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- W4293493288 abstract "Abstract Objective Alu repeats have gained huge importance in the creation and modification of regulatory networks. We previously reported a unique isoform of human CYP20A1 i.e. CYP20A1_Alu -LT with 23 Alu repeats exonized in its 9 kb long 3’UTR with 3,000 potential binding sites for 140 miRNAs. The role of this transcript was hypothesized as a potential miRNA sponge in primary neurons as its expression correlated with that of 380 genes having shared miRNA sites and enriched in neuro-coagulopathy. This study provides experimental evidence for the miRNA sponge activity of CYP20A1_Alu -LT in neuronal cell lines. Results We studied the Alu -rich fragment of the CYP20A1_Alu -LT extended 3’UTR with > 10 binding sites for miR-619-5p and miR-3677-3p. Enrichment of the Alu- rich fragment with Ago2 confirmed miRNA association of this transcript. Cloning the fragment downstream of a reporter gene led to a 90% decrease in luciferase activity. Overexpression and siRNA knockdown of CYP20A1_Alu -LT was positively correlated with the expression of miR-619-5p and miR-3677-3p target genes. GAP43 , one of the key modulators of nerve regeneration, was significantly altered by the expression of CYP20A1_Alu -LT. This study, for the first time, provides evidence for a unique regulatory function of exonized Alu repeats as miRNA sponges." @default.
- W4293493288 created "2022-08-29" @default.
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- W4293493288 date "2022-08-29" @default.
- W4293493288 modified "2023-10-17" @default.
- W4293493288 title "Exonized Alu repeats in the 3’UTR of a CYP20A1_Alu-LT transcript act as a miRNA sponge" @default.
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- W4293493288 doi "https://doi.org/10.21203/rs.3.rs-1997396/v1" @default.
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