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- W4293777667 abstract "Japanese flounder (Paralichthys olivaceus) responsive mechanisms to hypoxia are still not fully understood. Therefore, we performed an acute hypoxic treatment (dissolved oxygen at 2.07 ± 0.08 mg/L) on Japanese flounder. It was confirmed that the hypoxic stress affected the physiological phenotype through changes in blood physiology (RBC, HGB, WBC), biochemistry (LDH, ALP, ALT, GLU, TC, TG, ALB), and hormone (cortisol) indicators. Hypoxia inducible factor-1 (HIF-1), an essential oxygen homeostasis mediator in organisms consisting of an inducible HIF-1α and a constitutive HIF-1β, and its target gene LDH-A were deeply studied. Results showed that HIF-1α and LDH-A genes were co-expressed and significantly affected by hypoxic stress. The dual-luciferase reporter assay confirmed that transcription factor HIF-1 transcriptionally regulated the LDH-A gene, and its transcription binding sequence was GGACGTGA located at -2343~-2336. The DNA methylation status of HIF-1α and LDH-A genes were detected to understand the mechanism of environmental stress on genes. It was found that hypoxia affected the HIF-1α gene and LDH-A gene methylation levels. The study uncovered HIF-1/LDH-A signaling pathway responsive mechanisms of Japanese flounder to hypoxia in epigenetic modification and transcriptional regulation. Our study is significant to further the understanding of environmental responsive mechanisms as well as providing a reference for aquaculture." @default.
- W4293777667 created "2022-08-31" @default.
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- W4293777667 date "2022-08-18" @default.
- W4293777667 modified "2023-10-14" @default.
- W4293777667 title "Hypoxia Affects HIF-1/LDH-A Signaling Pathway by Methylation Modification and Transcriptional Regulation in Japanese Flounder (Paralichthys olivaceus)" @default.
- W4293777667 cites W1503667331 @default.
- W4293777667 cites W1509040210 @default.
- W4293777667 cites W1529455781 @default.
- W4293777667 cites W1657609940 @default.
- W4293777667 cites W180388768 @default.
- W4293777667 cites W1807437640 @default.
- W4293777667 cites W1970164206 @default.
- W4293777667 cites W1977772936 @default.
- W4293777667 cites W1978983799 @default.
- W4293777667 cites W1979551064 @default.
- W4293777667 cites W1982690749 @default.
- W4293777667 cites W1983798255 @default.
- W4293777667 cites W1987910531 @default.
- W4293777667 cites W1992548312 @default.
- W4293777667 cites W1995363474 @default.
- W4293777667 cites W1997992120 @default.
- W4293777667 cites W2000086275 @default.
- W4293777667 cites W2001395726 @default.
- W4293777667 cites W2010394923 @default.
- W4293777667 cites W2016934775 @default.
- W4293777667 cites W2020002300 @default.
- W4293777667 cites W2021041806 @default.
- W4293777667 cites W2026284310 @default.
- W4293777667 cites W2038522437 @default.
- W4293777667 cites W2042216712 @default.
- W4293777667 cites W2042443353 @default.
- W4293777667 cites W2044785068 @default.
- W4293777667 cites W2049114614 @default.
- W4293777667 cites W2054137210 @default.
- W4293777667 cites W2054523001 @default.
- W4293777667 cites W2056897758 @default.
- W4293777667 cites W2057206284 @default.
- W4293777667 cites W2058124913 @default.
- W4293777667 cites W2059886280 @default.
- W4293777667 cites W2066636897 @default.
- W4293777667 cites W2070109031 @default.
- W4293777667 cites W2071967139 @default.
- W4293777667 cites W2082540577 @default.
- W4293777667 cites W2084410329 @default.
- W4293777667 cites W2084953314 @default.
- W4293777667 cites W2089815674 @default.
- W4293777667 cites W2090786698 @default.
- W4293777667 cites W2091413411 @default.
- W4293777667 cites W2094129195 @default.
- W4293777667 cites W2099824042 @default.
- W4293777667 cites W2110251906 @default.
- W4293777667 cites W2114496296 @default.
- W4293777667 cites W2121417064 @default.
- W4293777667 cites W2124032276 @default.
- W4293777667 cites W2126763338 @default.
- W4293777667 cites W2139576132 @default.
- W4293777667 cites W2143343444 @default.
- W4293777667 cites W2145919968 @default.
- W4293777667 cites W2150350398 @default.
- W4293777667 cites W2153160772 @default.
- W4293777667 cites W2154995504 @default.
- W4293777667 cites W2158794894 @default.
- W4293777667 cites W2160325871 @default.
- W4293777667 cites W2168615744 @default.
- W4293777667 cites W2171502870 @default.
- W4293777667 cites W2183044194 @default.
- W4293777667 cites W2226989783 @default.
- W4293777667 cites W2296918553 @default.
- W4293777667 cites W2311435523 @default.
- W4293777667 cites W2316727342 @default.
- W4293777667 cites W2513656963 @default.
- W4293777667 cites W2607132421 @default.
- W4293777667 cites W2610903912 @default.
- W4293777667 cites W2613855915 @default.
- W4293777667 cites W2620406790 @default.
- W4293777667 cites W2622156299 @default.
- W4293777667 cites W2623459795 @default.
- W4293777667 cites W2626493603 @default.
- W4293777667 cites W2738551041 @default.
- W4293777667 cites W2748742677 @default.
- W4293777667 cites W2764045072 @default.
- W4293777667 cites W2770854880 @default.
- W4293777667 cites W2783193920 @default.
- W4293777667 cites W2792987386 @default.
- W4293777667 cites W2805529846 @default.
- W4293777667 cites W2806227083 @default.
- W4293777667 cites W2807639370 @default.
- W4293777667 cites W2895206108 @default.
- W4293777667 cites W2908508599 @default.