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- W4293491589 abstract "Phage-encoded homologous recombination (PEHR) is an efficient tool for bacterial genome editing. We previously developed and utilized a Pseudomonas-specific PEHR system. However, when using the PEHR system for Pseudomonas genome editing, false positives can be a problem. In this study, we combined a compact Cascade-Cas3 system from P. aeruginosa (PaeCas3c) with a Pseudomonas-specific PEHR system, and the results of our recombineering assay showed that this compact Cascade-Cas3 system can significantly improve PEHR recombineering accuracy." @default.
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- W4293491589 date "2022-12-01" @default.
- W4293491589 modified "2023-10-16" @default.
- W4293491589 title "Cascade-Cas3 facilitates high-accuracy genome engineering in Pseudomonas using phage-encoded homologous recombination" @default.
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- W4293491589 doi "https://doi.org/10.1016/j.engmic.2022.100046" @default.
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