites W2057127239 @default.
- W4293456941 cites W2059142487 @default.
- W4293456941 cites W2067848480 @default.
- W4293456941 cites W2070509328 @default.
- W4293456941 cites W2071066604 @default.
- W4293456941 cites W2077215811 @default.
- W4293456941 cites W2083811444 @default.
- W4293456941 cites W2087579391 @default.
- W4293456941 cites W2088325661 @default.
- W4293456941 cites W2090542622 @default.
- W4293456941 cites W2115024060 @default.
- W4293456941 cites W2120894192 @default.
- W4293456941 cites W2131678310 @default.
- W4293456941 cites W2131692566 @default.
- W4293456941 cites W2135868701 @default.
- W4293456941 cites W2136310012 @default.
- W4293456941 cites W2141654481 @default.
- W4293456941 cites W2142447133 @default.
- W4293456941 cites W2143253342 @default.
- W4293456941 cites W2162651138 @default.
- W4293456941 cites W2164026904 @default.
- W4293456941 cites W2168007081 @default.
- W4293456941 cites W2230749088 @default.
- W4293456941 cites W2290605832 @default.
- W4293456941 cites W2331741530 @default.
- W4293456941 cites W2521122533 @default.
- W4293456941 cites W2745164897 @default.
- W4293456941 cites W2763313852 @default.
- W4293456941 cites W2809722643 @default.
- W4293456941 cites W2919253225 @default.
- W4293456941 cites W2923551262 @default.
- W4293456941 cites W3081311906 @default.
- W4293456941 cites W3148925755 @default.
- W4293456941 cites W4234944446 @default.
- W4293456941 doi "https://doi.org/10.1021/acs.molpharmaceut.2c00368" @default.
- W4293456941 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36036888" @default.
- W4293456941 hasPublicationYear "2022" @default.
- W4293456941 type Work @default.
- W4293456941 citedByCount "2" @default.
- W4293456941 countsByYear W42934569412023 @default.
- W4293456941 crossrefType "journal-article" @default.
- W4293456941 hasAuthorship W4293456941A5019213891 @default.
- W4293456941 hasAuthorship W4293456941A5048283562 @default.
- W4293456941 hasAuthorship W4293456941A5071053466 @default.
- W4293456941 hasAuthorship W4293456941A5078253624 @default.
- W4293456941 hasBestOaLocation W42934569412 @default.
- W4293456941 hasConcept C104317684 @default.
- W4293456941 hasConcept C142613039 @default.
- W4293456941 hasConcept C155574463 @default.
- W4293456941 hasConcept C178790620 @default.
- W4293456941 hasConcept C184651966 @default.
- W4293456941 hasConcept C184866935 @default.
- W4293456941 hasConcept C185592680 @default.
- W4293456941 hasConcept C21951064 @default.
- W4293456941 hasConcept C2776904781 @default.
- W4293456941 hasConcept C2779433975 @default.