Matches in SemOpenAlex for { <https://semopenalex.org/work/W4293723997> ?p ?o ?g. }
- W4293723997 endingPage "9829" @default.
- W4293723997 startingPage "9829" @default.
- W4293723997 abstract "The sex and APOE4 genotype are significant risk factors for Alzheimer’s disease (AD); however, the mechanism(s) responsible for this interaction are still a matter of debate. Here, we assess the responses of mixed-sex and sex-specific APOE3 and APOE4 primary microglia (PMG) to lipopolysaccharide and interferon-gamma. In our investigation, inflammatory cytokine profiles were assessed by qPCR and multiplex ELISA assays. Mixed-sex APOE4 PMG exhibited higher basal mRNA expression and secreted levels of TNFa and IL1b. In sex-specific cultures, basal expression and secreted levels of IL1b, TNFa, IL6, and NOS2 were 2−3 fold higher in APOE4 female PMG compared to APOE4 males, with both higher than APOE3 cells. Following an inflammatory stimulus, the expression of pro-inflammatory cytokines and the secreted cytokine level were upregulated in the order E4 female > E4 male > E3 female > E3 male in sex-specific cultures. These data indicate that the APOE4 genotype and female sex together contribute to a greater inflammatory response in PMG isolated from targeted replacement humanized APOE mice. These data are consistent with clinical data and indicate that sex-specific PMG may provide a platform for exploring mechanisms of genotype and sex differences in AD related to neuroinflammation and neurodegeneration." @default.
- W4293723997 created "2022-08-31" @default.
- W4293723997 creator A5000316446 @default.
- W4293723997 creator A5002790795 @default.
- W4293723997 creator A5016661815 @default.
- W4293723997 creator A5026746873 @default.
- W4293723997 creator A5056724620 @default.
- W4293723997 date "2022-08-29" @default.
- W4293723997 modified "2023-10-14" @default.
- W4293723997 title "Sex and APOE Genotype Alter the Basal and Induced Inflammatory States of Primary Microglia from APOE Targeted Replacement Mice" @default.
- W4293723997 cites W1500145074 @default.
- W4293723997 cites W1584106444 @default.
- W4293723997 cites W1862417723 @default.
- W4293723997 cites W1884934928 @default.
- W4293723997 cites W1916233725 @default.
- W4293723997 cites W1973065853 @default.
- W4293723997 cites W1977434686 @default.
- W4293723997 cites W1984737558 @default.
- W4293723997 cites W1999631936 @default.
- W4293723997 cites W1999794509 @default.
- W4293723997 cites W2001618696 @default.
- W4293723997 cites W2011885704 @default.
- W4293723997 cites W2013863433 @default.
- W4293723997 cites W2018109459 @default.
- W4293723997 cites W2019786269 @default.
- W4293723997 cites W2021181758 @default.
- W4293723997 cites W2033105784 @default.
- W4293723997 cites W2033883349 @default.
- W4293723997 cites W2034691653 @default.
- W4293723997 cites W2034968148 @default.
- W4293723997 cites W2036362108 @default.
- W4293723997 cites W2045317765 @default.
- W4293723997 cites W2045663319 @default.
- W4293723997 cites W2049733019 @default.
- W4293723997 cites W2051212645 @default.
- W4293723997 cites W2055766521 @default.
- W4293723997 cites W2056516296 @default.
- W4293723997 cites W2058034919 @default.
- W4293723997 cites W2058120050 @default.
- W4293723997 cites W2064179601 @default.
- W4293723997 cites W2069121057 @default.
- W4293723997 cites W2070419177 @default.
- W4293723997 cites W2080378222 @default.
- W4293723997 cites W2090271811 @default.
- W4293723997 cites W2094912260 @default.
- W4293723997 cites W2096142564 @default.
- W4293723997 cites W2103030628 @default.
- W4293723997 cites W2110616131 @default.
- W4293723997 cites W2114570899 @default.
- W4293723997 cites W2115738813 @default.
- W4293723997 cites W2115779804 @default.
- W4293723997 cites W2121536490 @default.
- W4293723997 cites W2121733151 @default.
- W4293723997 cites W2135140141 @default.
- W4293723997 cites W2140718540 @default.
- W4293723997 cites W2143955562 @default.
- W4293723997 cites W2144373514 @default.
- W4293723997 cites W2147413218 @default.
- W4293723997 cites W2159285109 @default.
- W4293723997 cites W2162941124 @default.
- W4293723997 cites W2166511841 @default.
- W4293723997 cites W2191310828 @default.
- W4293723997 cites W2261364877 @default.
- W4293723997 cites W2261699367 @default.
- W4293723997 cites W2270987730 @default.
- W4293723997 cites W2316785311 @default.
- W4293723997 cites W2327790265 @default.
- W4293723997 cites W2521392402 @default.
- W4293723997 cites W2528438579 @default.
- W4293723997 cites W2554957493 @default.
- W4293723997 cites W256414136 @default.
- W4293723997 cites W2567387238 @default.
- W4293723997 cites W2568317134 @default.
- W4293723997 cites W2591809865 @default.
- W4293723997 cites W2600450442 @default.
- W4293723997 cites W2609539430 @default.
- W4293723997 cites W2617930056 @default.
- W4293723997 cites W2619332462 @default.
- W4293723997 cites W2622807556 @default.
- W4293723997 cites W2652911668 @default.
- W4293723997 cites W2731315321 @default.
- W4293723997 cites W2735527928 @default.
- W4293723997 cites W2737955012 @default.
- W4293723997 cites W2754890392 @default.
- W4293723997 cites W2766502684 @default.
- W4293723997 cites W2770304782 @default.
- W4293723997 cites W2773854938 @default.
- W4293723997 cites W2789258764 @default.
- W4293723997 cites W2795435173 @default.
- W4293723997 cites W2797930701 @default.
- W4293723997 cites W2801203507 @default.
- W4293723997 cites W2803902280 @default.
- W4293723997 cites W2804685648 @default.
- W4293723997 cites W2805798400 @default.
- W4293723997 cites W2808882111 @default.
- W4293723997 cites W2885498250 @default.
- W4293723997 cites W2891379852 @default.
- W4293723997 cites W2893415830 @default.