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- W4293659823 abstract "It is well established that plant thylakoid membranes (TMs), in addition to a bilayer, contain two isotropic lipid phases and an inverted hexagonal (HII) phase. To elucidate the origin of non-bilayer lipid phases, we recorded the 31P-NMR spectra of isolated spinach plastoglobuli and TMs and tested their susceptibilities to lipases and proteases; the structural and functional characteristics of TMs were monitored using biophysical techniques and CN-PAGE. Phospholipase-A1 gradually destroyed all 31P-NMR-detectable lipid phases of isolated TMs, but the weak signal of isolated plastoglobuli was not affected. Parallel with the destabilization of their lamellar phase, TMs lost their impermeability; other effects, mainly on Photosystem-II, lagged behind the destruction of the original phases. Wheat-germ lipase selectively eliminated the isotropic phases but exerted little or no effect on the structural and functional parameters of TMs-indicating that the isotropic phases are located outside the protein-rich regions and might be involved in membrane fusion. Trypsin and Proteinase K selectively suppressed the HII phase-suggesting that a large fraction of TM lipids encapsulate stroma-side proteins or polypeptides. We conclude that-in line with the Dynamic Exchange Model-the non-bilayer lipid phases of TMs are found in subdomains separated from but interconnected with the bilayer accommodating the main components of the photosynthetic machinery." @default.
- W4293659823 created "2022-08-31" @default.
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- W4293659823 date "2022-08-28" @default.
- W4293659823 modified "2023-10-18" @default.
- W4293659823 title "Structural Entities Associated with Different Lipid Phases of Plant Thylakoid Membranes—Selective Susceptibilities to Different Lipases and Proteases" @default.
- W4293659823 cites W1547252610 @default.
- W4293659823 cites W1963807673 @default.
- W4293659823 cites W1972807122 @default.
- W4293659823 cites W1976061764 @default.
- W4293659823 cites W1978317725 @default.
- W4293659823 cites W1987843188 @default.
- W4293659823 cites W1991655565 @default.
- W4293659823 cites W1994077226 @default.
- W4293659823 cites W1994132490 @default.
- W4293659823 cites W1995766554 @default.
- W4293659823 cites W1997730487 @default.
- W4293659823 cites W2003626256 @default.
- W4293659823 cites W2003784665 @default.
- W4293659823 cites W2004163609 @default.
- W4293659823 cites W2006563307 @default.
- W4293659823 cites W2015717581 @default.
- W4293659823 cites W2023831001 @default.
- W4293659823 cites W2024860341 @default.
- W4293659823 cites W2028300852 @default.
- W4293659823 cites W2028928493 @default.
- W4293659823 cites W2029874450 @default.
- W4293659823 cites W2030855526 @default.
- W4293659823 cites W2035095410 @default.
- W4293659823 cites W2035891142 @default.
- W4293659823 cites W2040255016 @default.
- W4293659823 cites W2042132899 @default.
- W4293659823 cites W2046495643 @default.
- W4293659823 cites W2047760438 @default.
- W4293659823 cites W2060995481 @default.
- W4293659823 cites W2061794055 @default.
- W4293659823 cites W2065207721 @default.
- W4293659823 cites W2065368196 @default.
- W4293659823 cites W2073317899 @default.
- W4293659823 cites W2077996001 @default.
- W4293659823 cites W2080334952 @default.
- W4293659823 cites W2083885997 @default.
- W4293659823 cites W2084227558 @default.
- W4293659823 cites W2093869594 @default.
- W4293659823 cites W2095244250 @default.
- W4293659823 cites W2099272965 @default.
- W4293659823 cites W2099563132 @default.
- W4293659823 cites W2103730192 @default.
- W4293659823 cites W2112152621 @default.
- W4293659823 cites W2121596095 @default.
- W4293659823 cites W2122173043 @default.
- W4293659823 cites W2132741431 @default.
- W4293659823 cites W2137676881 @default.
- W4293659823 cites W2149453359 @default.
- W4293659823 cites W2150852355 @default.
- W4293659823 cites W2153353543 @default.
- W4293659823 cites W2154663309 @default.
- W4293659823 cites W2164188684 @default.
- W4293659823 cites W2171093931 @default.
- W4293659823 cites W2204291048 @default.
- W4293659823 cites W2232724257 @default.
- W4293659823 cites W2247049448 @default.
- W4293659823 cites W2319314380 @default.
- W4293659823 cites W2319658814 @default.
- W4293659823 cites W2334503806 @default.
- W4293659823 cites W2342957814 @default.
- W4293659823 cites W2345912299 @default.
- W4293659823 cites W2398071544 @default.
- W4293659823 cites W2484137621 @default.
- W4293659823 cites W2549914328 @default.
- W4293659823 cites W2733857666 @default.
- W4293659823 cites W2764210815 @default.
- W4293659823 cites W2769523034 @default.
- W4293659823 cites W2769532020 @default.
- W4293659823 cites W2771471573 @default.
- W4293659823 cites W2784151735 @default.
- W4293659823 cites W2784976431 @default.
- W4293659823 cites W2788577231 @default.
- W4293659823 cites W2807483966 @default.
- W4293659823 cites W2912709084 @default.
- W4293659823 cites W2913784474 @default.
- W4293659823 cites W2980799986 @default.
- W4293659823 cites W2981920787 @default.
- W4293659823 cites W2998921455 @default.