FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4294002012> ?p ?o ?g. }

• W4294002012 endingPage "155" @default.
• W4294002012 startingPage "147" @default.
• W4294002012 abstract "Protein aggregates are often varying extensively in their morphological characteristics, which may lead to various biological outcomes, such as increased immunogenicity risk. However, isolation of aggregates with a specific morphology within an ensemble is often challenging. To gain vital knowledge on the effects of aggregate characteristics, samples containing a single morphology must be produced by direct control of the aggregation process. Moreover, the formed aggregates need to be in an aqueous solution suitable for biological assays, while keeping their morphology intact. Here we evaluated the dependence of morphology and integrity of amyloid-like fibrils and spherulites on preparation conditions and post-treatment methods. Samples containing either amyloid-like fibrils or spherulites produced from human insulin in acetic acid solutions are dependent on the presence of salt (NaCl). Moreover, mechanical shaking (600 rpm) inhibits spherulite formation, while only affecting the length of the formed fibrils compared to quiescent conditions. Besides shaking, the initial protein concentration in the formulation was found to control fibril length. Surprisingly, exchanging the solution used for aggregate formation to a physiologically relevant buffer, had a striking effect on the morphological integrity of the fibril and spherulite samples. Especially the secondary structure of one of our spherulite samples presented dramatic changes of the aggregated β-sheet content after exchanging the solution, emphasizing the importance of the aggregate stability. These results and considerations have profound implications on the data interpretation and should be implemented in the workflow for both fundamental characterization of aggregates as well as assays for evaluation of their corresponding biological effects." @default.
• W4294002012 created "2022-09-01" @default.
• W4294002012 creator A5010054439 @default.
• W4294002012 creator A5022872308 @default.
• W4294002012 creator A5040804836 @default.
• W4294002012 creator A5046807792 @default.
• W4294002012 creator A5079620567 @default.
• W4294002012 creator A5084562827 @default.
• W4294002012 date "2022-10-01" @default.
• W4294002012 modified "2023-10-18" @default.
• W4294002012 title "Morphological integrity of insulin amyloid-like aggregates depends on preparation methods and post-production treatments" @default.
• W4294002012 cites W1816340581 @default.
• W4294002012 cites W1974790276 @default.
• W4294002012 cites W1980333958 @default.
• W4294002012 cites W1984034271 @default.
• W4294002012 cites W1999853753 @default.
• W4294002012 cites W1999856601 @default.
• W4294002012 cites W2004774375 @default.
• W4294002012 cites W2006503638 @default.
• W4294002012 cites W2008289288 @default.
• W4294002012 cites W2010462701 @default.
• W4294002012 cites W2027780480 @default.
• W4294002012 cites W2029174947 @default.
• W4294002012 cites W2038913139 @default.
• W4294002012 cites W2048930756 @default.
• W4294002012 cites W2057524757 @default.
• W4294002012 cites W2071095038 @default.
• W4294002012 cites W2072118579 @default.
• W4294002012 cites W2078172351 @default.
• W4294002012 cites W2085899859 @default.
• W4294002012 cites W2088395760 @default.
• W4294002012 cites W2138698547 @default.
• W4294002012 cites W2148892150 @default.
• W4294002012 cites W2150411892 @default.
• W4294002012 cites W2281597363 @default.
• W4294002012 cites W2309356304 @default.
• W4294002012 cites W2316189327 @default.
• W4294002012 cites W2319662570 @default.
• W4294002012 cites W2322540009 @default.
• W4294002012 cites W2615682841 @default.
• W4294002012 cites W2769603921 @default.
• W4294002012 cites W2790341450 @default.
• W4294002012 cites W2791176268 @default.
• W4294002012 cites W2806876262 @default.
• W4294002012 cites W2898192685 @default.
• W4294002012 cites W2981524936 @default.
• W4294002012 cites W2997957740 @default.
• W4294002012 cites W3014541697 @default.
• W4294002012 cites W3042959081 @default.
• W4294002012 cites W3128086321 @default.
• W4294002012 cites W3203793415 @default.
• W4294002012 cites W4200428589 @default.
• W4294002012 cites W4223913930 @default.
• W4294002012 cites W4292430051 @default.
• W4294002012 cites W4307642786 @default.
• W4294002012 doi "https://doi.org/10.1016/j.ejpb.2022.08.018" @default.
• W4294002012 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36058445" @default.
• W4294002012 hasPublicationYear "2022" @default.
• W4294002012 type Work @default.
• W4294002012 citedByCount "2" @default.
• W4294002012 countsByYear W42940020122023 @default.
• W4294002012 crossrefType "journal-article" @default.
• W4294002012 hasAuthorship W4294002012A5010054439 @default.
• W4294002012 hasAuthorship W4294002012A5022872308 @default.
• W4294002012 hasAuthorship W4294002012A5040804836 @default.
• W4294002012 hasAuthorship W4294002012A5046807792 @default.
• W4294002012 hasAuthorship W4294002012A5079620567 @default.
• W4294002012 hasAuthorship W4294002012A5084562827 @default.
• W4294002012 hasBestOaLocation W42940020121 @default.
• W4294002012 hasConcept C12554922 @default.
• W4294002012 hasConcept C126322002 @default.
• W4294002012 hasConcept C127413603 @default.
• W4294002012 hasConcept C136238340 @default.
• W4294002012 hasConcept C171250308 @default.
• W4294002012 hasConcept C178790620 @default.
• W4294002012 hasConcept C179104552 @default.
• W4294002012 hasConcept C185592680 @default.
• W4294002012 hasConcept C192562407 @default.
• W4294002012 hasConcept C203036418 @default.
• W4294002012 hasConcept C27523624 @default.
• W4294002012 hasConcept C2777633098 @default.
• W4294002012 hasConcept C2779134260 @default.
• W4294002012 hasConcept C2780841128 @default.
• W4294002012 hasConcept C2994168385 @default.
• W4294002012 hasConcept C3019447875 @default.
• W4294002012 hasConcept C42360764 @default.
• W4294002012 hasConcept C499950583 @default.
• W4294002012 hasConcept C54355233 @default.
• W4294002012 hasConcept C55493867 @default.
• W4294002012 hasConcept C5556706 @default.
• W4294002012 hasConcept C71924100 @default.
• W4294002012 hasConcept C86803240 @default.
• W4294002012 hasConceptScore W4294002012C12554922 @default.
• W4294002012 hasConceptScore W4294002012C126322002 @default.
• W4294002012 hasConceptScore W4294002012C127413603 @default.
• W4294002012 hasConceptScore W4294002012C136238340 @default.
• W4294002012 hasConceptScore W4294002012C171250308 @default.
• W4294002012 hasConceptScore W4294002012C178790620 @default.

• next